{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T04:59:57Z","timestamp":1785301197217,"version":"3.55.0"},"reference-count":46,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T00:00:00Z","timestamp":1784851200000},"content-version":"vor","delay-in-days":23,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82471294"],"award-info":[{"award-number":["82471294"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32271061"],"award-info":[{"award-number":["32271061"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32471062"],"award-info":[{"award-number":["32471062"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["CNS Neurosci Ther"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Aims<\/jats:title>\n                    <jats:p>Midazolam is a sedative that acts on \u03b3\u2010aminobutyric acid type A receptors, but whether its effects are region\u2010specific, especially in the neocortex, and the mechanisms underlying these effects remain unclear.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Using microendoscopic Ca\n                      <jats:sup>2+<\/jats:sup>\n                      imaging and whole\u2010cell recording in brain slices, we systematically investigated the effects of midazolam on excitatory pyramidal neurons and inhibitory parvalbumin\u2010positive interneurons (PV\n                      <jats:sup>+<\/jats:sup>\n                      ) in the mouse auditory cortex (AC) and anterior cingulate cortex (ACC).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Midazolam dose\u2010dependently suppressed pyramidal neurons more potently in the AC than in the ACC (Evoked: pyramidal neurons: 0.65\u2009\u00b1\u20090.30 vs. 1.36\u2009\u00b1\u20090.34,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.0001; Spontaneous: pyramidal neurons: 1.49\u2009\u00b1\u20090.48 vs. 2.25\u2009\u00b1\u20090.42,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.0006). In slices, midazolam reduced the action potential firing of pyramidal neurons in a concentration\u2010dependent manner, with greater sensitivity in the AC. In contrast, PV\n                      <jats:sup>+<\/jats:sup>\n                      neurons showed little change in intrinsic excitability in either region. Functional assays suggested that midazolam responsiveness was associated with GABRA1 expression, which was higher in AC pyramidal neurons than in those of the ACC (1.77\u2009\u00b1\u20090.55 vs. 1.01\u2009\u00b1\u20090.36,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.002), while PV\n                      <jats:sup>+<\/jats:sup>\n                      neurons showed no significant regional difference in GABRA1 expression.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Midazolam suppresses cortical excitatory neurons in a region\u2010 and cell\u2010type\u2010dependent manner that is associated with differential expression of GABRA1.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/cns.71049","type":"journal-article","created":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T12:45:14Z","timestamp":1784897114000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Midazolam Exerts Sedative Effects by Differentially Modulating Cortical Pyramidal Neurons and\n                    <scp>PV<\/scp>\n                    Interneurons"],"prefix":"10.1002","volume":"32","author":[{"given":"Yao","family":"Sun","sequence":"first","affiliation":[{"name":"Department of Anaesthesiology Zhujiang Hospital, Southern Medical University  Guangzhou China"},{"name":"Institute of Perioperative Medicine and Organ Protection, Zhujiang Hospital, Southern Medical University  Guangzhou China"},{"name":"Key Laboratory of Mental Health of the Ministry of Education, Guangdong\u2010Hong Kong\u2010Macao Greater Bay Area Center for Brain Science and Brain\u2010Inspired Intelligence, Guangdong\u2010Hong Kong Joint Laboratory for Psychiatric Disorders, Guangdong Province Key Laboratory of Psychiatric Disorders, Guangdong Basic Research Center of Excellence for Integrated Traditional and Western Medicine for Qingzhi Diseases, School of Biomedical Engineering, Southern Medical University  Guangzhou China"},{"name":"Guangdong Provincial Key Laboratory of Shock and Microcirculation Southern Medical University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4808-7451","authenticated-orcid":false,"given":"Dijia","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Anaesthesiology Zhujiang Hospital, Southern Medical University  Guangzhou China"},{"name":"Institute of Perioperative Medicine and Organ Protection, Zhujiang Hospital, Southern Medical University  Guangzhou China"},{"name":"Key Laboratory of Mental Health of the Ministry of Education, Guangdong\u2010Hong Kong\u2010Macao Greater Bay Area Center for Brain Science and Brain\u2010Inspired Intelligence, Guangdong\u2010Hong Kong Joint Laboratory for Psychiatric Disorders, Guangdong Province Key Laboratory of Psychiatric Disorders, Guangdong Basic Research Center of Excellence for Integrated Traditional and Western Medicine for Qingzhi Diseases, School of Biomedical Engineering, Southern Medical University  Guangzhou China"},{"name":"Guangdong Provincial Key Laboratory of Shock and Microcirculation Southern Medical University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3386-3630","authenticated-orcid":false,"given":"Kaibin","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Anaesthesiology Zhujiang Hospital, Southern Medical University  Guangzhou China"},{"name":"Institute of Perioperative Medicine and Organ Protection, Zhujiang Hospital, Southern Medical University  Guangzhou China"},{"name":"Key Laboratory of Mental Health of the Ministry of Education, Guangdong\u2010Hong Kong\u2010Macao Greater Bay Area Center for Brain Science and Brain\u2010Inspired Intelligence, Guangdong\u2010Hong Kong Joint Laboratory for Psychiatric Disorders, Guangdong Province Key Laboratory of Psychiatric Disorders, Guangdong Basic Research Center of Excellence for Integrated Traditional and Western Medicine for Qingzhi Diseases, School of Biomedical Engineering, Southern Medical University  Guangzhou China"},{"name":"Guangdong Provincial Key Laboratory of Shock and Microcirculation Southern Medical University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mengyu","family":"Yin","sequence":"additional","affiliation":[{"name":"Department of Anaesthesiology Zhujiang Hospital, Southern Medical University  Guangzhou China"},{"name":"Institute of Perioperative Medicine and Organ Protection, Zhujiang Hospital, Southern Medical University  Guangzhou China"},{"name":"Key Laboratory of Mental Health of the Ministry of Education, Guangdong\u2010Hong Kong\u2010Macao Greater Bay Area Center for Brain Science and Brain\u2010Inspired Intelligence, Guangdong\u2010Hong Kong Joint Laboratory for Psychiatric Disorders, Guangdong Province Key Laboratory of Psychiatric Disorders, Guangdong Basic Research Center of Excellence for Integrated Traditional and Western Medicine for Qingzhi Diseases, School of Biomedical Engineering, Southern Medical University  Guangzhou China"},{"name":"Guangdong Provincial Key Laboratory of Shock and Microcirculation Southern Medical University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peiwen","family":"Tang","sequence":"additional","affiliation":[{"name":"Department of Anaesthesiology Zhujiang Hospital, Southern Medical University  Guangzhou China"},{"name":"Institute of Perioperative Medicine and Organ Protection, Zhujiang Hospital, Southern Medical University  Guangzhou China"},{"name":"Key Laboratory of Mental Health of the Ministry of Education, Guangdong\u2010Hong Kong\u2010Macao Greater Bay Area Center for Brain Science and Brain\u2010Inspired Intelligence, Guangdong\u2010Hong Kong Joint Laboratory for Psychiatric Disorders, Guangdong Province Key Laboratory of Psychiatric Disorders, Guangdong Basic Research Center of Excellence for Integrated Traditional and Western Medicine for Qingzhi Diseases, School of Biomedical Engineering, Southern Medical University  Guangzhou China"},{"name":"Guangdong Provincial Key Laboratory of Shock and Microcirculation Southern Medical University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3307-1468","authenticated-orcid":false,"given":"Fengxian","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Anaesthesiology Zhujiang Hospital, Southern Medical University  Guangzhou China"},{"name":"Institute of Perioperative Medicine and Organ Protection, Zhujiang Hospital, Southern Medical University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0319-9022","authenticated-orcid":false,"given":"Junlei","family":"Chang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Biomedical Imaging Science and System Institute of Biomedicine and Biotechnology, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences  Shenzhen Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feixue","family":"Liang","sequence":"additional","affiliation":[{"name":"Department of Anaesthesiology Zhujiang Hospital, Southern Medical University  Guangzhou China"},{"name":"Key Laboratory of Mental Health of the Ministry of Education, Guangdong\u2010Hong Kong\u2010Macao Greater Bay Area Center for Brain Science and Brain\u2010Inspired Intelligence, Guangdong\u2010Hong Kong Joint Laboratory for Psychiatric Disorders, Guangdong Province Key Laboratory 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