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Radiation effects on tumor stromal components like CAFs in the context of radiation treatment is not well\u2010described.<\/jats:p><\/jats:sec><jats:sec><jats:title>Aim<\/jats:title><jats:p>This study explores potential changes induced by ionizing radiation (IR) on platelet\u2010derived growth factor (PDGF)\/PDGFRs and transforming growth factor\u2010beta (TGF\u2010\u03b2)\/TGF\u03b2Rs signaling systems in CAFs.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and Results<\/jats:title><jats:p>Experiments were carried out by employing primary cultures of human CAFs isolated from freshly resected non\u2010small cell lung carcinoma tumor tissues. CAF cultures from nine donors were treated with one high (1\u2009\u00d7\u200918\u2009Gy) or three fractionated (3\u2009\u00d7\u20096\u2009Gy) radiation doses. Alterations in expression levels of TGF\u03b2RII and PDGFR\u03b1\/\u03b2 induced by IR were analyzed by western blots and flow cytometry. In the presence or absence of cognate ligands, receptor activation was studied in nonirradiated and irradiated CAFs. Radiation exposure did not exert changes in expression of PDGF or TGF\u2010\u03b2 receptors in CAFs. Additionally, IR alone was unable to trigger activation of either receptor. The radiation regimens tested did not affect PDGFR\u03b2 signaling in the presence of PDGF\u2010BB. In contrast, signaling via pSmad2\/3 and pSmad1\/5\/8 appeared to be down\u2010regulated in irradiated CAFs after stimulation with TGF\u2010\u03b2, as compared with controls.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Our data demonstrate that IR by itself is insufficient to induce measurable changes in PDGF or TGF\u2010\u03b2 receptor expression levels or to induce receptor activation in CAFs. However, in the presence of their respective ligands, exposure to radiation at certain doses appear to interfere with TGF\u2010\u03b2 receptor signaling.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cnr2.2018","type":"journal-article","created":{"date-parts":[[2024,3,16]],"date-time":"2024-03-16T06:49:33Z","timestamp":1710571773000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Radiation\u2010induced effects on TGF\u2010\u03b2 and PDGF receptor signaling in cancer\u2010associated fibroblasts"],"prefix":"10.1002","volume":"7","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0919-3035","authenticated-orcid":false,"given":"Nannan","family":"Yang","sequence":"first","affiliation":[{"name":"Department of Community Medicine, Faculty of Health Sciences UiT The Arctic University of Norway  Troms\u00f8 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