{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T03:42:38Z","timestamp":1792208558432,"version":"4.4.0"},"reference-count":31,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2026,10,11]],"date-time":"2026-10-11T00:00:00Z","timestamp":1791676800000},"content-version":"vor","delay-in-days":10,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Reports"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background and Objectives<\/jats:title>\n                    <jats:p>Metastasis is the primary cause of mortality in colorectal cancer (CRC), a leading global malignancy. Although metabolic reprogramming is a hallmark of cancer, the stage\u2010associated transcriptional features of metabolic pathways during CRC progression remain incompletely understood. This study aimed to characterize transcriptionally inferred metabolic heterogeneity during CRC progression and identify candidate molecular factors associated with metastasis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We performed an integrated transcriptomic analysis using TCGA and GEO datasets and compared metabolic pathway signatures across normal tissues, primary tumors, and metastatic lesions. An exploratory tumor lactate metabolism score (TLMS) was developed using Cox regression and LASSO analysis. Consensus clustering identified metabolic subtypes, and their associations with computationally estimated immune\u2010cell signatures were assessed. The functional consequences of ENO3 loss were preliminarily evaluated using CRISPR\/Cas9 knockout, cell proliferation assays, and an NSG tail\u2010vein experimental lung colonization model.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>We observed a stage\u2010associated reversal\u2010like transcriptional pattern: primary tumors showed broad suppression\u00a0of\u00a0metabolic pathway signatures relative to normal tissues, whereas metastatic lesions showed reactivation of several signatures, including the pentose phosphate pathway. A six\u2010gene lactate metabolism\u2010related signature stratified patients into\u00a0prognostic groups within the TCGA cohort. The high\u2010risk group showed higher ssGSEA\u2010inferred neutrophil abundance and enrichment of a neutrophil extracellular trap (NET)\u2010associated transcriptional signature. ENO3 expression was associated with poor outcome, while ENO3 knockout reduced MC38 cell proliferation and experimental lung colonization in NSG mice.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>CRC progression is accompanied by stage\u2010associated changes in transcriptomically inferred metabolic programs. TLMS and ENO3 represent exploratory candidate biomarkers that require independent clinical validation and further mechanistic investigation, particularly with respect to lactate metabolism and the tumor immune microenvironment.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/cnr2.70723","type":"journal-article","created":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T04:47:47Z","timestamp":1791780467000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Integrated Transcriptomic Analysis Identifies\n                    <scp>ENO3<\/scp>\n                    as a Candidate Lactate Metabolism\u2010Associated Biomarker in Colorectal Cancer Metastasis"],"prefix":"10.1002","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-2155-5144","authenticated-orcid":false,"given":"Jiahao","family":"Qian","sequence":"first","affiliation":[{"name":"Central Research Laboratory, the First Affiliated Hospital of Soochow University  Suzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miaomiao","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Dermatology The First Affiliated Hospital of Soochow University  Suzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiaqi","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology The First Affiliated Hospital of Soochow University  Suzhou Jiangsu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-3117-6044","authenticated-orcid":false,"given":"Meiling","family":"Gao","sequence":"additional","affiliation":[{"name":"Central Research Laboratory, the First Affiliated Hospital of Soochow University  Suzhou 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