{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,30]],"date-time":"2026-10-30T07:30:33Z","timestamp":1793345433272,"version":"4.5.0"},"reference-count":46,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T00:00:00Z","timestamp":1780012800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T00:00:00Z","timestamp":1780012800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["30560042"],"award-info":[{"award-number":["30560042"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81160161"],"award-info":[{"award-number":["81160161"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81360198"],"award-info":[{"award-number":["81360198"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82160255"],"award-info":[{"award-number":["82160255"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100009102","name":"Education Department of Jiangxi Province","doi-asserted-by":"publisher","award":["GJJ13198"],"award-info":[{"award-number":["GJJ13198"]}],"id":[{"id":"10.13039\/501100009102","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100009102","name":"Education Department of Jiangxi Province","doi-asserted-by":"publisher","award":["GJJ170021"],"award-info":[{"award-number":["GJJ170021"]}],"id":[{"id":"10.13039\/501100009102","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010857","name":"Jiangxi Provincial Department of Science and Technology","doi-asserted-by":"publisher","award":["20142BBG70062"],"award-info":[{"award-number":["20142BBG70062"]}],"id":[{"id":"10.13039\/501100010857","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010857","name":"Jiangxi Provincial Department of Science and Technology","doi-asserted-by":"publisher","award":["20171BAB215022"],"award-info":[{"award-number":["20171BAB215022"]}],"id":[{"id":"10.13039\/501100010857","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010857","name":"Jiangxi Provincial Department of Science and Technology","doi-asserted-by":"publisher","award":["20192BAB205043"],"award-info":[{"award-number":["20192BAB205043"]}],"id":[{"id":"10.13039\/501100010857","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010857","name":"Jiangxi Provincial Department of Science and Technology","doi-asserted-by":"publisher","award":["20212BAB216026"],"award-info":[{"award-number":["20212BAB216026"]}],"id":[{"id":"10.13039\/501100010857","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["CNS Neurosci Ther"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Aims<\/jats:title>\n                    <jats:p>Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disease; the precise pathogenesis of sporadic ALS (sALS) has not yet been elucidated up to now. Previous studies revealed that the abnormal alterations of some non\u2010motor neurons (non\u2010MN) were a potential pathogenesis of sALS. Therefore, this study aims to search the potential evidences of non\u2010MN in the pathogenesis of ALS via exploring potential relationships between 5\u2010hydroxytryptamine (5\u2010HT) neurons and the development of ALS.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We employed fluorescent immunohistochemistry to investigate the altered distribution patterns of 5\u2010HT and tryptophan hydroxylase 2 in the spinal cord and brainstem of Tg(SOD1*G93A)1Gur (TG) and wild\u2010type (WT) mice. Additionally, we used western blot to analyze the expression levels of 5\u2010hydroxytryptamine receptor 1A (5\u2010HTR1A) and 5\u2010HTR2A.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Our findings revealed that 5\u2010HT synapses were primarily distributed in the funiculus lateralis, anterior horn, posterior horn, central lateral column, and the area around the central canal of cervical, thoracic, and lumbar segments, and raphe nucleus as well as lateral paragigantocellular nucleus, and gradually reduced following age increase in WT mice. However, 5\u2010HT synapses in the spinal cord and 5\u2010HT neurons in the brainstem gradually increased following the progression of disease and presented a significantly negative correlation between the increased distribution of 5\u2010HT synapses and neurons and the reduction of neural cell number (positively correlated with the increase in neural cell death) at the onset and\/or progression stage of TG mice. 5\u2010HTR1A significantly increased, while 5\u2010HTR2A significantly decreased at the onset stage of TG mice.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Our study speculated that the distribution changes of 5\u2010HT synapses in the spinal cord and 5\u2010HT neurons in the brainstem play a potential protective role in the pathogenesis of sALS through a compensatory 5\u2010HT increase.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/cns.70946","type":"journal-article","created":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T12:42:13Z","timestamp":1780058533000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["5\u2010Hydroxytryptamine Distribution Alteration in Both Neuron and Synapse of Tg(\n                    <scp>SOD1<\/scp>\n                    *\n                    <scp>G93A<\/scp>\n                    )1gur Mice: A Potential Intervention Candidate Strategy for Amyotrophic Lateral Sclerosis"],"prefix":"10.1002","volume":"32","author":[{"given":"Lijun","family":"Zhou","sequence":"first","affiliation":[{"name":"Jiangxi Medical College Nanchang University  Nanchang China"},{"name":"Department of Neurology Jiangxi Provincial People's Hospital, the First Affiliated Hospital of Nanchang Medical College, National Regional Medical Center for Nervous Disease  Nanchang Jiangxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Menghua","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Neurology First Affiliated Hospital of Nanchang University  Nanchang Jiangxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qi","family":"Dai","sequence":"additional","affiliation":[{"name":"Jiangxi Medical College Nanchang University  Nanchang China"},{"name":"Institute of Life Science Nanchang University  Nanchang Jiangxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiwang","family":"Liu","sequence":"additional","affiliation":[{"name":"Jiangxi Medical College Nanchang University  Nanchang China"},{"name":"Basic Medical School Nanchang University  Nanchang Jiangxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cheng","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Neurology Jiangxi Provincial People's Hospital, the First Affiliated Hospital of Nanchang Medical College, National Regional Medical Center for Nervous Disease  Nanchang Jiangxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huifeng","family":"Jiao","sequence":"additional","affiliation":[{"name":"Basic Medical School Nanchang University  Nanchang Jiangxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haili","family":"Pan","sequence":"additional","affiliation":[{"name":"Department of Neurology Jiangxi Provincial People's Hospital, the First Affiliated Hospital of Nanchang Medical College, National Regional Medical Center for Nervous Disease  Nanchang Jiangxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0313-3434","authenticated-orcid":false,"given":"Renshi","family":"Xu","sequence":"additional","affiliation":[{"name":"Jiangxi Medical College Nanchang University  Nanchang China"},{"name":"Department of Neurology Jiangxi Provincial People's Hospital, the First Affiliated Hospital of Nanchang Medical College, National Regional Medical Center for Nervous Disease  Nanchang Jiangxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,5,29]]},"reference":[{"key":"e_1_2_14_2_1","doi-asserted-by":"publisher","DOI":"10.1159\/000351153"},{"key":"e_1_2_14_3_1","doi-asserted-by":"publisher","DOI":"10.15585\/mmwr.ss6508a1"},{"key":"e_1_2_14_4_1","doi-asserted-by":"publisher","DOI":"10.1097\/WCO.0b013e328356d328"},{"key":"e_1_2_14_5_1","doi-asserted-by":"publisher","DOI":"10.4081\/ni.2015.5885"},{"key":"e_1_2_14_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(10)61156-7"},{"key":"e_1_2_14_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mayocp.2018.04.007"},{"key":"e_1_2_14_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrneurol.2011.152"},{"key":"e_1_2_14_9_1","doi-asserted-by":"publisher","DOI":"10.7150\/ijbs.19150"},{"key":"e_1_2_14_10_1","doi-asserted-by":"publisher","DOI":"10.7150\/ijbs.16158"},{"key":"e_1_2_14_11_1","doi-asserted-by":"publisher","DOI":"10.7150\/ijbs.15938"},{"key":"e_1_2_14_12_1","doi-asserted-by":"publisher","DOI":"10.7150\/ijbs.26829"},{"key":"e_1_2_14_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12035-017-0406-9"},{"key":"e_1_2_14_14_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnagi.2017.00351"},{"issue":"4","key":"e_1_2_14_15_1","first-page":"99","article-title":"The Emerging Picture of ALS: A Multisystem, Not Only a Motor Neuron Disease","volume":"155","author":"Silani V.","year":"2017","journal-title":"Archives Italiennes de Biologie"},{"issue":"4","key":"e_1_2_14_16_1","first-page":"118","article-title":"The Multisystem Degeneration Amyotrophic Lateral Sclerosis \u2010 Neuropathological Staging and Clinical Translation","volume":"155","author":"Verde F.","year":"2017","journal-title":"Archives Italiennes de Biologie"},{"key":"e_1_2_14_17_1","doi-asserted-by":"publisher","DOI":"10.1097\/WNR.0b013e328363148c"},{"key":"e_1_2_14_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neulet.2013.11.022"},{"key":"e_1_2_14_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/ana.23937"},{"key":"e_1_2_14_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02260888"},{"key":"e_1_2_14_21_1","doi-asserted-by":"publisher","DOI":"10.1080\/14660820310009389"},{"key":"e_1_2_14_22_1","doi-asserted-by":"publisher","DOI":"10.1093\/brain\/aws274"},{"key":"e_1_2_14_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00401-016-1534-4"},{"key":"e_1_2_14_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/ana.25030"},{"key":"e_1_2_14_25_1","doi-asserted-by":"publisher","DOI":"10.1139\/jpn.0738"},{"key":"e_1_2_14_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2015.02.047"},{"key":"e_1_2_14_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-0173(02)00182-0"},{"key":"e_1_2_14_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchemneu.2003.10.002"},{"key":"e_1_2_14_29_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.8209258"},{"key":"e_1_2_14_30_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0015445"},{"key":"e_1_2_14_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/362059a0"},{"key":"e_1_2_14_32_1","doi-asserted-by":"publisher","DOI":"10.1159\/000369466"},{"key":"e_1_2_14_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbr.2010.04.042"},{"key":"e_1_2_14_34_1","doi-asserted-by":"publisher","DOI":"10.1080\/17482960701856300"},{"key":"e_1_2_14_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijdevneu.2008.06.002"},{"key":"e_1_2_14_36_1","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.22263"},{"key":"e_1_2_14_37_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2001.012668"},{"key":"e_1_2_14_38_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2004.064717"},{"key":"e_1_2_14_39_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1990.tb16870.x"},{"key":"e_1_2_14_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1569-7339(10)70076-X"},{"key":"e_1_2_14_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-2952(03)00556-2"},{"key":"e_1_2_14_42_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1078197"},{"key":"e_1_2_14_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpsychires.2005.05.004"},{"key":"e_1_2_14_44_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0180136"},{"key":"e_1_2_14_45_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0013346"},{"issue":"8","key":"e_1_2_14_46_1","first-page":"528","article-title":"Possible Etiology and Treatment of Amyotrophic Lateral Sclerosis","volume":"38","author":"Holecek V.","year":"2018","journal-title":"Neuro Endocrinology Letters"},{"key":"e_1_2_14_47_1","doi-asserted-by":"publisher","DOI":"10.1152\/jn.00425.2013"}],"container-title":["CNS Neuroscience &amp; Therapeutics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cns.70946","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/cns.70946","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cns.70946","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,30]],"date-time":"2026-10-30T06:31:52Z","timestamp":1793341912000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cns.70946"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,5,29]]},"references-count":46,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2026,6]]}},"alternative-id":["10.1002\/cns.70946"],"URL":"https:\/\/doi.org\/10.1002\/cns.70946","archive":["Portico"],"relation":{},"ISSN":["1755-5930","1755-5949"],"issn-type":[{"value":"1755-5930","type":"print"},{"value":"1755-5949","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,5,29]]},"assertion":[{"value":"2025-09-07","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-05-15","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-05-29","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70946"}}