{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,26]],"date-time":"2026-03-26T05:21:59Z","timestamp":1774502519179,"version":"3.50.1"},"reference-count":58,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T00:00:00Z","timestamp":1773792000000},"content-version":"vor","delay-in-days":17,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82003301"],"award-info":[{"award-number":["82003301"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Reports"],"published-print":{"date-parts":[[2026,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Microsatellites stability (MSS) colon cancer patients exhibit a significant suppressive immune status, and the functional status of tumor NLRP3 immunosomes plays an important role in regulating the tumor immune microenvironment, but whether they are involved in the regulation of immunosuppression in MSS patients is unclear. Therefore, further exploration of the relevant molecular mechanisms is urgently needed.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>The Cancer Genome Atlas\u2010Colorectal Cancer (TCGA\u2010COAD) Masked Somatic Mutation data, clinicopathological data were obtained, analyzed, and visualized using the \u2018maftools\u2019 in R package. Tissue microarray (TMA) used for this study includes 100 unselected, non\u2010consecutive, primary, and sporadic CRCs treated between April 2006 and October 2010 in Tianjin Medical University General Hospital and 60 adjacent noncancerous tissues. Demographic and clinicopathological variables were collected, and the clinical value and prognostic impact of NLRP3 expression were analyzed. Tissue immunofluorescence (IF) was applied to investigate the colocalization expression of NLRP3 and ASC in tumor cells. The Vectra 3.0 Automated Quantitative Pathology Imaging System was used to obtain spectral information the NLRP3\u2010ASC colocalization was analyzed by the Fiji Plugin \u201cColoc2\u201d. Cytotoxic T lymphocytes and M2 macrophages in tumor tissue were evaluated by immunohistochemistry.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results and Conclusion<\/jats:title>\n                    <jats:p>In patients with MSS\u2010CRC, aberrant activation of NLRP3 immunosome was significantly associated with lymph node metastasis of tumors. It is also closely related to the polarization of M2 macrophages in the tumor microenvironment, and further affects the infiltration of CD8+T lymphocytes, thereby creating a suppressive immune microenvironment.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/cnr2.70470","type":"journal-article","created":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T09:01:20Z","timestamp":1773824480000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["The Aberrant Activation of\n                    <scp>NLRP3<\/scp>\n                    in Microsatellites Stability Colon Cancer Promotes\n                    <scp>M2<\/scp>\n                    Macrophage Polarization Based on the\n                    <scp>TCGA<\/scp>\n                    Database and Tissue Microarray Analysis"],"prefix":"10.1002","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0915-1588","authenticated-orcid":false,"given":"Li","family":"Lu","sequence":"first","affiliation":[{"name":"Department of General Surgery Tianjin Medical University General Hospital  Tianjin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Menglin","family":"Wu","sequence":"additional","affiliation":[{"name":"Radiology Department Second Hospital of Tianjin Medical University  Tianjin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xunzhen","family":"Jiang","sequence":"additional","affiliation":[{"name":"Department of General Surgery Tianjin Medical University General Hospital  Tianjin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tong","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of General Surgery Tianjin Medical University General Hospital  Tianjin 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