{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T09:44:39Z","timestamp":1753868679222,"version":"3.41.2"},"reference-count":36,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2025,4,13]],"date-time":"2025-04-13T00:00:00Z","timestamp":1744502400000},"content-version":"vor","delay-in-days":12,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81270842","81470985"],"award-info":[{"award-number":["81270842","81470985"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Reports"],"published-print":{"date-parts":[[2025,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Objectives<\/jats:title><jats:p>The primary aim of this research is to identify and describe the distinct patterns of microRNAs (miRNAs) that are unusually expressed in benign prostatic hyperplasia (BPH) tissues compared to normal prostatic tissues.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>The investigation began with the collection of three samples each from normal prostatic and BPH tissues. These samples underwent miRNA microarray analysis using the Agilent platform. Following the preliminary screening, a larger sample set, comprising five normal prostatic tissues and 36 BPH tissues, was subjected to qRT\u2010PCR to confirm the differential expression of the miRNAs initially identified.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The microarray analysis revealed that only miR\u2010126\u20103p and miR\u20104672 exhibited an expression profile marked by both a fold change &gt;\u20091.5 and <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05, indicating significant downregulation in BPH tissues. MiR\u2010145\u20103p and miR\u2010143\u20103p also showed downregulation with fold changes greater than 1.5; however, these changes did not reach statistical significance as their p\u2010values were above 0.05. Further attempts to validate these findings through qRT\u2010PCR did not confirm any notable dysregulation among the four miRNAs studied; the variations in their expression levels between normal and BPH tissues did not achieve statistical significance, with p\u2010values exceeding 0.1. From the data accrued, it can be inferred that the roles of miR\u20104672, miR\u2010126\u20103p, miR\u2010145\u20103p, and miR\u2010143\u20103p in BPH development continue to be an unresolved mystery, and the need for further investigation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>This preliminary investigation establishes a foundation for subsequent studies aimed at elucidating the regulatory mechanisms underlying BPH. However, these results highlight the need for further investigation employing a more extensive sample size and comprehensive clinical data to elucidate their potential roles in the pathogenesis of BPH.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cnr2.70178","type":"journal-article","created":{"date-parts":[[2025,4,14]],"date-time":"2025-04-14T04:18:46Z","timestamp":1744604326000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Identification and Characterization of Differentially Expressed <scp>MicroRNAs<\/scp> in Benign Prostatic Hyperplasia"],"prefix":"10.1002","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0515-7317","authenticated-orcid":false,"given":"Lingmin","family":"Song","sequence":"first","affiliation":[{"name":"Department of Urologic Surgery Ningbo Yinzhou No. 2 Hospital  Ningbo China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-3062-2103","authenticated-orcid":false,"given":"Xue","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Urologic Surgery Ningbo Yinzhou No. 2 Hospital  Ningbo China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gang","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Urologic Surgery Ningbo Yinzhou No. 2 Hospital  Ningbo China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liwei","family":"Zheng","sequence":"additional","affiliation":[{"name":"Department of Urologic Surgery Ningbo Yinzhou No. 2 Hospital  Ningbo China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhansong","family":"Zhou","sequence":"additional","affiliation":[{"name":"Urological Research Institute of People's Liberation Army, Southwest Hospital, Army Medical University  Chongqing China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,4,13]]},"reference":[{"doi-asserted-by":"publisher","key":"e_1_2_11_2_1","DOI":"10.3390\/ijms241411634"},{"doi-asserted-by":"publisher","key":"e_1_2_11_3_1","DOI":"10.3892\/mmr.2017.8318"},{"doi-asserted-by":"publisher","key":"e_1_2_11_4_1","DOI":"10.3390\/ijms24032870"},{"doi-asserted-by":"publisher","key":"e_1_2_11_5_1","DOI":"10.3390\/cells12020306"},{"doi-asserted-by":"publisher","key":"e_1_2_11_6_1","DOI":"10.1186\/1471-2164-10-407"},{"doi-asserted-by":"publisher","key":"e_1_2_11_7_1","DOI":"10.1111\/iju.15043"},{"doi-asserted-by":"publisher","key":"e_1_2_11_8_1","DOI":"10.1007\/s11010-023-04854-5"},{"doi-asserted-by":"publisher","key":"e_1_2_11_9_1","DOI":"10.1038\/s41388-024-03057-6"},{"issue":"5","key":"e_1_2_11_10_1","first-page":"675","article-title":"MiR\u20101202 Regulates BPH\u20101 Cell Proliferation, Apoptosis, and Epithelial\u2010To\u2010Mesenchymal Transition Through Targeting HMGCL","volume":"56","author":"Wang Z.","year":"2024","journal-title":"Acta Biochimica et Biophysica Sinica Shanghai"},{"doi-asserted-by":"publisher","key":"e_1_2_11_11_1","DOI":"10.1002\/jbt.23391"},{"doi-asserted-by":"publisher","key":"e_1_2_11_12_1","DOI":"10.1038\/s41420-023-01696-4"},{"doi-asserted-by":"publisher","key":"e_1_2_11_13_1","DOI":"10.1016\/j.ejphar.2023.175993"},{"doi-asserted-by":"publisher","key":"e_1_2_11_14_1","DOI":"10.18632\/aging.102395"},{"doi-asserted-by":"publisher","key":"e_1_2_11_15_1","DOI":"10.1007\/s11427-011-4149-y"},{"doi-asserted-by":"publisher","key":"e_1_2_11_16_1","DOI":"10.1016\/j.trsl.2021.11.010"},{"doi-asserted-by":"publisher","key":"e_1_2_11_17_1","DOI":"10.7150\/ijms.26329"},{"doi-asserted-by":"publisher","key":"e_1_2_11_18_1","DOI":"10.5301\/jbm.5000069"},{"doi-asserted-by":"publisher","key":"e_1_2_11_19_1","DOI":"10.3390\/genes13010075"},{"doi-asserted-by":"publisher","key":"e_1_2_11_20_1","DOI":"10.1097\/MD.0000000000020904"},{"doi-asserted-by":"publisher","key":"e_1_2_11_21_1","DOI":"10.1186\/s12957-023-03151-1"},{"doi-asserted-by":"publisher","key":"e_1_2_11_22_1","DOI":"10.15283\/ijsc21170"},{"doi-asserted-by":"publisher","key":"e_1_2_11_23_1","DOI":"10.1159\/000486058"},{"doi-asserted-by":"publisher","key":"e_1_2_11_24_1","DOI":"10.1016\/j.ejca.2019.06.006"},{"doi-asserted-by":"publisher","key":"e_1_2_11_25_1","DOI":"10.1530\/REP-14-0095"},{"doi-asserted-by":"publisher","key":"e_1_2_11_26_1","DOI":"10.1007\/s13105-024-01017-y"},{"doi-asserted-by":"publisher","key":"e_1_2_11_27_1","DOI":"10.26355\/eurrev_202209_29632"},{"doi-asserted-by":"publisher","key":"e_1_2_11_28_1","DOI":"10.1002\/wsbm.1208"},{"doi-asserted-by":"publisher","key":"e_1_2_11_29_1","DOI":"10.1186\/s12967-014-0259-1"},{"doi-asserted-by":"publisher","key":"e_1_2_11_30_1","DOI":"10.21037\/tau-20-421"},{"key":"e_1_2_11_31_1","first-page":"169","article-title":"Urinary Extracellular Vesicle\u2010Derived MiR\u2010126\u20103p Predicts Lymph Node Invasion in Patients With High\u2010Risk Prostate Cancer","author":"Dong L.","year":"2024","journal-title":"Research Square"},{"doi-asserted-by":"publisher","key":"e_1_2_11_32_1","DOI":"10.1038\/onc.2015.295"},{"doi-asserted-by":"publisher","key":"e_1_2_11_33_1","DOI":"10.3892\/ijo.2019.4782"},{"doi-asserted-by":"publisher","key":"e_1_2_11_34_1","DOI":"10.1038\/bjc.2017.191"},{"doi-asserted-by":"publisher","key":"e_1_2_11_35_1","DOI":"10.3390\/cells12182207"},{"doi-asserted-by":"publisher","key":"e_1_2_11_36_1","DOI":"10.1016\/j.mcp.2023.101901"},{"doi-asserted-by":"publisher","key":"e_1_2_11_37_1","DOI":"10.1093\/nar\/gku461"}],"container-title":["Cancer Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cnr2.70178","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,29]],"date-time":"2025-04-29T11:06:40Z","timestamp":1745924800000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cnr2.70178"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,4]]},"references-count":36,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2025,4]]}},"alternative-id":["10.1002\/cnr2.70178"],"URL":"https:\/\/doi.org\/10.1002\/cnr2.70178","archive":["Portico"],"relation":{},"ISSN":["2573-8348","2573-8348"],"issn-type":[{"type":"print","value":"2573-8348"},{"type":"electronic","value":"2573-8348"}],"subject":[],"published":{"date-parts":[[2025,4]]},"assertion":[{"value":"2024-07-08","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-02-25","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-04-13","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70178"}}