{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T13:04:53Z","timestamp":1789563893304,"version":"build-2803163510"},"reference-count":27,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T00:00:00Z","timestamp":1786665600000},"content-version":"vor","delay-in-days":13,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["23K21475"],"award-info":[{"award-number":["23K21475"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Reports"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Ovarian carcinosarcoma (OCS) is a rare, aggressive tumor composed of both carcinomatous and sarcomatous components, typically associated with poor prognosis. Due to its rarity, the molecular characteristics, immune microenvironment, and optimal treatment of OCS remain unclear. This study aimed to characterize the genomic profiles, Trop\u20102 expression, and immune microenvironment of the carcinomatous and sarcomatous components of OCS separately, and to explore candidate therapeutic targets for this rare and aggressive tumor.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Case<\/jats:title>\n                    <jats:p>\n                      This retrospective, single\u2010institution study analyzed nine cases of ovarian or fallopian tube carcinosarcoma treated between 2013 and 2023. Targeted next\u2010generation sequencing of 160 cancer\u2010related genes was performed in eight cases, and immunohistochemistry for p53, Trop\u20102, CD8, and PD\u2010L1 was performed in nine cases, with the carcinomatous and sarcomatous components evaluated separately whenever they were separable. All tumors contained high\u2010grade serous carcinoma components. Targeted sequencing revealed\n                      <jats:italic>TP53<\/jats:italic>\n                      mutations in all samples. Most genetic alterations were shared by both components, supporting the role of epithelial\u2010mesenchymal transition in OCS pathogenesis. Amplifications in\n                      <jats:italic>CCND1<\/jats:italic>\n                      ,\n                      <jats:italic>CCNE1<\/jats:italic>\n                      ,\n                      <jats:italic>PRKCI<\/jats:italic>\n                      , and\n                      <jats:italic>GNAS<\/jats:italic>\n                      (37.5%), and\n                      <jats:italic>ERBB2<\/jats:italic>\n                      (12.5%) were detected, and whole genome duplication was observed in two cases (25%). One case (12.5%) showed high microsatellite instability and was associated with Lynch syndrome. Immunohistochemistry showed Trop\u20102 positivity in all carcinomatous components, with a trend toward higher expression compared with sarcomatous areas (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.0625). CD8 and PD\u2010L1 levels were low, consistent with a relatively non\u2010inflamed, immune\u2010cold\u2010like microenvironment.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>These findings suggest molecular similarities between OCS and high\u2010grade serous carcinoma and highlight the role of epithelial\u2010mesenchymal transition. The low immune marker expression suggests limited benefit from immune checkpoint inhibitors, except in select Lynch syndrome cases. In contrast, the consistently high Trop\u20102 expression in the carcinomatous component may warrant further investigation of Trop\u20102\u2010targeted antibody\u2013drug conjugates as a hypothesis\u2010generating therapeutic candidate for OCS.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/cnr2.70659","type":"journal-article","created":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T10:24:36Z","timestamp":1786703076000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Genomic, Trop\u20102, and Immune Profiles of Carcinomatous and Sarcomatous Components in Ovarian Carcinosarcoma"],"prefix":"10.1002","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-5716-3291","authenticated-orcid":false,"given":"Tomomi","family":"Sakamaki","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology Keio University School of Medicine  Tokyo 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