{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T13:06:13Z","timestamp":1789563973559,"version":"build-2803163510"},"reference-count":40,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,3,30]],"date-time":"2026-03-30T00:00:00Z","timestamp":1774828800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,3,30]],"date-time":"2026-03-30T00:00:00Z","timestamp":1774828800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100004681","name":"Higher Education Commission, Pakistan","doi-asserted-by":"publisher","award":["20\u201011952\/NRPU\/RGM\/R&D\/HEC"],"award-info":[{"award-number":["20\u201011952\/NRPU\/RGM\/R&D\/HEC"]}],"id":[{"id":"10.13039\/501100004681","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Reports"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Triple\u2010negative breast cancer (TNBC) is known for its more aggressive clinical behavior, poor prognosis, and distinctive patterns of metastasis. Neoadjuvant chemotherapy (NAC) can influence both tumor cells and the tumor microenvironment. Emerging evidence highlights the critical role of actin cytoskeletal dynamics in cancer progression.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Aims<\/jats:title>\n                    <jats:p>This study aimed to explore the expression of two actin binding proteins, profilin\u20101 (PFN\u20101) and transgelin\u20102 (TAGLN\u20102), before and after NAC. Their prognostic significance, particularly in response to NAC in TNBC, has not been fully elucidated. Their expression patterns may provide valuable insight into their prognostic value and treatment response.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods and Results<\/jats:title>\n                    <jats:p>\n                      This quasi\u2010experimental study included 54 females (aged 24\u201345\u2009years) diagnosed with TNBC pre\u2010 and post\u2010treated NAC and age matched healthy donors (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u200930). The strategy encompasses sequential high\u2010resolution Reverse\u2010Phase Liquid Chromatography Mass Spectrometry (RPLC\u2010MS\/MS) for identification of peptides followed by ELISA and RT\u2010qPCR. An\n                      <jats:italic>in silico<\/jats:italic>\n                      analysis, STRING and KEGG analysis were also employed. The PFN\u20101 protein and gene expression in early stages (I &amp; II) of pre\u2010NAC were significantly (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.0001) higher. However, in the post\u2010NAC patients with advanced stages, expression was reduced. The TGLN\u20102 protein concentration was significantly higher (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.0001) in the pre\u2010NAC group at advanced stages compared with post\u2010NAC patients. Gene expression of TGLN\u20102 is highly overexpressed in both groups at advanced stages. Moreover, tumor suppressor gene PTEN was downregulated at early stages of pre and post\u2010NAC groups and upregulated at advanced stages of post\u2010NAC. STRING and KEGG analysis showed that PFN\u20101 and TGLN\u20102 both displayed association with regulation of actin cytoskeleton.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Aberrantly expression of actin binding proteins, PFN\u20101 and TAGLN\u20102 and tumor suppressor gene PTEN in pre and post\u2010NAC patients in different tumor stages compared with healthy individuals can provide valuable prognostic information and may have potential as a therapeutic target. The observed interactions of PFN\u20101 with VASP and potential crosstalk with TAGLN\u20102 point toward their collective role in actin cytoskeleton regulation and tumorigenesis.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/cnr2.70529","type":"journal-article","created":{"date-parts":[[2026,3,30]],"date-time":"2026-03-30T23:59:24Z","timestamp":1774915164000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Profillin\u20101 and Transgelin\u20102: Actin Binding Proteins Expression in Early and Advanced Stages of Triple\u2010Negative Breast Cancer Receiving Neoadjuvant Chemotherapy"],"prefix":"10.1002","volume":"9","author":[{"given":"Moazzam Ali","family":"Shahid","sequence":"first","affiliation":[{"name":"Department of Biochemistry Ziauddin University and Hospital  Karachi 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