{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T15:40:05Z","timestamp":1762270805318},"reference-count":40,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2004,10,9]],"date-time":"2004-10-09T00:00:00Z","timestamp":1097280000000},"content-version":"vor","delay-in-days":4370,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Comparative Neurology"],"published-print":{"date-parts":[[1992,10,22]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The primary structure of frog pituitary adenylate cyclase\u2010activating polypeptide (PACAP) has recently been determined and the results show that the sequence of PACAP has been highly conserved during evolution. In particular, the structure of the 1\u201327 fragment of PACAP is identical in frog and mammals. Using an antiserum raised against PACAP27, we have investigated the distribution of PACAP\u2010containing neurons in the central nervous system of the frog <jats:italic>Rana ridibunda<\/jats:italic> by the immunofluorescence technique. The main populations of immunoreactive perikarya were located in the medial and ventral diencephalon, i.e., the preoptic nucleus, the ventral and dorsal infundibular nuclei, the nucleus posterocentralis thalami, and the ventral and ventrolateral areas of the thalamus. In the telencephalon, sparse cell bodies were found in the nucleus accumbens septi, the amygdala, the pallial commissure, and the bed nucleus of the pallial commissure. In the hindbrain, the torus semicircularis, the nucleus profundus and the nucleus anteroventralis tegmenti of the mesencephalon also contained populations of PACAP\u2010immunoreactive perikarya. Beaded nerve fibers were observed throughout the brain. Occasionally they formed bundles, e.g., from the ventral infundibulum to the external vascular layer of the median eminence, from the central thalamus to the optic tectum, and rostrocaudally, from the nucleus accumbens septi to the nucleus entopeduncularis. Other areas, such as the interpeduncular nucleus, the nucleus isthmi and the roots of cranial nerves V and VIII in the medulla oblongata, were also densely innervated. The adenylate cyclase\u2010stimulating activity of PACAP was tested by using a static incubation technique for hypothalamic slices. Synthetic PACAP27 (lO<jats:sup>\u221210<\/jats:sup> to 3 \u00b7 10<jats:sup>\u22126<\/jats:sup> M) induced a dose\u2010dependent stimulation of cAMP production. The half\u2010maximum effective dose (ED<jats:sub>50<\/jats:sub>) of PACAP27was3.5 \u00b7 10<jats:sup>\u22127<\/jats:sup> M.<\/jats:p><jats:p>Taken together, these results provide the first evidence for the presence of PACAP in the central nervous system of nonmammalian vertebrates. The discrete localization of PACAP immunoreactivity in the brain and the adenylate cyclase\u2010stimulating activity of PACAP27 on frog hypothalamic slices suggest that PACAP acts as a neurotransmitter or neuromodulator in the brain of amphibians.<\/jats:p>","DOI":"10.1002\/cne.903240403","type":"journal-article","created":{"date-parts":[[2005,5,27]],"date-time":"2005-05-27T21:49:05Z","timestamp":1117230545000},"page":"485-499","source":"Crossref","is-referenced-by-count":65,"title":["Immunohistochemical distribution and biological activity of pituitary adenylate cyclase\u2010activating polypeptide (PACAP) in the central nervous system of the frog <i>Rana ridibunda<\/i>"],"prefix":"10.1002","volume":"324","author":[{"given":"Laurent","family":"Yon","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marc","family":"Feuilloley","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicolas","family":"Chartrel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akira","family":"Arimura","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. Michael","family":"Conlon","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alain","family":"Fournier","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hubert","family":"Vaudry","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,10,9]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0361-9230(87)90198-5"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(86)91300-4"},{"key":"e_1_2_1_4_1","first-page":"287","volume-title":"Basic Neurochemistry","author":"Brownstein M.J.","year":"1989"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(90)80158-F"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.130.1.211"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0196-9781(90)90194-A"},{"key":"e_1_2_1_8_1","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1016\/0167-0115(91)90478-Y","article-title":"Properties and distribution of receptors for pituitary adenylate cyclase\u2010activating peptide (PACAP) in rat brain and spinal cord. 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