{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,25]],"date-time":"2025-11-25T07:38:52Z","timestamp":1764056332530,"version":"3.45.0"},"reference-count":31,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2025,11,6]],"date-time":"2025-11-06T00:00:00Z","timestamp":1762387200000},"content-version":"vor","delay-in-days":5,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82200163"],"award-info":[{"award-number":["82200163"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Reports"],"published-print":{"date-parts":[[2025,11]]},"abstract":"ABSTRACT<\/jats:title>\n \n Background<\/jats:title>\n The 7\u2010Dehydrocholesterol reductase (DHCR7), a critical enzyme catalyzing the final step of the cholesterol biosynthesis pathway, has gained attention for its potential role in tumorigenesis. This study systematically investigated the association between DHCR7 expression and oncogenic processes across multiple cancer types.<\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n Multi\u2010omics data were obtained from The Cancer Genome Atlas (\n TCGA<\/jats:styled-content>\n ) and Gene Expression Omnibus (\n GEO<\/jats:styled-content>\n ) repositories.\n DHCR7<\/jats:styled-content>\n expression patterns were analyzed using Oncomine,\n TIMER<\/jats:styled-content>\n , and\n GEPIA<\/jats:styled-content>\n platforms. Prognostic significance was assessed via Kaplan\u2013Meier plotter and\n GEPIA<\/jats:styled-content>\n . Tumor stage correlations and immune\/molecular subtype associations were evaluated using\n TISIDB<\/jats:styled-content>\n .\n SangerBox<\/jats:styled-content>\n facilitated analysis of DHCR7's associations with immune checkpoint (\n ICP<\/jats:styled-content>\n ) molecules, tumor mutational burden (\n TMB<\/jats:styled-content>\n ), microsatellite instability (\n MSI<\/jats:styled-content>\n ), mutant\u2010allele tumor heterogeneity (\n MATH<\/jats:styled-content>\n ), neoantigen load, and immune cell infiltration.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n DHCR7<\/jats:styled-content>\n exhibited significant overexpression in most malignancies, correlating with advanced tumor stage (\n p<\/jats:italic>\n \u2009<\u20090.05), metastatic progression, and reduced overall survival (\n HR<\/jats:styled-content>\n \u2009=\u20091.34, 95%\n CI<\/jats:styled-content>\n : 1.18\u20131.52). Strong associations emerged between\n DHCR7<\/jats:styled-content>\n expression and critical immunomodulatory parameters: positive correlations with\n ICPs<\/jats:styled-content>\n (\n PD<\/jats:styled-content>\n \u2010\n L1<\/jats:styled-content>\n :\n r<\/jats:italic>\n \u2009=\u20090.62,\n CTLA4<\/jats:styled-content>\n :\n r<\/jats:italic>\n \u2009=\u20090.58). Significant links to TMB (\n p<\/jats:italic>\n \u2009=\u20092.1e\u22125), MSI (\n p<\/jats:italic>\n \u2009=\u20094.3e\u22124), and MATH (\n p<\/jats:italic>\n \u2009=\u20097.8e\u22123). Distinct immune infiltration patterns, particularly in bladder carcinoma (BLCA), renal clear cell carcinoma (KIRC), and prostate adenocarcinoma (PRAD). Co\u2010expression network analysis revealed DHCR7's involvement in immune response regulation (GO:0002764, FDR\u2009=\u20090.003), leukocyte differentiation (GO:0002521, FDR\u2009=\u20090.012), and angiogenesis (GO:0001525, FDR\u2009=\u20090.018).\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions<\/jats:title>\n \n These pan\u2010cancer analyses identify\n DHCR7<\/jats:styled-content>\n as a multifaceted biomarker with dual prognostic and immunotherapeutic relevance. Its involvement in tumor immune microenvironment modulation suggests potential as a therapeutic target.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1002\/cnr2.70376","type":"journal-article","created":{"date-parts":[[2025,11,7]],"date-time":"2025-11-07T01:29:23Z","timestamp":1762478963000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["DHCR7<\/scp>\n as a Prognostic and Immunological Biomarker in Human Pan\u2010Cancer: A Comprehensive Evaluation"],"prefix":"10.1002","volume":"8","author":[{"given":"Xianghua","family":"Wu","sequence":"first","affiliation":[{"name":"Department of Neurology, the First People's Hospital of Yuhang District Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8657-4210","authenticated-orcid":false,"given":"Weiwei","family":"Zheng","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, the First Affiliated Hospital of USTC, Division of Life Science and Medicine University of Science and Technology of China Hefei China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Neurology, the First People's Hospital of Yuhang District Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dan","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Neurology, the First People's Hospital of Yuhang District Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6640-5428","authenticated-orcid":false,"given":"Zhaoxing","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Hematology (Key Laboratory of Cancer Prevention and Intervention China National Ministry of Education), the Second Affiliated Hospital, College of Medicine Hangzhou China"},{"name":"Cancer Institute, Zhejiang 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China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,11,6]]},"reference":[{"key":"e_1_2_13_2_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M101661200"},{"key":"e_1_2_13_3_1","doi-asserted-by":"publisher","DOI":"10.1136\/jmg.39.6.e31"},{"key":"e_1_2_13_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gim.2025.101450"},{"key":"e_1_2_13_5_1","doi-asserted-by":"publisher","DOI":"10.1155\/2022\/8383885"},{"key":"e_1_2_13_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13046-023-02611-6"},{"key":"e_1_2_13_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellsig.2022.110553"},{"key":"e_1_2_13_8_1","doi-asserted-by":"publisher","DOI":"10.2174\/0115680096363566250119155918"},{"key":"e_1_2_13_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-023-06983-9"},{"key":"e_1_2_13_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-023-06878-9"},{"key":"e_1_2_13_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2019.11.015"},{"key":"e_1_2_13_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2025.04.024"},{"key":"e_1_2_13_13_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsptsci.2c00051"},{"key":"e_1_2_13_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btz210"},{"key":"e_1_2_13_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neo.2022.01.001"},{"key":"e_1_2_13_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkx1090"},{"key":"e_1_2_13_17_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22052622"},{"key":"e_1_2_13_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41422-020-0343-4"},{"key":"e_1_2_13_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41392-020-00449-4"},{"key":"e_1_2_13_20_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12935-019-1091-8"},{"key":"e_1_2_13_21_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2021.672356"},{"key":"e_1_2_13_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccell.2020.10.001"},{"key":"e_1_2_13_23_1","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2020.571545"},{"key":"e_1_2_13_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.plipres.2016.09.003"},{"key":"e_1_2_13_25_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-23-3703"},{"key":"e_1_2_13_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2024.216609"},{"issue":"7","key":"e_1_2_13_27_1","first-page":"5967","article-title":"Identification 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