{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T19:48:46Z","timestamp":1762112926096},"reference-count":116,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2004,10,9]],"date-time":"2004-10-09T00:00:00Z","timestamp":1097280000000},"content-version":"vor","delay-in-days":4073,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Comparative Neurology"],"published-print":{"date-parts":[[1993,8,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Dorsal root ganglion (DRG) neurons decrease their substance P (SP) synthesis after peripheral nerve lesions. Levels in the dorsal horn also decline but return to normal if regeneration is successful. In adults, when regeneration is prevented, recovery of SP in the dorsal horn is slow and incomplete, whereas in newborns, recovery is rapid and complete even though retrograde cell death of DRG neurons is greater than in adults. We have examined the mechanisms that might account for the rapid and complete recovery of SP and calcitonin\u2010gene related peptide (CGRP) in the dorsal horn after peripheral nerve injury in newborns. Peptides were compared in the L4 and L5 DRG and spinal cord segments of normal rats and in rats surviving 6 days to 4 months after sciatic nerve section\/ligation within 24 hours of birth.<\/jats:p><jats:p>Sciatic nerve section\/ligation produced 50% neuron death in L4 and L5 DRGs, but immunocytochemical methods showed that both SP\u2010immunoreactivity (\u2010IR) and CGRP\u2010IR recovered completely in dorsal horn. Radioimmunoassay confirmed that recovery of SP was not an artefact due to shrinkage. \u03b2\u2010Preprotachykinin (PPT)\u2010mRNA hybridization and SP\u2010IR were observed mostly in small neurons; \u03b1\u2010CGRP\u2010mRNA\u2010hybridized and CGRP\u2010IR neurons were more heterogeneous. The percentage of DRG neurons that contained SP (\u223c 25%) or CGRP (\u223c 50%) was the same in normal newborn and adult rats. Neither selective cell survival nor change in neuron phenotype was likely to contribute to the recovery seen in the dorsal horn, and DRG neurons ipsilateral to the lesion exhibited the same level of hybridized \u03b2\u2010PPT\u2010mRNA and \u03b1\u2010CGRP\u2010mRNA as intact DRG neurons. Because neither the constitutive level of expression of the genes nor peptide levels increased above those observed in intact DRG neurons, these mechanisms were also not responsible. Axotomized DRG neurons, however, contributed to recovery. Recovery was also due to sprouting by neurons in intact DRGs rostral and caudal to L4 and L5. \u00a9 1993 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/cne.903340304","type":"journal-article","created":{"date-parts":[[2005,1,1]],"date-time":"2005-01-01T08:57:59Z","timestamp":1104569879000},"page":"370-384","source":"Crossref","is-referenced-by-count":31,"title":["Restoration of substance P and calcitonin gene\u2010related peptide in dorsal root ganglia and dorsal horn after neonatal sciatic nerve lesion"],"prefix":"10.1002","volume":"334","author":[{"given":"Fatiha","family":"Nothias","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alan","family":"Tessler","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marion","family":"Murray","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,10,9]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0896-6273(91)90265-2"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01217921"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-0173(85)90006-2"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0169-328X(91)90100-C"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.901840208"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(81)90340-1"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(87)90616-7"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/0896-6273(91)90287-A"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4886(84)90190-0"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4612-4672-5_2"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-3806(83)90045-7"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.84.9.3060"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/0306-4522(90)90017-X"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.900940203"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.902620110"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3940(88)90781-1"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3940(91)90331-M"},{"key":"e_1_2_1_19_1","first-page":"1099","article-title":"Nerve growth factor regulates central terminals of primary sensory neurons","volume":"98","author":"Csillik B.","year":"1984","journal-title":"Z. 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