{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,26]],"date-time":"2026-10-26T23:20:10Z","timestamp":1793056810503,"version":"4.5.0"},"reference-count":42,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2022,6,12]],"date-time":"2022-06-12T00:00:00Z","timestamp":1654992000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000646","name":"Japan Society for the Promotion of Science London","doi-asserted-by":"publisher","award":["19K09166"],"award-info":[{"award-number":["19K09166"]}],"id":[{"id":"10.13039\/501100000646","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Reports"],"published-print":{"date-parts":[[2023,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Peritoneal metastasis (PM) is one of the most common causes of noncurative surgery and the most frequent recurrence pattern in gastric cancer (GC). During the process of PM, GC cells detached from primary tumor interact with human peritoneal mesothelial cells (HPMC) overlapped with adipose tissues such as the omentum or mesentery. Although the interaction with HPMC promotes the malignancy of GC, the role of adipose tissues remains unclear.<\/jats:p><\/jats:sec><jats:sec><jats:title>Aims<\/jats:title><jats:p>We aimed to clarify how adipose tissue are affected by adjacent primary tumors during the expression of adipokines and to elucidate whether GC cells transform adipocytes into CAFs in vitro. In addition, we investigated whether GC cells are affected by adipocytes in their ability to infiltrate.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We investigated the phenotypic conversion of adipocytes during the malignant process of GC cells in vivo and in vitro. We evaluated the expression levels of adiponectin in the omental adipose tissue of gastric cancer patients by western blotting. Following adipocytes\/gastric cancer cells coculture, adipocyte markers, adiponectin receptors, and inflammatory cytokine markers were detected by real\u2010time PCR and\/or western blotting in the single\u2010cultured and co\u2010cultured adipocytes; cancer\u2010associated fibroblast (CAF) markers were detected by immunofluorescence and western blotting in the single\u2010cultured and co\u2010cultured adipocytes; invasion assays were performed in single cultured and co\u2010cultured MKN45 and OCUM.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In omental adipose tissues that are situated close to the primary tumors, the expression of adiponectin tended to decrease in patients with subserosal or serosal invasion. By co\u2010culturing with GC cells, adipocytes were dedifferentiated and the expression levels of CAF marker FSP1 and inflammatory cytokines, <jats:italic>PAI\u20101<\/jats:italic> and <jats:italic>IL\u20106<\/jats:italic>, significantly increased (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05). Furthermore, GC cells co\u2010cultured with adipocytes showed enhanced invasion ability.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Our findings suggest that the phenotypic conversion of adipocytes may promote the malignancy of GC in the construction of the cancer microenvironment of PM.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cnr2.1647","type":"journal-article","created":{"date-parts":[[2022,6,13]],"date-time":"2022-06-13T00:29:22Z","timestamp":1655080162000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Adipocytes contribute to tumor progression and invasion of peritoneal metastasis by interacting with gastric cancer cells as cancer associated fibroblasts"],"prefix":"10.1002","volume":"6","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9075-9982","authenticated-orcid":false,"given":"Toshihide","family":"Hamabe\u2010Horiike","sequence":"first","affiliation":[{"name":"Center for Biomedical Research and Education, School of Medicine Kanazawa University  Kanazawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shin\u2010ichi","family":"Harada","sequence":"additional","affiliation":[{"name":"Center for Biomedical Research and Education, School of Medicine Kanazawa University  Kanazawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kyoko","family":"Yoshida","sequence":"additional","affiliation":[{"name":"Department of Gastroenterological Surgery, Division of Cancer Medicine, Graduate School of Medical Science Kanazawa University  Kanazawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Kinoshita","sequence":"additional","affiliation":[{"name":"Department of Gastroenterological Surgery, Division of Cancer Medicine, Graduate School of Medical Science Kanazawa University  Kanazawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8086-739X","authenticated-orcid":false,"given":"Takahisa","family":"Yamaguchi","sequence":"additional","affiliation":[{"name":"Department of Gastroenterological Surgery, Division of Cancer Medicine, Graduate School of Medical Science Kanazawa University  Kanazawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sachio","family":"Fushida","sequence":"additional","affiliation":[{"name":"Department of Gastroenterological Surgery, Division of Cancer Medicine, Graduate School of Medical Science Kanazawa University  Kanazawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,6,12]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/jjco\/hyt166"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/annonc\/mdp260"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00280\u2010013\u20102122\u20100"},{"key":"e_1_2_10_5_1","doi-asserted-by":"crossref","unstructured":"SugarbakerPH.Peritonectomy procedures. Vol 221 1995.","DOI":"10.1097\/00000658-199501000-00004"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/bjs.4695"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0039\u20106060(96)80145\u20100"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1245\/s10434\u2010011\u20101631\u20105"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.3892\/ijo.2012.1490"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10120\u2010017\u20100726\u20105"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10120\u2010015\u20100579\u20108"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10120\u2010014\u20100380\u20100"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2014.09.019"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10120\u2010020\u201001092\u20102"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2013.08.073"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.2147\/CMAR.S167846"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1080\/07357900701684057"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0215(19971127)73:5<656::AID-IJC8>3.0.CO;2-W"},{"issue":"11","key":"e_1_2_10_19_1","first-page":"4751","article-title":"Expression of CXCR4 and its ligand SDF\u20101 in intestinal\u2010type gastric cancer is associated with lymph node and liver metastasis","volume":"29","author":"Iwasa S","year":"2009","journal-title":"Anticancer Res"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.3892\/ol.2017.7149"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocel.2016.12.002"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00441\u2010011\u20101144\u20103"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1440\u20101827.1986.tb01461.x"},{"issue":"9","key":"e_1_2_10_24_1","first-page":"849","article-title":"Detection of amplified DNA sequences in gastric cancers by a DNA renaturation method in gel","volume":"77","author":"Nakatani H","year":"1986","journal-title":"Jpn J Cancer Res"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.1995.486"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00157685"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471\u20102199\u20107\u20103"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008\u20105472.CAN\u201010\u20103323"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008\u20105472.CAN\u201013\u20100530"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.25031"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1186\/1756\u20109966\u201030\u2010107"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1349\u20107006.2007.00486.x"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.3892\/ol.2016.4498"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2018.01.153"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.114.21.3905"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep35483"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1007\/bf03401666"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2005030257"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.2337\/db13\u20100436"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1002\/cphy.c160022"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.ijo.0800606"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.7150\/thno.36037"}],"container-title":["Cancer Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cnr2.1647","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/cnr2.1647","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cnr2.1647","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,18]],"date-time":"2023-08-18T01:59:30Z","timestamp":1692323970000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cnr2.1647"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,6,12]]},"references-count":42,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2023,1]]}},"alternative-id":["10.1002\/cnr2.1647"],"URL":"https:\/\/doi.org\/10.1002\/cnr2.1647","archive":["Portico"],"relation":{},"ISSN":["2573-8348","2573-8348"],"issn-type":[{"value":"2573-8348","type":"print"},{"value":"2573-8348","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,6,12]]},"assertion":[{"value":"2021-12-28","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-04-17","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-06-12","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e1647"}}