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Chromosomal translocations involving <jats:italic>KMT2A<\/jats:italic> often cause the formation of oncogenic fusion genes, such as the most common translocation t(4;11)(q21;q23) producing the KMT2A\u2010AFF1 chimera.<\/jats:p><\/jats:sec><jats:sec><jats:title>Aim<\/jats:title><jats:p>The aim of this study was to confirm and review the cytogenetic and molecular features of the <jats:italic>KMT2A<\/jats:italic>\u2010rearranged RS4;11 cell line and put those in context with other reports of cell lines also harbouring a t(4;11) rearrangement.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and Results<\/jats:title><jats:p>The main chromosomal rearrangements t(4;11)(q21;q23) and i(7q), described when the cell line was first established, were confirmed by fluorescence in situ hybridisation (FISH) and 24\u2010colour karyotyping by M\u2010FISH. Additional cytogenetic abnormalities were investigated by further FISH experiments, including the presence of trisomy 18 as a clonal abnormality and the discovery of one chromosome 8 being an i(8q), which indicates a duplication of the oncogene <jats:italic>MYC<\/jats:italic>. A homozygous deletion of 9p21 containing the tumour\u2010suppressor genes <jats:italic>CDKN2A<\/jats:italic> and <jats:italic>CDKN2B<\/jats:italic> was also revealed by FISH. The production of the fusion transcript <jats:italic>KMT2A\u2010AFF1<\/jats:italic> arising from the der(11)t(4;11) was confirmed by RT\u2010PCR, but sequencing of the amplified fragment revealed the presence of multiple isoforms. Two transcript variants, resulting from alternative splicing, were identified differing in one glutamine residue in the translated protein.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>As karyotype evolution is a common issue in cell lines, we highlight the need to monitor cell lines in order to re\u2010confirm their characteristics over time. We also reviewed the literature to provide a comparison of key features of several cell lines harbouring a t(4;11). This would guide scientists in selecting the most suitable research model for this particular type of <jats:italic>KMT2A<\/jats:italic>\u2010leukaemia.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cnr2.1207","type":"journal-article","created":{"date-parts":[[2019,8,7]],"date-time":"2019-08-07T13:55:31Z","timestamp":1565186131000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["The RS4;11 cell line as a model for leukaemia with t(4;11)(q21;q23): Revised characterisation of cytogenetic features"],"prefix":"10.1002","volume":"2","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0303-8683","authenticated-orcid":false,"given":"Denise","family":"Ragusa","sequence":"first","affiliation":[{"name":"Division of Biosciences, College of Health and Life Sciences, Institute of Environment, Health and Societies Brunel University London  Uxbridge UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Evgeny 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