{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,9]],"date-time":"2026-03-09T04:59:29Z","timestamp":1773032369696,"version":"3.50.1"},"reference-count":24,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T00:00:00Z","timestamp":1771459200000},"content-version":"vor","delay-in-days":18,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100021510","name":"Department of Haematology, Christian Medical College, Vellore","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100021510","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Reports"],"published-print":{"date-parts":[[2026,2]]},"abstract":"ABSTRACT<\/jats:title>\n \n Background<\/jats:title>\n \n The pathogenesis of diffuse large B\u2010cell lymphoma (DLBCL) remains unclear. Zinc finger protein 382 (\n ZNF382<\/jats:italic>\n ) expression is significantly downregulated in DLBCL, which is associated with poor prognosis. However, the underlying mechanisms remain unknown.\n <\/jats:p>\n <\/jats:sec>\n \n Aims<\/jats:title>\n \n To investigate the association between DNA methylation of the promoter region of\n ZNF382<\/jats:italic>\n and\n ZNF382 expression<\/jats:italic>\n with the occurrence and progression of DLBCL and to analyze the clinical significance of\n ZNF382<\/jats:italic>\n in DLBCL.\n <\/jats:p>\n <\/jats:sec>\n \n Methods and Results<\/jats:title>\n \n DLBCL cell lines and reactive hyperplastic lymph node tissues were used as the experimental subjects. Methylation\u2010specific polymerase chain reaction and reverse\u2010transcription polymerase chain reaction analyses revealed significantly higher methylation of the promoter region of\n ZNF382<\/jats:italic>\n (\n p<\/jats:italic>\n \u2009<\u20090.0001), whereas\n ZNF382<\/jats:italic>\n mRNA expression was significantly lower (\n p<\/jats:italic>\n \u2009<\u20090.01) in DLBCL cells compared with those in reactive hyperplastic lymph node tissues. Cells were treated with the demethylating agent decitabine (DAC), and the methylation status of the promoter and expression levels of\n ZNF382<\/jats:italic>\n were determined.\n ZNF382<\/jats:italic>\n promoter methylation was significantly reduced (\n p<\/jats:italic>\n \u2009<\u20090.01) in DLBCL cells, whereas\n ZNF382<\/jats:italic>\n mRNA expression was significantly increased (\n p<\/jats:italic>\n \u2009<\u20090.01) compared with those in the control cells. Furthermore, compared with that in the blank control group, the apoptosis rate of DLBCL cells was significantly increased following DAC intervention (\n p<\/jats:italic>\n \u2009<\u20090.01). A cell model of\n ZNF382<\/jats:italic>\n overexpression was constructed, and its proliferation, migration, and clonogenic capacities were detected using CCK\u20108, Transwell, and soft agar assays, respectively. Compared with those of the vector control group, the cell proliferation, migration, and clone formation abilities of the\n ZNF382<\/jats:italic>\n overexpression group were significantly inhibited (\n p<\/jats:italic>\n \u2009<\u20090.01).\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n \n DNA hypermethylation in the promoter region of\n ZNF382<\/jats:italic>\n and low\n ZNF382<\/jats:italic>\n mRNA expression are closely related to the occurrence and progression of DLBCL. Moreover,\n ZNF382<\/jats:italic>\n overexpression significantly increased apoptosis and inhibited cell proliferation, migration, and clone formation. Therefore,\n ZNF382<\/jats:italic>\n has potential as a therapeutic target in the treatment of DLBCL.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1002\/cnr2.70502","type":"journal-article","created":{"date-parts":[[2026,2,20]],"date-time":"2026-02-20T03:25:31Z","timestamp":1771557931000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["DNA<\/scp>\n Hypermethylation of the\n \n ZNF382<\/i>\n <\/scp>\n Promoter Region and Low\n mRNA<\/scp>\n Expression of\n \n ZNF382<\/i>\n <\/scp>\n Promote Diffuse Large B\u2010Cell Lymphoma Occurrence and Progression"],"prefix":"10.1002","volume":"9","author":[{"given":"Wanhua","family":"An","sequence":"first","affiliation":[{"name":"Department of Hematology Luoyang Central Hospital Affiliated to Zhengzhou University Luoyang China"},{"name":"Lincoln University College Petaling Jaya Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2505-8877","authenticated-orcid":false,"given":"Shuli","family":"Guo","sequence":"additional","affiliation":[{"name":"Department of Hematology Luoyang Central Hospital Affiliated to Zhengzhou University Luoyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sizhe","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Hematology Luoyang Central Hospital Affiliated to Zhengzhou University Luoyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pengli","family":"Xiao","sequence":"additional","affiliation":[{"name":"Department of Hematology Luoyang Central Hospital Affiliated to Zhengzhou University Luoyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emma","family":"Mi","sequence":"additional","affiliation":[{"name":"Nuffield Department of Primary Care Health Sciences Radcliffe Observatory Quarter, University of Oxford Oxford UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3577-5861","authenticated-orcid":false,"given":"Huirui","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Hematology Luoyang Central Hospital Affiliated to Zhengzhou University Luoyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,2,19]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21357"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood\u20102017\u201003\u2010769620"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1182\/bloodadvances.2020001696"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.7683\/xxyxyxb.2024.01.007"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrg2538"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12885\u2010023\u201010779\u20105"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cca.2019.10.021"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-018-0604-z"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41388\u2010019\u20100759\u20109"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.3892\/ijo.2018.4446"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41571\u2010018\u20100004\u20104"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature05915"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M203009200"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/12659"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092\u20108674(86)90263\u20101"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood\u20102010\u201005\u2010285320"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40168\u2010020\u201000847\u20104"},{"key":"e_1_2_12_19_1","doi-asserted-by":"publisher","DOI":"10.1097\/MD.0000000000006097"},{"key":"e_1_2_12_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2018.08.099"},{"key":"e_1_2_12_21_1","doi-asserted-by":"publisher","DOI":"10.1080\/10428194.2019.1697812"},{"key":"e_1_2_12_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjh.16686"},{"key":"e_1_2_12_23_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.2021011679"},{"key":"e_1_2_12_24_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.18.02151"},{"key":"e_1_2_12_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10495\u2010023\u201001918\u20101"}],"container-title":["Cancer 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