{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T07:19:53Z","timestamp":1787901593270,"version":"build-2784847793"},"reference-count":40,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T00:00:00Z","timestamp":1787184000000},"content-version":"vor","delay-in-days":19,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82401559"],"award-info":[{"award-number":["82401559"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100018925","name":"Health Commission of Henan Province","doi-asserted-by":"publisher","award":["YQRC2023012"],"award-info":[{"award-number":["YQRC2023012"]}],"id":[{"id":"10.13039\/100018925","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100018925","name":"Health Commission of Henan Province","doi-asserted-by":"publisher","award":["LHGJ20230327"],"award-info":[{"award-number":["LHGJ20230327"]}],"id":[{"id":"10.13039\/100018925","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["CNS Neurosci Ther"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Intracerebral hemorrhage (ICH) remains one of the most devastating subtypes of stroke, characterized by high mortality and limited therapeutic options. Neuroinflammation is recognized as a critical determinant of secondary brain injury following ICH. Minocycline, a broad\u2010spectrum tetracycline antibiotic, has been shown to attenuate hematoma expansion, reduce blood\u2013brain barrier (BBB) disruption, and improve neurological outcomes in preclinical ICH models. However, its clinical translation has been limited by poor aqueous solubility, low bioavailability, and dose\u2010related systemic toxicity.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We designed a brain\u2010targeted nanotherapeutic system based on minocycline\u2010loaded polymeric nanomicelles conjugated with anti\u2010CD147 monoclonal antibodies (MINO@PNM@CD147). The nanomicelles were synthesized using a co\u2010solvent evaporation method, followed by covalent conjugation of CD147 antibodies via amide bond formation. The physicochemical properties, biocompatibility, and targeting efficiency of the formulation were characterized in\u00a0vitro. Therapeutic efficacy was evaluated in a collagenase\u2010induced ICH mouse model using histopathological, biochemical, and behavioral assessments.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>MINO@PNM@CD147 exhibited a uniform spherical morphology with an average hydrodynamic diameter of 13.5\u2009nm and high colloidal stability. In\u00a0vitro studies demonstrated excellent cellular compatibility and effective suppression of pro\u2010inflammatory mediators in lipopolysaccharide (LPS)\u2010activated BV\u20102 cells. In\u00a0vivo fluorescence imaging confirmed targeted accumulation of MINO@PNM@CD147 in perihematomal regions. Treatment markedly reduced neuronal degeneration, microglia and astrocyte activation, leukocyte infiltration, and cell apoptosis, while significantly attenuating the release of inflammatory mediators. Moreover, MINO@PNM@CD147 administration led to substantial improvements in neurological function compared with free minocycline or non\u2010targeted formulations.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Our findings highlight that MINO@PNM@CD147 combines anti\u2010inflammatory efficacy with precise brain\u2010targeted delivery, thereby offering a potent and safe nanotherapeutic strategy for mitigating neuroinflammation and promoting recovery after ICH. This work provides a promising translational platform for nanomedicine\u2010based interventions in ICH.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/cns.71069","type":"journal-article","created":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T09:58:21Z","timestamp":1787219901000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["<scp>CD147<\/scp>\n                    \u2010Conjugated Minocycline Nanomicelles Alleviate Neuroinflammation and Improve Neurological Function Following Intracerebral Hemorrhage"],"prefix":"10.1002","volume":"32","author":[{"given":"Yang","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Cerebrovascular Diseases The Second Affiliated Hospital of Zhengzhou University  Zhengzhou Henan China"},{"name":"Henan International Joint Laboratory of Intracerebral Hemorrhage and Brain Injury Zhengzhou University  Zhengzhou Henan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yun","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Cerebrovascular Diseases The Second Affiliated Hospital of Zhengzhou University  Zhengzhou Henan China"},{"name":"Henan International Joint Laboratory of Intracerebral Hemorrhage and Brain Injury Zhengzhou University  Zhengzhou Henan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuanyuan","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Cerebrovascular Diseases The Second Affiliated Hospital of Zhengzhou University  Zhengzhou Henan China"},{"name":"Henan International Joint Laboratory of Intracerebral Hemorrhage and Brain Injury Zhengzhou University  Zhengzhou Henan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhe","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Cerebrovascular Diseases The Second Affiliated Hospital of Zhengzhou University  Zhengzhou Henan China"},{"name":"Henan International Joint Laboratory of Intracerebral Hemorrhage and Brain Injury Zhengzhou University  Zhengzhou Henan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qingli","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Cerebrovascular Diseases The Second Affiliated Hospital of Zhengzhou University  Zhengzhou Henan China"},{"name":"Henan International Joint Laboratory of Intracerebral Hemorrhage and Brain Injury Zhengzhou University  Zhengzhou Henan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2600-3563","authenticated-orcid":false,"given":"V. Wee","family":"Yong","sequence":"additional","affiliation":[{"name":"Hotchkiss Brain Institute and Department of Clinical Neurosciences University of Calgary  Calgary Alberta Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruixue","family":"Wei","sequence":"additional","affiliation":[{"name":"Department of Cerebrovascular Diseases The Second Affiliated Hospital of Zhengzhou University  Zhengzhou Henan China"},{"name":"Henan International Joint Laboratory of Intracerebral Hemorrhage and Brain Injury Zhengzhou University  Zhengzhou Henan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4427-7285","authenticated-orcid":false,"given":"Mengzhou","family":"Xue","sequence":"additional","affiliation":[{"name":"Department of Cerebrovascular Diseases The Second Affiliated Hospital of Zhengzhou University  Zhengzhou Henan China"},{"name":"Henan International Joint Laboratory of Intracerebral Hemorrhage and Brain Injury Zhengzhou University  Zhengzhou Henan China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,20]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140\u20106736(18)31878\u20106"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/s1474\u20104422(20)30364\u20101"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2023.1115031"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pneurobio.2019.03.003"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pneurobio.2010.08.001"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12035\u2010016\u20109785\u20106"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140\u20106736(19)30309\u20105"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.0000000000209714"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.4103\/1673\u20105374.268898"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1474\u20104422(04)00937\u20108"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.Neulet.2021.136297"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2019.06.038"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2017.03.010"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.2147\/IJN.S138410"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1177\/20417314231157004"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1049\/nbt2.12018"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2017.05.028"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11064\u2010022\u201003630\u2010z"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.colsurfb.2019.110545"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.3892\/or.2013.2418"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.nanolett.9b01900"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.Str.0000032302.91894.0f"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.121.319949"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.5853\/jos.2019.02236"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrneurol.2017.69"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1093\/brain\/awab367"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41572\u2010023\u201000424\u20107"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1093\/brain\/awz393"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2022.986469"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12975\u2010023\u201001225\u20106"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbi.2020.07.027"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.1002\/smtd.202400304"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2023.122277"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.7150\/thno.109440"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2022.844163"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.1080\/01616412.2021.1939487"},{"issue":"3","key":"e_1_2_11_38_1","first-page":"157","article-title":"Cancer\u2010Related Issues of CD147","volume":"7","author":"Weidle U. 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