{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T16:21:10Z","timestamp":1773332470017,"version":"3.50.1"},"reference-count":31,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2025,8,3]],"date-time":"2025-08-03T00:00:00Z","timestamp":1754179200000},"content-version":"vor","delay-in-days":2,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Reports"],"published-print":{"date-parts":[[2025,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Microribonucleic acid (MicroRNAs\/miRNAs) play a significant role in cancer progression by changing cellular functions through the modulation of protein expressions. The potential of different miRNAs to alter the expression of the mammalian target of rapamycin (<jats:italic>mTOR<\/jats:italic>)\/protein kinase B (<jats:italic>PKB<\/jats:italic> or <jats:italic>AKT<\/jats:italic>)\/phosphatidylinositol\u20103\u2010kinase (<jats:italic>PI3K<\/jats:italic>) signaling cascade, a key pathway in the progression of acute myeloid leukemia (AML), has been demonstrated across different types of cancers.<\/jats:p><\/jats:sec><jats:sec><jats:title>Aims<\/jats:title><jats:p>This study aims to explore the effects of <jats:italic>miR\u2010100<\/jats:italic> and <jats:italic>miR\u2010101<\/jats:italic> on the <jats:italic>mTOR\/AKT\/PI3K<\/jats:italic> signaling pathway in AML.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and Results<\/jats:title><jats:p>Initially, we employed the TargetScan, miRDB, and miRanda databases to identify the target proteins of <jats:italic>miR\u2010100<\/jats:italic> and <jats:italic>miR\u2010101<\/jats:italic>. Following a comprehensive analysis, we identified the <jats:italic>mTOR<\/jats:italic>\/<jats:italic>AKT<\/jats:italic>\/<jats:italic>PI3K<\/jats:italic> signaling pathway as a significant target for investigation in patients with AML. In this case\u2013control study, the expression levels of miRNAs and genes were analyzed in 21 AML patients and 9 healthy controls using quantitative reverse transcription polymerase chain reaction (qRT\u2010PCR). The results showed that <jats:italic>miR\u2010100<\/jats:italic> was significantly upregulated, while <jats:italic>miR\u2010101<\/jats:italic>, <jats:italic>mTOR<\/jats:italic>, and <jats:italic>PI3K<\/jats:italic> were downregulated in AML patients. Correlation analysis revealed a negative relationship for <jats:italic>miR\u2010100<\/jats:italic> and a positive one for <jats:italic>miR\u2010101<\/jats:italic> with <jats:italic>mTOR<\/jats:italic>, but no significant correlation with <jats:italic>AKT1<\/jats:italic> and <jats:italic>PI3K<\/jats:italic> genes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>These findings suggest that both <jats:italic>miR\u2010100<\/jats:italic> and <jats:italic>miR\u2010101<\/jats:italic> act as tumor suppressors via the <jats:italic>mTOR\/AKT\/PI3K<\/jats:italic> signaling pathway, highlighting their potential as therapeutic targets in AML.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cnr2.70264","type":"journal-article","created":{"date-parts":[[2025,8,4]],"date-time":"2025-08-04T03:43:26Z","timestamp":1754279006000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Targeting the <scp>mTOR<\/scp> Signaling Pathway Through <scp>miR\u2010100<\/scp> and <scp>miR\u2010101<\/scp> in De Novo Acute Myeloid Leukemia: Implications for Therapeutic Intervention"],"prefix":"10.1002","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7922-7942","authenticated-orcid":false,"given":"Maryam","family":"Kargar","sequence":"first","affiliation":[{"name":"Department of Laboratory Hematology and Blood Bank, School of Allied Medical Science Shahid Beheshti University of Medical Sciences  Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mehdi","family":"Allahbakhshian Farsani","sequence":"additional","affiliation":[{"name":"Department of Laboratory Hematology and Blood Bank, School of Allied Medical Science Shahid Beheshti University of Medical Sciences  Tehran Iran"},{"name":"HSCT Research Center, Laboratory Hematology and Blood Banking Department, School of Allied Medical Sciences Shahid Beheshti University of Medical Sciences  Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Javad","family":"Garavand","sequence":"additional","affiliation":[{"name":"Department of Laboratory Sciences, School of Allied Medical Sciences Ahvaz Jundishapur University of Medical Sciences  Ahvaz Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mahnaz","family":"Gorji","sequence":"additional","affiliation":[{"name":"Department of Laboratory Hematology and Blood Bank, School of Allied Medical Science Shahid Beheshti University of Medical Sciences  Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohammad","family":"Rafiee","sequence":"additional","affiliation":[{"name":"Department of Medical Laboratory Sciences, School of Para Medicine Hamadan University of Medical Sciences  Hamadan Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seyed Sobhan","family":"Bahreiny","sequence":"additional","affiliation":[{"name":"Medical Basic Sciences Research Institute, Physiology Research Center, Department of Physiology, School of Medicine Ahvaz Jundishapur University of Medical Sciences  Ahvaz Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohammad Hossein","family":"Mohammadi","sequence":"additional","affiliation":[{"name":"HSCT Research Center, Laboratory Hematology and Blood Banking Department, School of Allied Medical Sciences Shahid Beheshti University of Medical Sciences  Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,8,3]]},"reference":[{"key":"e_1_2_13_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/onc.2011.208"},{"key":"e_1_2_13_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00277-019-03887-5"},{"issue":"10","key":"e_1_2_13_4_1","first-page":"1934","article-title":"Treatment of Acute Myeloid Leukemia Under Relapsed\/Refractory Conditions or in Older Adults","volume":"57","author":"Yamauchi T.","year":"2016","journal-title":"[Rinsh\u014d Ketsueki] Japanese Journal of Clinical Hematology"},{"key":"e_1_2_13_5_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.12343"},{"key":"e_1_2_13_6_1","first-page":"213","article-title":"Upregulation of microRNA\u2010100 Predicts Poor Prognosis in Patients With Pediatric Acute Myeloid Leukemia","volume":"5","author":"Bai J.","year":"2012","journal-title":"Oncotargets and Therapy"},{"key":"e_1_2_13_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2019.11.156"},{"key":"e_1_2_13_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11596-014-1369-y"},{"key":"e_1_2_13_9_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2018.209437"},{"key":"e_1_2_13_10_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms19020382"},{"key":"e_1_2_13_11_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-10-4058"},{"key":"e_1_2_13_12_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms14023874"},{"key":"e_1_2_13_13_1","doi-asserted-by":"publisher","DOI":"10.1158\/1535-7163.MCT-12-0273"},{"key":"e_1_2_13_14_1","doi-asserted-by":"publisher","DOI":"10.3233\/CBM-170620"},{"key":"e_1_2_13_15_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0115293"},{"key":"e_1_2_13_16_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2013-01-480079"},{"key":"e_1_2_13_17_1","doi-asserted-by":"publisher","DOI":"10.17219\/acem\/144170"},{"key":"e_1_2_13_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-319-03725-7_9"},{"key":"e_1_2_13_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.29333"},{"key":"e_1_2_13_20_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.25541"},{"key":"e_1_2_13_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.2013.562"},{"key":"e_1_2_13_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clinre.2019.02.003"},{"key":"e_1_2_13_23_1","doi-asserted-by":"publisher","DOI":"10.3390\/jcm9092934"},{"key":"e_1_2_13_24_1","doi-asserted-by":"publisher","DOI":"10.1177\/1533033818758772"},{"key":"e_1_2_13_25_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12943-023-01820-z"},{"key":"e_1_2_13_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep17499"},{"issue":"4","key":"e_1_2_13_27_1","first-page":"291","article-title":"Impact of mTOR Expression on Clinical Outcome in Paediatric Patients With B\u2010Cell Acute Lymphoblastic Leukaemia \u2010 Preliminary Report","volume":"20","author":"Uli\u0144ska E.","year":"2016","journal-title":"Contemp Oncol (Pozn)"},{"issue":"4","key":"e_1_2_13_28_1","first-page":"5236","article-title":"Expression of Phosphorylated Akt\/mTOR and Clinical Significance in Human Ameloblastoma","volume":"8","author":"Li N.","year":"2015","journal-title":"International Journal of Clinical and Experimental Medicine"},{"issue":"2","key":"e_1_2_13_29_1","first-page":"158","article-title":"AKT Expression Is Associated With Degree of Pathologic Response in Adenocarcinoma of the Esophagus Treated With Neoadjuvant Therapy","volume":"7","author":"Saeed N.","year":"2015","journal-title":"Journal of Gastrointestinal Oncology"},{"key":"e_1_2_13_30_1","doi-asserted-by":"publisher","DOI":"10.3892\/ol.2013.1132"},{"key":"e_1_2_13_31_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12253-016-0066-5"},{"key":"e_1_2_13_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.drup.2007.11.003"}],"container-title":["Cancer Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cnr2.70264","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,9,6]],"date-time":"2025-09-06T03:00:58Z","timestamp":1757127658000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cnr2.70264"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,8]]},"references-count":31,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2025,8]]}},"alternative-id":["10.1002\/cnr2.70264"],"URL":"https:\/\/doi.org\/10.1002\/cnr2.70264","archive":["Portico"],"relation":{},"ISSN":["2573-8348","2573-8348"],"issn-type":[{"value":"2573-8348","type":"print"},{"value":"2573-8348","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,8]]},"assertion":[{"value":"2024-06-10","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-06-06","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-08-03","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70264"}}