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However, the role of TTBK2 in spinal neurons during spinal cord injury (SCI) and subsequent neural repair remains unclear.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      We manipulated TTBK2 expression in spinal neurons using adenovirus\u2010mediated overexpression and knockdown in\u00a0vitro. Transcriptomic profiling (RNA\u2010sequencing) and RT\u2010qPCR were employed to explore the potential regulatory pathways at the molecular level. In\u00a0vivo,\n                      <jats:italic>Ttbk2<\/jats:italic>\n                      <jats:sup>fl\/fl<\/jats:sup>\n                      \u2010Rosa\u2010CreERT2\n                      <jats:sup>+\/\u2212<\/jats:sup>\n                      conditional knockout mice were subjected to a spinal cord hemisection model. Behavioral assays, immunofluorescence, and biotinylated dextran amine (BDA) tracing were conducted to assess neuronal survival, axonal regeneration, and circuit reorganization.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Here, we demonstrated that intact activity of TTBK2 in PC promoted neural survival, axonal regeneration, and neural circuit remodeling. However, loss of TTBK2 impaired PC function and hindered recovery after SCI.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>These findings extend the role of PCs beyond neurodevelopment, demonstrating that the TTBK2\u2013PC functions as an endogenous repair mechanism after SCI. Targeting this pathway may provide novel therapeutic strategies for enhancing neural regeneration.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/cns.70763","type":"journal-article","created":{"date-parts":[[2026,1,24]],"date-time":"2026-01-24T10:35:17Z","timestamp":1769250917000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["TTBK2\u2010Driven Ciliogenesis Is Required for Intrinsic Neuronal Regeneration After Spinal Cord Injury"],"prefix":"10.1002","volume":"32","author":[{"given":"Renfeng","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Orthopedic Surgery Second Hosp Jilin University  Changchun China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7133-1777","authenticated-orcid":false,"given":"Su","family":"Pan","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery Second Hosp Jilin University  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