{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,30]],"date-time":"2025-10-30T11:12:33Z","timestamp":1761822753596},"reference-count":61,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2004,10,9]],"date-time":"2004-10-09T00:00:00Z","timestamp":1097280000000},"content-version":"vor","delay-in-days":4254,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Comparative Neurology"],"published-print":{"date-parts":[[1993,2,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Somatic motor neurons begin to express the transmitter synthesizing enzyme, choline acetyltransferase (ChAT) and the low\u2010affinity nerve growth factor receptor (NGFR) during embryonic development. However, as motor neurons mature in postnatal life, they lose immunoreactivity for NGFR and acquire a motor neuron\u2010specific epitope that is recognized by the monoclonal antibody, MO\u20101. The present study was undertaken to examine the effect of nerve injury in adult rats on these three developmentally regulated markers in two populations of somatic motor neurons.<\/jats:p><jats:p>Unilateral transection, ligation, or crushing of the sciatic nerve resulted in a loss of MO\u20101 binding and a concomitant rise in immunoreactivity for NGFR within axotomized motor neurons in lumbar levels of the spinal cord. These changes, detectable within 5 days following nerve injury, are reversed with reinnervation, but persist if reinnervation is prevented by chronic axotomy. Thus, regulation of the expression of NGFR and the MO\u20101 epitope appears to be critically dependent upon interactions between motor neurons and target muscles. These observations are also consistent with the idea that during regeneration, neurons may revert to a developmentally immature state; in motor neurons, this state is characterized by the presence of NGFRs and the absence of the MO\u20101 epitope.<\/jats:p><jats:p>Transection of the hypoglossal nerve, a purely motor nerve, resulted in a similar loss of MO\u20101 binding and a selective rise in NGFR immunoreactivity in neurons within the ipsilateral hypoglossal motor nucleus. In addition immunoreactivity for ChAT was also lost in axotomized hypoglossal motor neurons. In contrast, injury to the sciatic nerve, which bears both sensory and motor axons, did not result in any detectable change in ChAT immunoreactivity in spinal motor neurons. \u00a9 1993 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/cne.903280303","type":"journal-article","created":{"date-parts":[[2005,1,1]],"date-time":"2005-01-01T08:40:35Z","timestamp":1104568835000},"page":"351-363","source":"Crossref","is-referenced-by-count":31,"title":["A motor neuron\u2010specific epitope and the low\u2010affinity nerve growth factor receptor display reciprocal patterns of during development, axotomy, and regeneration"],"prefix":"10.1002","volume":"328","author":[{"given":"Arlene Y.","family":"Chiu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edmond W.","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sofia","family":"Loera","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,10,9]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.10-11-03507.1990"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.903040407"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(91)90540-F"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)39810-1"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.903200303"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.79.22.7031"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.10-05-01643.1990"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(77)90702-8"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(84)90937-5"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(92)90180-K"},{"key":"e_1_2_1_12_1","doi-asserted-by":"crossref","first-page":"412","DOI":"10.1523\/JNEUROSCI.11-02-00412.1991","article-title":"Acidic and basic fibroblast growth factors in the nervous system: Distribution and differential alteration of levels after injury of central versus peripheral nerve","volume":"11","author":"Eckenstein F. P.","year":"1991","journal-title":"J. Neurosc."},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/0896-6273(89)90049-4"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1991.tb07833.x"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/0166-2236(92)90022-Z"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/350678a0"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1242\/jeb.132.1.133"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(91)90614-5"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1850549"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(91)90471-7"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-510X(86)90109-7"},{"key":"e_1_2_1_22_1","doi-asserted-by":"crossref","first-page":"969","DOI":"10.1016\/0092-8674(91)90442-2","article-title":"trkC, a new member of the trk family of tyrosine protein kinases, is a receptor for neurotrophin\u20103","volume":"66","author":"Lamballe F.","year":"1991","journal-title":"Cell"},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(88)90635-X"},{"key":"e_1_2_1_24_1","doi-asserted-by":"crossref","first-page":"2451","DOI":"10.1523\/JNEUROSCI.10-07-02451.1990","article-title":"Lineage, arrangement and death of clonally related neurons in chick spinal cord","volume":"10","author":"Leber S. M.","year":"1990","journal-title":"J. Neurosci."},{"key":"e_1_2_1_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/0166-2236(92)90014-Y"},{"key":"e_1_2_1_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/0167-5699(91)90033-P"},{"key":"e_1_2_1_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1990.tb08211.x"},{"key":"e_1_2_1_28_1","first-page":"45","article-title":"Cholinergic differentiation factor (CDG\/LIF) promotes choline acetyltransferase activity in rat spinal motoneurons","volume":"4","author":"Martinou J.\u2010C.","year":"1991","journal-title":"Third IBRO World Congress of Neuroscience"},{"key":"e_1_2_1_29_1","doi-asserted-by":"crossref","first-page":"3645","DOI":"10.1523\/JNEUROSCI.09-10-03645.1989","article-title":"Characterization of two factors enhancing choline acetyltransferase activity in cultures of purified rat motoneurons","volume":"9","author":"Martinou J.\u2010C.","year":"1989","journal-title":"J. Neurosci."},{"key":"e_1_2_1_30_1","doi-asserted-by":"crossref","first-page":"5890","DOI":"10.1016\/S0021-9258(18)60649-0","article-title":"Purification of a skeletal muscle polypeptide which stimulates choline acetyltransferase activity in cultured spinal cord neurons","volume":"263","author":"McManaman J. L.","year":"1988","journal-title":"J. Biol. Chem."},{"key":"e_1_2_1_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.1989.tb09241.x"},{"key":"e_1_2_1_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/0896-6273(90)90142-3"},{"key":"e_1_2_1_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(91)91371-7"},{"key":"e_1_2_1_34_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.902170107"},{"key":"e_1_2_1_35_1","first-page":"227","article-title":"Fibre composition of the hypoglossal nerve in the rat","volume":"172","author":"O'Reilly P. M. R.","year":"1990","journal-title":"J. Anat."},{"key":"e_1_2_1_36_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.902730403"},{"key":"e_1_2_1_37_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1002\/cne.903070108","article-title":"Embryonic development of choline acetyltransferase in thoracic spinal motor neurons: Somatic and autonomic neurons may be derived from a common cellular group","volume":"307","author":"Phelps P. E.","year":"1991","journal-title":"J. Comp. Neurol."},{"key":"e_1_2_1_38_1","doi-asserted-by":"crossref","first-page":"286","DOI":"10.1002\/cne.902380305","article-title":"Immunocytochemical localization of choline acetyltransferase within the rat neostriatum: A light and electron microscopic study of cholinergic neurons and synapses","volume":"283","author":"Phelps P. E.","year":"1985","journal-title":"J. Comp. Neurol."},{"key":"e_1_2_1_39_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.902910103"},{"key":"e_1_2_1_40_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.112.1.83"},{"key":"e_1_2_1_41_1","doi-asserted-by":"publisher","DOI":"10.1002\/glia.440050105"},{"key":"e_1_2_1_42_1","doi-asserted-by":"publisher","DOI":"10.1098\/rstb.1991.0013"},{"key":"e_1_2_1_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/0169-328X(91)90142-K"},{"key":"e_1_2_1_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(90)90816-W"},{"key":"e_1_2_1_45_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.902240206"},{"key":"e_1_2_1_46_1","doi-asserted-by":"publisher","DOI":"10.1038\/345440a0"},{"key":"e_1_2_1_47_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.118.1.139"},{"key":"e_1_2_1_48_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.2160731"},{"key":"e_1_2_1_49_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.902790312"},{"key":"e_1_2_1_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(91)90396-G"},{"key":"e_1_2_1_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/0896-6273(90)90334-C"},{"key":"e_1_2_1_52_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1989.tb03521.x"},{"key":"e_1_2_1_53_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.115.2.447"},{"key":"e_1_2_1_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4886(91)90087-S"},{"key":"e_1_2_1_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/0166-2236(91)90097-E"},{"key":"e_1_2_1_56_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.84.15.5459"},{"key":"e_1_2_1_57_1","doi-asserted-by":"crossref","first-page":"620","DOI":"10.1523\/JNEUROSCI.10-02-00620.1990","article-title":"A monoclonal antibody that recognizes somatic motor neurons in the mature rat nervous system","volume":"10","author":"Urakami H.","year":"1990","journal-title":"J. Neurosci."},{"key":"e_1_2_1_58_1","doi-asserted-by":"publisher","DOI":"10.1016\/0736-5748(84)90009-1"},{"key":"e_1_2_1_59_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1460-9568.1990.tb00454.x"},{"key":"e_1_2_1_60_1","doi-asserted-by":"publisher","DOI":"10.1016\/0012-1606(87)90147-3"},{"key":"e_1_2_1_61_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.08-09-03481.1988"},{"key":"e_1_2_1_62_1","doi-asserted-by":"publisher","DOI":"10.1016\/0896-6273(88)90082-7"}],"container-title":["Journal of Comparative Neurology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcne.903280303","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cne.903280303","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,25]],"date-time":"2023-10-25T19:29:44Z","timestamp":1698262184000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cne.903280303"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1993,2,15]]},"references-count":61,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1993,2,15]]}},"alternative-id":["10.1002\/cne.903280303"],"URL":"https:\/\/doi.org\/10.1002\/cne.903280303","archive":["Portico"],"relation":{},"ISSN":["0021-9967","1096-9861"],"issn-type":[{"value":"0021-9967","type":"print"},{"value":"1096-9861","type":"electronic"}],"subject":[],"published":{"date-parts":[[1993,2,15]]}}}