{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T19:09:56Z","timestamp":1772996996925,"version":"3.50.1"},"reference-count":45,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2026,2,22]],"date-time":"2026-02-22T00:00:00Z","timestamp":1771718400000},"content-version":"vor","delay-in-days":21,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100004775","name":"Natural Science Foundation of Gansu Province","doi-asserted-by":"publisher","award":["25JRRA1001"],"award-info":[{"award-number":["25JRRA1001"]}],"id":[{"id":"10.13039\/501100004775","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Reports"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Colon adenocarcinoma (COAD), although the third\u2010most common type of gastrointestinal tumors, still lacks specific biomarkers for early diagnosis, treatment, and prognosis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Aims<\/jats:title>\n                    <jats:p>This study aimed to evaluate the CD276 in tumorigenesis, prognosis and immunity for colon adenocarcinoma.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods and Results<\/jats:title>\n                    <jats:p>\n                      The CD276 expression in colon adenocarcinoma was established by using RNA\u2010sequencing transcriptomic data of The Cancer Genome Atlas (TCGA) databases. The biological functions of CD276 were evaluated using the Metascape database and Gene Set Enrichment Analysis (GSEA). The association between CD276 and immune cell infiltration was investigated by TIMER website. Correlation analysis was performed between CD276 expression and clinicopathological characteristics. CD276 expression was significantly elevated in colon adenocarcinoma tumor (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.0001). High CD276 was associated with microsatellite instability (MSI) status, patients' survival, and disease progression. Cox regression analysis revealed that CD276 was a risk factor for overall survival [hazard ratio (HR): 1.848,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20092.64E\u221203], disease\u2010specific survival (HR: 2.406,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20095.35E\u221204), and progression\u2010free interval (HR: 1.772,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20092.04E\u221203). Moreover, CD276 level was significantly associated with tumor immune cell infiltration, biomarkers of immune cells, and immune checkpoint expression.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Our analyses indicated that increased CD276 may contribute to colon adenocarcinoma development by activating tumor\u2010promoting signal pathways and altering the immune microenvironment.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/cnr2.70503","type":"journal-article","created":{"date-parts":[[2026,2,23]],"date-time":"2026-02-23T04:25:36Z","timestamp":1771820736000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["The Bioinformatics and Experimental Analysis of\n                    <scp>CD276<\/scp>\n                    for Prognosis and Immune Infiltrates in Colon Adenocarcinoma"],"prefix":"10.1002","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0655-2562","authenticated-orcid":false,"given":"Rui","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Oncology The First Hospital of Lanzhou University  Lanzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6643-4686","authenticated-orcid":false,"given":"Tao","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Oncology The First Hospital of Lanzhou University  Lanzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4569-6358","authenticated-orcid":false,"given":"Jingru","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Oncology The First Hospital of Lanzhou University  Lanzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-1753-8619","authenticated-orcid":false,"given":"Longxia","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Oncology The First Hospital of Lanzhou University  Lanzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5498-6130","authenticated-orcid":false,"given":"Fei","family":"Su","sequence":"additional","affiliation":[{"name":"Department of Oncology The First Hospital of Lanzhou University  Lanzhou China"},{"name":"School of Basic Medical Sciences Lanzhou University  Lanzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,2,22]]},"reference":[{"key":"e_1_2_14_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules27113545"},{"key":"e_1_2_14_3_1","doi-asserted-by":"publisher","DOI":"10.1080\/14712598.2020.1815704"},{"key":"e_1_2_14_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2018.10.014"},{"key":"e_1_2_14_5_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21871"},{"key":"e_1_2_14_6_1","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2020.546110"},{"key":"e_1_2_14_7_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.21114"},{"key":"e_1_2_14_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2021.108153"},{"key":"e_1_2_14_9_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-20-2584"},{"key":"e_1_2_14_10_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2021.701006"},{"key":"e_1_2_14_11_1","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2021.654684"},{"key":"e_1_2_14_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcancer.2020.07.001"},{"key":"e_1_2_14_13_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-014-0550-8"},{"key":"e_1_2_14_14_1","unstructured":"R.Kolde \u201cpheatmap: Pretty Heatmaps \u201d 2019."},{"key":"e_1_2_14_15_1","unstructured":"K.Blighe S.Rana andM.Lewis \u201cEnhanced Volcano: Publication\u2010Ready Volcano Plots With Enhanced Colouring and Labeling \u201d 2022."},{"key":"e_1_2_14_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkx247"},{"key":"e_1_2_14_17_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-17-0307"},{"key":"e_1_2_14_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccell.2017.03.005"},{"key":"e_1_2_14_19_1","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2019.01466"},{"key":"e_1_2_14_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.26238"},{"key":"e_1_2_14_21_1","doi-asserted-by":"publisher","DOI":"10.2147\/OTT.S201121"},{"key":"e_1_2_14_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41375-018-0331-6"},{"key":"e_1_2_14_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10549-022-06520-8"},{"key":"e_1_2_14_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-020-2252-3"},{"key":"e_1_2_14_25_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22094327"},{"key":"e_1_2_14_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/ecc.13432"},{"key":"e_1_2_14_27_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13277-013-0763-6"},{"key":"e_1_2_14_28_1","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2021.763527"},{"key":"e_1_2_14_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.autrev.2022.103041"},{"issue":"7","key":"e_1_2_14_30_1","doi-asserted-by":"crossref","DOI":"10.1136\/jitc-2021-002591","article-title":"Mechanisms of Regulatory T Cell Infiltration in Tumors: Implications for Innovative Immune Precision Therapies","volume":"9","author":"Nishikawa H.","year":"2021","journal-title":"Journal for Immunotherapy of Cancer"},{"key":"e_1_2_14_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tranon.2020.100930"},{"key":"e_1_2_14_32_1","doi-asserted-by":"publisher","DOI":"10.1111\/imr.12978"},{"key":"e_1_2_14_33_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40425-018-0349-3"},{"key":"e_1_2_14_34_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10147-019-01548-1"},{"key":"e_1_2_14_35_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13045-021-01085-3"},{"key":"e_1_2_14_36_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2021.636081"},{"key":"e_1_2_14_37_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells10051152"},{"key":"e_1_2_14_38_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-18-4070"},{"key":"e_1_2_14_39_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2018.36.4_suppl.553"},{"key":"e_1_2_14_40_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa2017699"},{"key":"e_1_2_14_41_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41401-023-01118-2"},{"key":"e_1_2_14_42_1","doi-asserted-by":"publisher","DOI":"10.1136\/jitc-2024-009710"},{"key":"e_1_2_14_43_1","doi-asserted-by":"publisher","DOI":"10.1158\/1535-7163.MCT-21-0554"},{"key":"e_1_2_14_44_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-25-0634"},{"key":"e_1_2_14_45_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.34813"},{"key":"e_1_2_14_46_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00432-023-05248-2"}],"container-title":["Cancer Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cnr2.70503","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/cnr2.70503","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cnr2.70503","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T16:16:28Z","timestamp":1772986588000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cnr2.70503"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,2]]},"references-count":45,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2026,2]]}},"alternative-id":["10.1002\/cnr2.70503"],"URL":"https:\/\/doi.org\/10.1002\/cnr2.70503","archive":["Portico"],"relation":{},"ISSN":["2573-8348","2573-8348"],"issn-type":[{"value":"2573-8348","type":"print"},{"value":"2573-8348","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,2]]},"assertion":[{"value":"2025-05-31","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-02-03","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-02-22","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70503"}}