{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T05:00:08Z","timestamp":1785301208853,"version":"3.55.0"},"reference-count":55,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T00:00:00Z","timestamp":1785196800000},"content-version":"vor","delay-in-days":27,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82472598"],"award-info":[{"award-number":["82472598"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003453","name":"Natural Science Foundation of Guangdong Province","doi-asserted-by":"publisher","award":["2025A1515010379"],"award-info":[{"award-number":["2025A1515010379"]}],"id":[{"id":"10.13039\/501100003453","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["CNS Neurosci Ther"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Cerebral palsy (CP) is predominantly characterized by spasticity. While botulinum neurotoxin type A (BoNT\u2010A) effectively reduces spasticity, its transient efficacy represents a major clinical limitation. Elucidating the mechanisms that limit its duration of action may inform strategies to prolong therapeutic benefit.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Method<\/jats:title>\n                    <jats:p>A spastic CP rat model was established via carotid artery ligation and hypoxia in 7\u2010day\u2010old Wistar rats. On postnatal Day 21, BoNT\u2010A (5\u2009U\/kg) was administered to the gastrocnemius muscle. Behavioral and molecular biological assessments were performed at 4 and 12\u2009weeks post\u2010injection.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Result<\/jats:title>\n                    <jats:p>\n                      At 4\u2009weeks after injection, the motor performance improved, spasticity decreased (\n                      <jats:italic>p\u2009&lt;<\/jats:italic>\n                      \u20090.05), neuromuscular junction density increased, and neurotrophic factors IGF\u20101, GAP 43, and S100 increased (all\n                      <jats:italic>p\u2009&lt;<\/jats:italic>\n                      \u20090.05). However, these functional and molecular changes diminished by 12\u2009weeks. Further proteomic analysis revealed a shift in pathway enrichment, from protein synthesis and vesicular transport at 4\u2009weeks to metabolic regulation at 12\u2009weeks. Among the differentially expressed proteins, Sar1b and Rtn1, whose expression patterns paralleled with nerve sprouting, may be key regulatory factors.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>These findings indicate that BoNT\u2010A facilitates NMJ recovery through temporal regulation of energy metabolism, protein synthesis, and vesicular transport. Rtn1 and Sar1b may represent candidate molecular targets for extending the therapeutic effects of BoNT\u2010A, although further functional validation is required.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/cns.71057","type":"journal-article","created":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T12:18:35Z","timestamp":1785241115000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Temporal Neuromuscular Adaptations and Proteomic Signatures Following Botulinum Neurotoxin A Injection in Spastic Hemiplegia Rats"],"prefix":"10.1002","volume":"32","author":[{"given":"Mengru","family":"Zhong","sequence":"first","affiliation":[{"name":"Department of Rehabilitation, Guangzhou Women and Children's Medical Center Guangzhou Medical University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huijuan","family":"Lin","sequence":"additional","affiliation":[{"name":"Department 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