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Behavioral tests at 72\u2009h revealed motor impairment and DKI showed increased diffusivity in the somatosensory cortex, thalamus, and striatum in MPTP\u2010treated mice. Notably, at 72\u2009h, the hippocampus showed partial recovery in diffusivity, suggesting adaptive changes or partial restoration. Higher diffusivity was observed in the cortex, striatum, and thalamus in MPTP\u2010treated mice. Furthermore,\n                      <jats:sup>1<\/jats:sup>\n                      H\u2010MRS detected a higher Tau\/tCr in the striatum, while in the hippocampus, lower Gln\/tCr and NAA\/tCr and higher Cho\/NAA were observed at 72\u2009h in MPTP\u2010treated mice, indicating persistent neuronal death and membrane deterioration. Immunofluorescence staining at 72\u2009h confirmed these findings, showing a decrease in NeuN+ neurons and an increase in GFAP+ glial cells in the striatum and hippocampus, indicating neurodegeneration and gliosis. Additionally, MPTP caused a loss of dopaminergic neurons in the substantia nigra and striatum, which likely explains the higher diffusivity shown by DKI.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      These findings demonstrate DKI and\n                      <jats:sup>1<\/jats:sup>\n                      H\u2010MRS are sensitive, non\u2010invasive modalities for detecting and monitoring neurodegenerative microstructural and neurochemical changes, enhancing the understanding of PD\u2010related pathology and progression.\n                    <\/jats:p>\n                  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