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To determine whether pp60<jats:sup>c\u2010src<\/jats:sup> is reexpressed in regenerating axons, its expression was studied by immunoblotting and immunocytochemical analyses in adult chicken sciatic nerve following nerve crush injury. pp60<jats:sup>c\u2010src<\/jats:sup> expression was found to increase during nerve repair with a temporal and spatial pattern consistent with a localization in regenerating axons. At the crush site, pp60<jats:sup>c\u2010src<\/jats:sup> increased to maximal levels 7 days postinjury, increasing fivefold relative to 0 day nerve. In the nerve segment distal to the injury, the maximal increase in pp60<jats:sup>c\u2010src<\/jats:sup> was sevenfold and occurred between 11 and 21 days postinjury. Immunoperoxidase staining revealed pp60<jats:sup>c\u2010src<\/jats:sup> in regenerating axons and certain nonneuronal cells at the site of nerve repair. pp60<jats:sup>c\u2010src<\/jats:sup> was induced in both motor and sensory neurons, as shown by increased pp60<jats:sup>c\u2010src<\/jats:sup> immunoreactivity in their cell bodies located in the spinal cord and dorsal root ganglion. Phosphotyrosine\u2010modified proteins that were potential targets of pp60<jats:sup>c\u2010src<\/jats:sup> increased following nerve crush, and were localized to outgrowing neurites as well as to nonneuronal cells. These results suggest that pp60<jats:sup>c\u2010src<\/jats:sup> is a common component of cellular mechanisms regulating growth cone migration in both regenerating and developing axons.<\/jats:p>","DOI":"10.1002\/cne.903150205","type":"journal-article","created":{"date-parts":[[2005,1,1]],"date-time":"2005-01-01T22:52:57Z","timestamp":1104619977000},"page":"171-177","source":"Crossref","is-referenced-by-count":16,"title":["Altered expression of pp60<sup>c\u2010src<\/sup> induced by peripheral nerve injury"],"prefix":"10.1002","volume":"315","author":[{"suffix":"Jr.","given":"Michael A.","family":"Ignelzi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephanie S.","family":"Padilla","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daryl E.","family":"Warder","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patricia F.","family":"Maness","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,10,9]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/298084a0"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.3.3.304"},{"key":"e_1_2_1_4_1","first-page":"2019","volume-title":"The Anatomy of Domestic Animals","author":"Baumel J. 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