{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T12:54:47Z","timestamp":1761742487402},"reference-count":29,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2004,10,9]],"date-time":"2004-10-09T00:00:00Z","timestamp":1097280000000},"content-version":"vor","delay-in-days":4219,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Comparative Neurology"],"published-print":{"date-parts":[[1993,3,22]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>To elucidate the role of neuron\u2010glia cell adhesion molecule (Ng\u2010CAM) in axonal pathway formation of avian spinal interneurons, we have examined the ultrastructural expression of Ng\u2010CAM in the developing spinal cord, by using a preembedding immunocytochemical method. Ng\u2010CAM immunoreactivity was punctate and was restricted to cell surfaces. In accordance with our previous light microscopic observations (Shiga et al., '90), the earliest developing spinal interneurons were Ng\u2010CAM\u2010positive on their cell bodies, axons, and growth cones. Axons and growth cones that were either fasciculated or in contact with each other strongly expressed Ng\u2010CAM, thus indicating the possible involvement of Ng\u2010CAM in fasciculation of axons and in the contact guidance of growth cones along preexisting axons. By using higher resolution immunoelectron microscopy, the present study has also revealed new information on the subcellular localization of Ng\u2010CAM on developing spinal interneurons, neuroepithelial cells, and floor plate cells. Although Ng\u2010CAM immunoreactivity was prominent on both axons and growth cones, these structures were Ng\u2010CAM\u2010negative when they contacted the basal lamina around the spinal cord. By contrast, Ng\u2010CAM was detectable on the surface of both neuroepithelial cells and floor plate cells only when they made contact with the Ng\u2010CAM\u2010positive axons and growth cones of interneurons. These results suggest that the subcellular distribution of Ng\u2010CAM is regulated differentially, depending on the apposing cell surfaces, and that such differential and developmentally regulated expression may contribute to the elongation, fasciculation, and guidance of spinal axons. \u00a9 1993 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/cne.903290407","type":"journal-article","created":{"date-parts":[[2005,1,1]],"date-time":"2005-01-01T08:43:19Z","timestamp":1104568999000},"page":"512-518","source":"Crossref","is-referenced-by-count":9,"title":["Differential expression of neuron\u2010glia cell adhesion molecule (Ng\u2010CAM) on developing axons and growth cones of interneurons in the chick embryo spinal cord: An immunoelectron microscopic study"],"prefix":"10.1002","volume":"329","author":[{"given":"Taskashi","family":"Shiga","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Toshio","family":"Shirai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martin","family":"Grumet","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gerald M.","family":"Edelman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ronald W.","family":"Oppenheim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,10,9]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.902840310"},{"key":"e_1_2_1_3_1","doi-asserted-by":"crossref","first-page":"1027","DOI":"10.1083\/jcb.112.5.1017","article-title":"Structure of the chicken neuron\u2010glia cell adhesion molecule, Ng\u2010CAM: Origin of the polypeptides and relation to the Ig superfamily","volume":"112","author":"Burgoon M. 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