{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T09:44:32Z","timestamp":1753868672620,"version":"3.41.2"},"reference-count":20,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2024,11,14]],"date-time":"2024-11-14T00:00:00Z","timestamp":1731542400000},"content-version":"vor","delay-in-days":13,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100015468","name":"Kihara Memorial Yokohama Foundation","doi-asserted-by":"publisher","award":["2022,1046\u201028"],"award-info":[{"award-number":["2022,1046\u201028"]}],"id":[{"id":"10.13039\/100015468","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Reports"],"published-print":{"date-parts":[[2024,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Recent advances in cancer genome analysis and the practice of precision medicine have made it possible to identify fractions with rare genetic alterations. Among biliary tract cancers, <jats:italic>EGFR<\/jats:italic>\u2010amplified cancers are known to be rare fractions across organs and have a poor prognosis. The use of anti\u2010EGFR antibody for <jats:italic>EGFR<\/jats:italic>\u2010amplified cancers has been promising; however, the evidence is not yet clear.<\/jats:p><\/jats:sec><jats:sec><jats:title>Case<\/jats:title><jats:p>In this report, we describe the case of a 48\u2010year\u2010old man diagnosed with advanced gallbladder cancer. The patient was administered gemcitabine plus cisplatin, followed by S\u20101 monotherapy; however, disease progression was observed after two cycles of each regimen. Comprehensive genomic profiling test revealed <jats:italic>EGFR<\/jats:italic>\u2010amplification, and the patient was treated with combination therapy with the anti\u2010EGFR antibody necitumumab, gemcitabine, and cisplatin. After two cycles of treatment, tumor size reduced, and the treatment response was evaluated as partial response. On Day 90, after five cycles of treatment, tumor progression was confirmed. In addition, after disease progression, liquid biopsy revealed acquired pathogenic gene alterations suggesting anti\u2010EGFR antibody resistance.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>This report supports the clinical benefit of anti\u2010EGFR antibodies for <jats:italic>EGFR<\/jats:italic>\u2010amplified biliary tract cancers and the importance of genomic analysis in personalized therapy and drug resistance research.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cnr2.70053","type":"journal-article","created":{"date-parts":[[2024,11,14]],"date-time":"2024-11-14T12:46:31Z","timestamp":1731588391000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A Case of Advanced Biliary Tract Cancer With <i>EGFR<\/i> Amplification That Responded to Necitumumab"],"prefix":"10.1002","volume":"7","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5272-5167","authenticated-orcid":false,"given":"Makoto","family":"Sugimori","sequence":"first","affiliation":[{"name":"Division of Cancer Genome Medicine Yokohama City University Medical Center  Yokohama Japan"},{"name":"Gastroenterological Center Yokohama City University Medical Center  Yokohama Japan"},{"name":"Division of Genomics Laboratory Yokohama City University Medical Center  Yokohama Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masaki","family":"Nishimura","sequence":"additional","affiliation":[{"name":"Gastroenterological Center Yokohama City University Medical Center  Yokohama Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazuya","family":"Sugimori","sequence":"additional","affiliation":[{"name":"Gastroenterological Center Yokohama City University Medical Center  Yokohama 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