{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T09:59:44Z","timestamp":1790848784052,"version":"4.1.0"},"reference-count":21,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Reports"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Despite significant advances in breast cancer therapy, resistance to apoptosis remains a major obstacle to successful treatment. Lifeguard (LFG), an antiapoptotic membrane protein, has been implicated in breast cancer progression; however, its role in endoplasmic reticulum (ER) stress\u2010associated molecular responses and apoptosis\u2010related gene regulation remains incompletely understood.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Aims<\/jats:title>\n                    <jats:p>This study aimed to investigate molecular changes associated with LFG\u2010targeted siRNA treatment and reduced LFG mRNA expression in human breast cancer cells.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods and Results<\/jats:title>\n                    <jats:p>\n                      The triple\u2010negative breast cancer cell line MDA\u2010MB\u2010231 and the non\u2010tumorigenic mammary epithelial cell line MCF10A were transfected with LFG\u2010specific siRNA. LFG mRNA expression was analyzed by quantitative RT\u2010PCR. Furthermore, ER stress\u2010 and apoptosis\u2010related gene expression was examined using an RT\n                      <jats:sup>2<\/jats:sup>\n                      Profiler PCR Array, and intracellular calcium\u2010associated fluorescence was assessed by fluorescence\u2010based calcium imaging. LFG mRNA expression was markedly reduced following LFG\u2010specific siRNA treatment in MDA\u2010MB\u2010231 cells, whereas a more moderate reduction was observed in MCF10A cells. LFG\u2010targeted siRNA treatment was associated with altered intracellular calcium\u2010associated fluorescence and differential expression of several ER stress\u2010 and apoptosis\u2010related genes, including upregulation of CREB3L3, SREBF1, and INHBE, as well as downregulation of EDEM1, HSPA2, and RRM2.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>These findings demonstrate that LFG\u2010targeted siRNA treatment and reduced LFG mRNA expression are associated with changes in intracellular calcium\u2010related responses and ER stress\u2010 and apoptosis\u2010associated gene expression, particularly in MDA\u2010MB\u2010231 cells. Further studies are required to determine the underlying mechanisms and establish the functional role of LFG in these cellular responses.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/cnr2.70687","type":"journal-article","created":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T08:02:46Z","timestamp":1790841766000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Regulation of\n                    <scp>ER<\/scp>\n                    Stress\u2010Associated Gene Expression by Lifeguard in Human Breast Cancer Cells"],"prefix":"10.1002","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-0931-3179","authenticated-orcid":false,"given":"Inga","family":"Nebel","sequence":"first","affiliation":[{"name":"Department of Plastic, Aesthetic, Hand and Reconstructive Surgery Hannover Medical School  Hannover 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