{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T09:44:27Z","timestamp":1753868667526,"version":"3.41.2"},"reference-count":31,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2020,5,28]],"date-time":"2020-05-28T00:00:00Z","timestamp":1590624000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012446","name":"Akershus Universitetssykehus","doi-asserted-by":"publisher","award":["Division of Medicine"],"award-info":[{"award-number":["Division of Medicine"]}],"id":[{"id":"10.13039\/501100012446","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002794","name":"Cancerfonden","doi-asserted-by":"publisher","award":["4920\u2010B06\u201003PCD"],"award-info":[{"award-number":["4920\u2010B06\u201003PCD"]}],"id":[{"id":"10.13039\/501100002794","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006095","name":"Helse S\u00f8r-\u00d8st RHF","doi-asserted-by":"publisher","award":["2012056"],"award-info":[{"award-number":["2012056"]}],"id":[{"id":"10.13039\/501100006095","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100008730","name":"Kreftforeningen","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100008730","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Reports"],"published-print":{"date-parts":[[2020,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Ductal carcinoma in situ (DCIS) comprises a diverse group of preinvasive lesions in the breast and poses a considerable clinical challenge due to lack of markers of progression. Genomic alterations are to a large extent similar in DCIS and invasive carcinomas, although differences in copy number aberrations, gene expression patterns, and mutations exist. In mixed tumors with synchronous invasive breast cancer (IBC) and DCIS, it is still unclear to what extent invasive tumor cells are directly derived from the DCIS cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Aim<\/jats:title><jats:p>Our aim was to compare cancer\u2010relevant mutation profiles of different cellular compartments in mixed DCIS\/IBC and pure DCIS tumors.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and results<\/jats:title><jats:p>We performed targeted sequencing of 50 oncogenes in microdissected tissue from three different epithelial cell compartments (in situ, invasive, and normal adjacent epithelium) from 26 mixed breast carcinomas. In total, 44 tissue samples (19 invasive, 16 in situ, 9 normal) were subjected to sequencing using the Ion Torrent platform and the AmpliSeq Cancer Hotspot Panel v2. For comparison, 10 additional, pure DCIS lesions were sequenced.<\/jats:p><jats:p>Across all mixed samples, we detected 23 variants previously described in cancer. The most commonly affected genes were <jats:italic>TP53<\/jats:italic>, <jats:italic>PIK3CA<\/jats:italic>, and <jats:italic>ERBB2<\/jats:italic>. The <jats:italic>PIK3CA<\/jats:italic>:p.H1047R variant was found in nine samples from six patients. Most variants detected in invasive compartments were also found in the corresponding in situ cell compartment indicating a clonal relationship between the tumor stages. A lower frequency of variants were observed in pure DCIS lesions.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Similar mutation profiles between in situ and invasive cell compartments indicate a similar origin of the two tumor stages in mixed breast tumors. The lower number of potential driver variants found in pure DCIS compared with the in situ cell compartments of mixed tumors may imply that pure DCIS is captured earlier in the path of progression to invasive disease.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cnr2.1248","type":"journal-article","created":{"date-parts":[[2020,5,28]],"date-time":"2020-05-28T14:34:18Z","timestamp":1590676458000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Comparable cancer\u2010relevant mutation profiles in synchronous ductal carcinoma in situ and invasive breast cancer"],"prefix":"10.1002","volume":"3","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0999-1106","authenticated-orcid":false,"given":"Helga","family":"Bergholtz","sequence":"first","affiliation":[{"name":"Department of Cancer Genetics, Institute for Cancer Research Oslo University Hospital  Oslo Norway"},{"name":"Institute of Clinical Medicine, Faculty of Medicine University of Oslo  Oslo Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Surendra","family":"Kumar","sequence":"additional","affiliation":[{"name":"Department of Cancer Genetics, Institute for Cancer Research Oslo University Hospital  Oslo Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fredrik","family":"W\u00e4rnberg","sequence":"additional","affiliation":[{"name":"Department of Surgical Sciences Uppsala University  Uppsala Sweden"},{"name":"Department of Surgery Uppsala Academic Hospital  Uppsala Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5176-7808","authenticated-orcid":false,"given":"Torben","family":"L\u00fcders","sequence":"additional","affiliation":[{"name":"Institute of Clinical Medicine, Faculty of Medicine University of Oslo  Oslo Norway"},{"name":"Department of Clinical Molecular Biology (EpiGen), Division of Medicine Akershus University Hospital  L\u00f8renskog Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vessela","family":"Kristensen","sequence":"additional","affiliation":[{"name":"Department of Cancer Genetics, Institute for Cancer Research Oslo University Hospital  Oslo Norway"},{"name":"Institute of Clinical Medicine, Faculty of Medicine University of Oslo  Oslo Norway"},{"name":"Department of Clinical Molecular Biology (EpiGen), Division of Medicine Akershus University Hospital  L\u00f8renskog Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5995-2319","authenticated-orcid":false,"given":"Therese","family":"S\u00f8rlie","sequence":"additional","affiliation":[{"name":"Department of Cancer Genetics, Institute for Cancer Research Oslo University Hospital  Oslo Norway"},{"name":"Institute of Clinical Medicine, Faculty of Medicine University of Oslo  Oslo Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,5,28]]},"reference":[{"key":"e_1_2_11_2_1","series-title":"WHO Classification of Tumours","volume-title":"WHO Classification of Tumours of the Breast","author":"Lakhani S","year":"2012"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21321"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10549-005-9101-z"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/modpathol.2014.141"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2017.00248"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molonc.2013.07.005"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10549-015-3509-x"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molonc.2009.03.001"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/path.3990"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-08-2660"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-13-2267"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2008.18.3103"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjc.6605655"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13277-012-0602-1"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/path.5262"},{"key":"e_1_2_11_17_1","first-page":"167","article-title":"Ductal carcinoma in situ: a proposal for a new classification","volume":"11","author":"Holland R","year":"1994","journal-title":"Semin Diagn Pathol"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.46.2.189-b"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1054\/bjoc.2001.1995"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.1754"},{"key":"e_1_2_11_21_1","article-title":"Variation across 141,456 human exomes and genomes reveals the spectrum of loss\u2010of\u2010function intolerance across human protein\u2010coding genes","volume":"531210","author":"Karczewski KJ","year":"2019","journal-title":"bioRxiv"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep06255"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature17676"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472-CAN-04-3913"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.3162"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1097\/PAI.0b013e3181acaded"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1530\/ERC-19-0019"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/modpathol.2017.21"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00428-018-2463-5"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10549-009-0406-1"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexmp.2009.09.016"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pcbi.1003031"}],"container-title":["Cancer Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcnr2.1248","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cnr2.1248","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/cnr2.1248","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cnr2.1248","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,6]],"date-time":"2023-09-06T04:53:19Z","timestamp":1693975999000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cnr2.1248"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,5,28]]},"references-count":31,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2020,6]]}},"alternative-id":["10.1002\/cnr2.1248"],"URL":"https:\/\/doi.org\/10.1002\/cnr2.1248","archive":["Portico"],"relation":{},"ISSN":["2573-8348","2573-8348"],"issn-type":[{"type":"print","value":"2573-8348"},{"type":"electronic","value":"2573-8348"}],"subject":[],"published":{"date-parts":[[2020,5,28]]},"assertion":[{"value":"2019-10-21","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-05-05","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-05-28","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e1248"}}