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However, it remains uncertain whether APOBEC3B\u2010induced mutations accelerate tumor progression or not. In this paper, we established a mouse model with A3B overexpression and investigated whether the introduction of A3B overexpression accelerates tumor development in <jats:italic>Tp53<\/jats:italic> hemizygous mice.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We established A3B transgenic mouse by microinjection and selected the mouse which has only one A3B transgene by genomic qPCR and southern blotting using the probe against the transgene. A3B expression was validated by qPCR, immunoblotting, immunohistochemistry and <jats:italic>in vitro<\/jats:italic> CDA assays using lysates of this transgenic mouse liver, spleen and bone marrow. We interbreed this transgenic mouse model with CAG\u2010Cre and <jats:italic>Tp53<\/jats:italic> knockout mice and observed differences in tumor progression and survival between <jats:italic>Tp53<\/jats:italic> hemizygous mice and <jats:italic>Tp53<\/jats:italic> homozygous mice irrespective of A3B expression. Finally, comprehensive genomic mutation analysis was done using the developed tumors.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We established A3B transgenic mouse which has only one transgene. A3B expression and its CDA activity were confirmed in liver cells and tumor tissues of mice overexpressing A3B. <jats:italic>Tp53<\/jats:italic> hemizygous mice developed osteosarcomas, spindle and pleomorphic sarcomas, and squamous cell carcinomas, however we did not observe any difference in tumor development between the mice with or without A3B expression. The tumor with A3B expression has more high\u2010VAF mutations than the one without A3B, but these mutations are not APOBEC signature.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>We developed a Cre inducible A3B transgenic mouse model bearing single copy of <jats:italic>A3B<\/jats:italic> gene. Although the introduction of A3B overexpression did not accelerate tumor development in <jats:italic>Tp53<\/jats:italic> hemizygous mice, our mouse model with A3B overexpression is well\u2010validated and useful for further research.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cnr2.70189","type":"journal-article","created":{"date-parts":[[2025,4,11]],"date-time":"2025-04-11T11:08:50Z","timestamp":1744369730000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["<scp>APOBEC3B<\/scp> Does Not Promote Tumor Progression in <i>Tp53<\/i> Hemizygous Mice"],"prefix":"10.1002","volume":"8","author":[{"given":"Yoshihito","family":"Horisawa","sequence":"first","affiliation":[{"name":"Department of Hematology Graduate School of Medicine, Kyoto University  Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tadahiko","family":"Matsumoto","sequence":"additional","affiliation":[{"name":"Department of Hematology 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