{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T14:17:05Z","timestamp":1784125025394,"version":"3.55.0"},"reference-count":46,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2026,6,5]],"date-time":"2026-06-05T00:00:00Z","timestamp":1780617600000},"content-version":"vor","delay-in-days":4,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100012226","name":"Fundamental Research Funds for the Central Universities","doi-asserted-by":"publisher","award":["YD9100002033"],"award-info":[{"award-number":["YD9100002033"]}],"id":[{"id":"10.13039\/501100012226","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003995","name":"Natural Science Foundation of Anhui Province","doi-asserted-by":"publisher","award":["2308085QH265"],"award-info":[{"award-number":["2308085QH265"]}],"id":[{"id":"10.13039\/501100003995","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["CNS Neurosci Ther"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>To investigate gait characteristics and plasma biomarkers in individuals with mild cognitive impairment (MCI) stratified by amyloid\u2010\u03b2 (A\u03b2) positivity on positron emission tomography (PET), and to evaluate the predictive value for Alzheimer's disease (AD)\u2013related MCI.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>A total of 168 participants were enrolled, including 50 amyloid PET\u2010negative MCI (MCI\u2010), 51 amyloid PET\u2010positive MCI (MCI+), and 61 cognitively normal (CN) individuals. Gait assessments were conducted using a multi\u2010sensor motion analysis system during dual\u2010task paradigms (cognitive load superimposed on locomotion). Plasma samples were analyzed for neurofilament light chain (NfL), glial fibrillary acidic protein (GFAP), and phosphorylated tau at threonine 217 (p\u2010tau217) via ultra\u2010sensitive immunoassays.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Gait analyses identified 125 features, with 69 distinguishing MCI+ from CN and 36 differentiating MCI+ from MCI\u2010. Receiver operating characteristic (ROC) analyses showed that dual\u2010task gait under the countdown and animal\u2010naming conditions (gait\u2010countdown [GCD] and gait\u2010animal naming [GAN]) discriminated MCI+ from CN with an AUC of 0.850. The combined models integrating GCD and GAN with plasma GFAP or p\u2010tau217 yielded AUCs of 0.919 and 0.951, respectively. Similarly, GCD&amp;GAN features demonstrated an AUC of 0.862 for distinguishing MCI+ from MCI\u2212, with GFAP (AUC\u2009=\u20090.933) and p\u2010tau217 (AUC\u2009=\u20090.986) enhancing predictive performance.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>This study provides evidence that dual\u2010task gait assessments, when combined with plasma biomarkers such as GFAP and p\u2010tau217, may improve the discriminatory power for identifying AD\u2010related MCI. The integration of biomechanical and molecular markers holds promise for advancing early detection strategies and therapeutic monitoring in AD.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/cns.70949","type":"journal-article","created":{"date-parts":[[2026,6,5]],"date-time":"2026-06-05T14:32:02Z","timestamp":1780669922000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Multimodal Integration of Gait Dysfunction, Amyloid\n                    <scp>PET<\/scp>\n                    , and Plasma Biomarkers for Differentiating Etiological Subtypes in Mild Cognitive Impairment"],"prefix":"10.1002","volume":"32","author":[{"given":"Jiaonan","family":"Wu","sequence":"first","affiliation":[{"name":"Department of Neurology, the First Affiliated Hospital of USTC, Division of Life Sciences and Medicine University of Science and Technology of China  Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fang","family":"Tang","sequence":"additional","affiliation":[{"name":"Department of Neurology, the First Affiliated Hospital of USTC, Division of Life Sciences and Medicine University of Science and Technology of China  Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinyi","family":"Lv","sequence":"additional","affiliation":[{"name":"Department of Neurology, the First Affiliated Hospital of USTC, Division of Life Sciences and Medicine University of Science and Technology of China  Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yiwei","family":"Wang","sequence":"additional","affiliation":[{"name":"Graduate School Bengbu Medical University  Bengbu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feng","family":"Gao","sequence":"additional","affiliation":[{"name":"Department of Neurology, the First Affiliated Hospital of USTC, Division of Life Sciences and Medicine University of Science and Technology of China  Hefei China"},{"name":"Neurodegenerative Disorder Research Center, Division of Life Sciences and Medicine University of Science and Technology of China  Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong","family":"Shen","sequence":"additional","affiliation":[{"name":"Department of Neurology, the First Affiliated Hospital of USTC, Division of Life Sciences and Medicine University of Science and Technology of China  Hefei China"},{"name":"Neurodegenerative Disorder Research Center, Division of Life Sciences and Medicine University of Science and Technology of China  Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhaozhao","family":"Cheng","sequence":"additional","affiliation":[{"name":"Department of Neurology, the First Affiliated Hospital of USTC, Division of Life Sciences and Medicine University of Science and Technology of China  Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7694-1366","authenticated-orcid":false,"given":"Jiong","family":"Shi","sequence":"additional","affiliation":[{"name":"Department of Neurology, the First Affiliated Hospital of USTC, Division of Life Sciences and Medicine University of Science and Technology of China  Hefei China"},{"name":"Neurodegenerative Disorder Research Center, Division of Life Sciences and Medicine University of Science and Technology of China  Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,5]]},"reference":[{"issue":"3","key":"e_1_2_14_2_1","first-page":"93","article-title":"Mild Cognitive Impairment: Transition Between Aging and Alzheimer's Disease","volume":"15","author":"Petersen R. 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