{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,16]],"date-time":"2026-01-16T06:52:35Z","timestamp":1768546355132,"version":"3.49.0"},"reference-count":61,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2004,10,10]],"date-time":"2004-10-10T00:00:00Z","timestamp":1097366400000},"content-version":"vor","delay-in-days":3436,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Comparative Neurology"],"published-print":{"date-parts":[[1995,5,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Glial cell\u2010lined derived neurotrophic factor (GDNF) has been shown to promote survival of developing mesencephalic dopaminergic neurons in vitro. In order to determine if there is a positive effect of GDNF on injured adult midbrain dopaminergic neurons in situ, we have carried out experiments in which a single dose of GDNF was injected into the substantia nigra following a unilateral lesion of the nigrostriatal system. Rats were unilaterally lesioned by a single stereotaxic injection of 6\u2010hydroxydopamine (6\u2010OHDA; 9 \u03bcg\/4 \u03bcl normal saline with 0.02% ascorbate) into the medial forebrain bundle and tested weekly for apomorphine\u2010induced (0.05 mg\/kg s. c. ) contralateral rotation behavior, Rats that manifested &gt;300 turns\/hour received a nigral injection of 100 \u03bcg GDNF, or cytochrome C as a control, 4 weeks following the 6\u2010OHDA lesion, Rotation behavior was quantified weekly for 5 weeks after GDNF. Rats were subsequently anesthetized, transcardially perfused, and processed for tyrosine hydroxylase immunohistochemistry. It was found that 100 \u03bcg GDNF decreased apomorphine\u2010induced rotational behavior by more than 85%. Immunohistochemical studies revealed that tyrosine hydroxylase immunoreactivity was equally reduced in the striatum ipsilateral to the lesion in both cytochrome C and GDNF\u2010injected animals. In contrast, large increments in tyrosine hydroxylase immunoreactivity were observed in the substantia nigra of animals treated with 100 \u03bcg of GDNF, with a significant increase in numbers of tyrosine hydroxylase\u2010immunoreactive cell bodies and neurites as well as a small increase in the cell body area of these neurons. The results suggest that GDNF can maintain the dopaminergic neuronal phenotype in a number of nigral neurons following a unilateral nigrostriatal lesion in the rat.<\/jats:p>","DOI":"10.1002\/cne.903550402","type":"journal-article","created":{"date-parts":[[2005,1,1]],"date-time":"2005-01-01T22:23:19Z","timestamp":1104618199000},"page":"479-489","source":"Crossref","is-referenced-by-count":244,"title":["Glial cell line\u2010derived neurotrophic factor supports survival of injured midbrain dopaminergic neurons"],"prefix":"10.1002","volume":"355","author":[{"given":"Kathryn E.","family":"Bowenkamp","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexander F.","family":"Hoffman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Greg A.","family":"Gerhardt","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael A.","family":"Henry","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul T.","family":"Biddle","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Barry J.","family":"Hoffer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ann\u2010Charlotte E.","family":"Granholm","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,10,10]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1748-1716.1983.tb07350.x"},{"key":"e_1_2_1_3_1","doi-asserted-by":"crossref","first-page":"264","DOI":"10.1111\/j.1600-0773.1966.tb00389.x","article-title":"Functional role of the nigroneostriatal dopamine neurons","volume":"24","author":"Anden N. 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