{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T07:19:20Z","timestamp":1787901560243,"version":"build-2784847793"},"reference-count":53,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T00:00:00Z","timestamp":1786579200000},"content-version":"vor","delay-in-days":12,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100013254","name":"National College Students Innovation and Entrepreneurship Training Program","doi-asserted-by":"publisher","award":["202310343052"],"award-info":[{"award-number":["202310343052"]}],"id":[{"id":"10.13039\/501100013254","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["CNS Neurosci Ther"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Aims<\/jats:title>\n                    <jats:p>Spinal cord injury (SCI) can cause severe neurological dysfunction and the occurrence of chronic neuropathic pain, which can manifest as the occurrence of abnormal pain and hyperalgesia. Artemin (ARTN) is a member of the glial cell\u2010derived neurotrophic factor (GDNF) family ligand and can improve neural injury and regulate the occurrence of neuropathic pain. However, the process by which ARTN regulates inflammation and the sensitization of the dorsal horn of the spinal cord related to pain after SCI is still unclear.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>ARTN\u2010Fc fusion protein was constructed and administered intrathecally to mice after SCI. Motor recovery and pain\u2010related behaviors were evaluated using behavioral, gait, electrophysiological, paw withdrawal latency, and formalin\u2010induced Fos assays. Molecular changes were assessed by Western blotting, immunofluorescence, and immunohistochemistry. In\u00a0vitro, a BV2\u2013PC12 Transwell co\u2010culture system was used to examine the effect of ARTN\u2010Fc on activated microglia\u2010mediated neuronal injury.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>ARTN\u2010Fc treatment significantly improved motor recovery and reduced thermal hyperalgesia after SCI. Mechanistically, ARTN\u2010Fc promoted microglial M2 polarization, inhibited TLR4\/NF\u2010\u03baB activation, suppressed pro\u2010inflammatory cytokine expression, and attenuated NLRP3 inflammasome\/pyroptosis\u2010related signaling. In the spinal dorsal horn, ARTN\u2010Fc increased inhibitory GABAergic markers, including vGAT and GAD1, while reducing the excitatory marker vGluT2, suggesting altered inhibitory\/excitatory synaptic marker expression. In\u00a0vitro, ARTN\u2010Fc reduced neuronal apoptosis mediated by activated microglia.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Taken together, the results suggest that ARTN\u2010Fc is a potential therapeutic agent for SCI repair and neuropathic pain treatment by inhibiting the TLR4\/NF\u2010\u03baB pathway to suppress neuroinflammation and modulating inhibitory\/excitatory synaptic marker expression in the spinal dorsal horn.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/cns.71086","type":"journal-article","created":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T06:16:57Z","timestamp":1786688217000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Recombinant Artemin\u2010Fc Fusion Protein Attenuates\n                    <scp>TLR4<\/scp>\n                    \/\n                    <scp>NF<\/scp>\n                    \u2010\n                    <scp>\u03baB<\/scp>\n                    \u2010Associated Neuroinflammation and Modulates Inhibitory\/Excitatory Synaptic Marker Expression After Spinal Cord Injury"],"prefix":"10.1002","volume":"32","author":[{"given":"Wenjie","family":"Lu","sequence":"first","affiliation":[{"name":"Department of Orthopaedics, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University Wenzhou Medical University  Wenzhou China"},{"name":"State Key Laboratory of Macromolecular Drugs and Large\u2010Scale Preparation, School of Pharmaceutical Science Wenzhou Medical University  Wenzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yurong","family":"Tu","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University Wenzhou Medical University  Wenzhou China"},{"name":"State Key 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