{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,27]],"date-time":"2026-05-27T15:02:19Z","timestamp":1779894139924,"version":"3.53.1"},"reference-count":51,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T00:00:00Z","timestamp":1777593600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T00:00:00Z","timestamp":1777593600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Reports"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Oral squamous cell carcinoma (OSCC) is marked by frequent recurrence rates and an unclear etiology, underscoring the critical need for early detection to improve therapeutic outcomes and reduce healthcare costs. MicroRNAs (miRNAs) have emerged as key regulators of oral carcinogenesis by modulating gene expression at the posttranscriptional level and influencing various aspects of cellular physiology.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>This study aimed to comprehensively evaluate the prognostic significance of miR\u20101304 and miR\u20104652 expression levels in patients with OSCC, and to explore their potential as predictive biomarkers for disease progression and patient survival.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>TargetScan was used to predict potential gene interactions of the microRNAs. Subsequently, the expression levels of C\u2010Myc and the microRNAs miR\u20101304\u20103p and miR\u20104652\u20105p were evaluated in 30 pairs of OSCC and adjacent normal tissue samples. qRT\u2010PCR analyses were performed to compare the expression of these molecules between tumor and normal tissues. Additionally, receiver operating characteristic (ROC) curves were generated to assess the potential diagnostic value of these microRNAs in OSCC.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Result<\/jats:title>\n                    <jats:p>\n                      The expression levels of miR\u20101304, miR\u20104652, and C\u2010Myc were significantly higher in OSCC tissues compared to their matched adjacent non\u2010tumor tissues (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.0001). Notably, high C\u2010Myc expression was significantly correlated with both tumor grade (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.003) and tumor stage (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.005). ROC curve analysis demonstrated that the areas under the curve (AUCs) for C\u2010Myc, hsa\u2010miR\u20101304, and hsa\u2010miR\u20104652 were 0.99, 0.99, and 0.95, respectively (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.0001), indicating strong diagnostic potential.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>These findings suggest that the upregulation of miR\u20101304 and miR\u20104652 could be used as biomarkers in OSCC. However, more studies with large samples are necessary.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/cnr2.70552","type":"journal-article","created":{"date-parts":[[2026,5,2]],"date-time":"2026-05-02T03:11:33Z","timestamp":1777691493000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Identification of Critical\n                    <scp>miRNAs miR<\/scp>\n                    \u20104652 and\n                    <scp>miR<\/scp>\n                    \u20101304 as Novel Diagnostic Markers for Oral Squamous Cell Carcinoma"],"prefix":"10.1002","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2405-3161","authenticated-orcid":false,"given":"Sara Bagheri","family":"Farahani","sequence":"first","affiliation":[{"name":"Department of Biology ET.C., Islamic Azad University  Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-0972-3790","authenticated-orcid":false,"given":"Ehsan","family":"Keramati","sequence":"additional","affiliation":[{"name":"Department of Biology ET.C., Islamic Azad University  Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-6477-770X","authenticated-orcid":false,"given":"Marziyeh","family":"Etesami","sequence":"additional","affiliation":[{"name":"Basic and Molecular Epidemiology of Gastrointestinal Disorders Research Center, Research Institute for Gastroenterology and Liver Diseases Shahid Beheshti University of Medical Sciences  Tehran Iran"},{"name":"Department of Genetics TeMS.C., Islamic Azad University  Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2772-5859","authenticated-orcid":false,"given":"Faranak","family":"Jamshidian","sequence":"additional","affiliation":[{"name":"Department of Biology ET.C., Islamic Azad University  Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,5,1]]},"reference":[{"issue":"2","key":"e_1_2_14_2_1","first-page":"42","article-title":"Epidemiology, Risk Factors, and Prevention of Head and Neck Squamous Cell Carcinoma","volume":"11","author":"Barsouk A.","year":"2023","journal-title":"Medical Science"},{"key":"e_1_2_14_3_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.70048"},{"key":"e_1_2_14_4_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12903-025-05850-5"},{"key":"e_1_2_14_5_1","volume-title":"Oral and Maxillofacial Pathology\u2010E\u2010Book","author":"Neville B. W.","year":"2023"},{"key":"e_1_2_14_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc.2018.11"},{"key":"e_1_2_14_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10565-025-10048-0"},{"key":"e_1_2_14_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jlb.2025.100285"},{"key":"e_1_2_14_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.genrep.2025.102208"},{"key":"e_1_2_14_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41420-024-02146-5"},{"key":"e_1_2_14_11_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13046-025-03391-x"},{"key":"e_1_2_14_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/odi.14758"},{"key":"e_1_2_14_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gene.2025.149382"},{"key":"e_1_2_14_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/jre.13078"},{"key":"e_1_2_14_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-021-86316-w"},{"key":"e_1_2_14_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41420-022-00948-z"},{"key":"e_1_2_14_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijom.2026.01.004"},{"issue":"8","key":"e_1_2_14_18_1","first-page":"921","article-title":"The Role of Non\u2010Coding RNAs in the Regulation of the Proto\u2010Oncogene MYC in Different Types of Cancer","volume":"9","author":"Stasevich E. M.","year":"2021","journal-title":"Biomedicine"},{"key":"e_1_2_14_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gendis.2024.101435"},{"key":"e_1_2_14_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-022-35305-2"},{"key":"e_1_2_14_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.omton.2024.200782"},{"key":"e_1_2_14_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-020-2699-2"},{"key":"e_1_2_14_23_1","doi-asserted-by":"publisher","DOI":"10.3390\/ncrna11020027"},{"key":"e_1_2_14_24_1","doi-asserted-by":"publisher","DOI":"10.7717\/peerj.18072"},{"key":"e_1_2_14_25_1","first-page":"1","article-title":"Association Between miR\u2010539 and miR\u20106824 and Their Target Gene, MAP2K1, Expression Level in Oral Cancer","volume":"17","author":"Bagheri Farahani S.","year":"2025","journal-title":"Indian Journal of Surgical Oncology"},{"key":"e_1_2_14_26_1","doi-asserted-by":"publisher","DOI":"10.3390\/books978-3-0365-4415-1"},{"key":"e_1_2_14_27_1","doi-asserted-by":"publisher","DOI":"10.3892\/or.2020.7854"},{"key":"e_1_2_14_28_1","doi-asserted-by":"publisher","DOI":"10.1186\/s41065-026-00649-y"},{"key":"e_1_2_14_29_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v26.i6.670"},{"key":"e_1_2_14_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2019.03.213"},{"key":"e_1_2_14_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/aps.2016.92"},{"key":"e_1_2_14_32_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers13071665"},{"key":"e_1_2_14_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.rpor.2017.10.003"},{"key":"e_1_2_14_34_1","doi-asserted-by":"publisher","DOI":"10.3390\/medicina56100535"},{"key":"e_1_2_14_35_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12943-016-0512-8"},{"issue":"6","key":"e_1_2_14_36_1","first-page":"3751","article-title":"circ_0067934 Increases Bladder Cancer Cell Proliferation, Migration and Invasion Through Suppressing miR\u20101304 Expression and Increasing Myc Expression Levels","volume":"19","author":"Liu Q.","year":"2020","journal-title":"Experimental and Therapeutic Medicine"},{"key":"e_1_2_14_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2012.03.003"},{"key":"e_1_2_14_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.amjms.2019.05.014"},{"key":"e_1_2_14_39_1","doi-asserted-by":"publisher","DOI":"10.3390\/ph15070890"},{"issue":"2","key":"e_1_2_14_40_1","first-page":"2243","article-title":"MicroRNA\u20101294 Inhibited Oral Squamous Cell Carcinoma Growth by Targeting c\u2010Myc","volume":"16","author":"Wang Z.","year":"2018","journal-title":"Oncology Letters"},{"key":"e_1_2_14_41_1","doi-asserted-by":"publisher","DOI":"10.1186\/1756-9966-29-151"},{"key":"e_1_2_14_42_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00432-010-0898-4"},{"key":"e_1_2_14_43_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers13174484"},{"key":"e_1_2_14_44_1","doi-asserted-by":"publisher","DOI":"10.1002\/1878-0261.13319"},{"key":"e_1_2_14_45_1","doi-asserted-by":"publisher","DOI":"10.1080\/21691401.2019.1671857"},{"issue":"3","key":"e_1_2_14_46_1","first-page":"965","article-title":"Circulating miRNA as a Biomarker in Oral Cancer Liquid Biopsy","volume":"11","author":"Roi A.","year":"2023","journal-title":"Biomedicine"},{"issue":"6","key":"e_1_2_14_47_1","first-page":"1150","article-title":"Oxidative Stress in the Pathogenesis of Oral Cancer","volume":"12","author":"Ionescu C.","year":"2024","journal-title":"Biomedicine"},{"key":"e_1_2_14_48_1","doi-asserted-by":"publisher","DOI":"10.4103\/0973-1482.151935"},{"key":"e_1_2_14_49_1","doi-asserted-by":"publisher","DOI":"10.1155\/2020\/5867064"},{"key":"e_1_2_14_50_1","doi-asserted-by":"publisher","DOI":"10.1155\/2018\/9303094"},{"key":"e_1_2_14_51_1","doi-asserted-by":"publisher","DOI":"10.1128\/mcb.4.11.2486-2497.1984"},{"key":"e_1_2_14_52_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1026384402585"}],"container-title":["Cancer Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cnr2.70552","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/cnr2.70552","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cnr2.70552","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,27]],"date-time":"2026-05-27T14:03:04Z","timestamp":1779890584000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cnr2.70552"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,5]]},"references-count":51,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2026,5]]}},"alternative-id":["10.1002\/cnr2.70552"],"URL":"https:\/\/doi.org\/10.1002\/cnr2.70552","archive":["Portico"],"relation":{},"ISSN":["2573-8348","2573-8348"],"issn-type":[{"value":"2573-8348","type":"print"},{"value":"2573-8348","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,5]]},"assertion":[{"value":"2025-07-26","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-04-13","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-05-01","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70552"}}