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This study explored the pathways through which fatty acid\u2010binding protein 5 (\n                      <jats:italic>FABP5<\/jats:italic>\n                      ) promoted UVM progression.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Single\u2010cell RNA sequencing datasets were preprocessed by the Seurat package, and prognostic genes were screened using the survival package. A prognostic model was constructed via LASSO Cox regression, followed by Kaplan\u2013Meier (KM) survival analysis. To characterize the tumor immune microenvironment, we evaluated immune cell infiltration using ssGSEA, ESTIMATE, and CIBERSORT algorithms, while GSEA was applied to identify enriched pathways. Additionally, immunotherapy response was predicted by TIDE, and tumor mutation burden (TMB) was calculated using Maftools. For in\u00a0vivo validation, a nude mouse xenograft model was established, and Western blotting was performed on tumor tissues harvested from the xenograft assay.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      <jats:italic>SPON2<\/jats:italic>\n                      ,\n                      <jats:italic>MATK<\/jats:italic>\n                      , and\n                      <jats:italic>FABP5<\/jats:italic>\n                      were identified as hub genes in UVM and used to construct a prognostic model with a high AUC. Immune infiltration analysis revealed that high\u2010risk patients exhibited features of immune evasion. Enrichment analysis further revealed that cytotoxic T\u2010cell\u2010related genes (\n                      <jats:italic>MATK<\/jats:italic>\n                      and\n                      <jats:italic>FABP5<\/jats:italic>\n                      ) were enriched in the PI3K\/AKT\/mTOR pathway.\n                      <jats:italic>FABP5<\/jats:italic>\n                      was overexpressed in UVM cells, whereas\n                      <jats:italic>FABP5<\/jats:italic>\n                      silencing suppressed cell viability, proliferation, migration, and invasion, reduced MMP2 and MMP9 levels, promoted apoptosis, downregulated PI3K\/AKT\/mTOR pathway\u2010related molecules, and upregulated apoptosis\u2010related proteins. Notably, these effects could be reversed by the PI3K agonist 740Y\u2010P.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      <jats:italic>FABP5<\/jats:italic>\n                      promoted UVM cell proliferation and migration through the PI3K\/AKT\/mTOR pathway. Though the immune\u2010infiltration analysis was hypothesis\u2010generating, it may help identify patients likely to benefit from immunotherapy.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/cns.71112","type":"journal-article","created":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T09:04:21Z","timestamp":1788512661000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Fatty Acid\u2010Binding Protein 5 Potentially Regulates the Proliferation and Metastasis of Uveal Melanoma Cells Through the\n                    <scp>PI3K<\/scp>\n                    \/\n                    <scp>AKT<\/scp>\n                    \/\n                    <scp>mTOR<\/scp>\n                    Signaling Pathway"],"prefix":"10.1002","volume":"32","author":[{"given":"Yue","family":"Du","sequence":"first","affiliation":[{"name":"Ningbo Key Laboratory of Medical Research on Blinding Eye Diseases, Ningbo Eye Institute, Ningbo Eye Hospital Wenzhou Medical University  Ningbo China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xue","family":"Jiang","sequence":"additional","affiliation":[{"name":"Ningbo Key Laboratory of Medical Research on Blinding Eye Diseases, Ningbo Eye Institute, Ningbo Eye Hospital Wenzhou Medical University  Ningbo China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanyan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Ningbo Key Laboratory of Medical Research on Blinding Eye Diseases, Ningbo Eye Institute, Ningbo Eye Hospital Wenzhou Medical University  Ningbo China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-4518-7614","authenticated-orcid":false,"given":"Jianing","family":"Ying","sequence":"additional","affiliation":[{"name":"Ningbo Key Laboratory of Medical Research on Blinding Eye Diseases, Ningbo Eye Institute, Ningbo Eye Hospital Wenzhou Medical University  Ningbo China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9369-3998","authenticated-orcid":false,"given":"Quanyong","family":"Yi","sequence":"additional","affiliation":[{"name":"Ningbo Key Laboratory of Medical Research on Blinding Eye Diseases, Ningbo Eye Institute, Ningbo Eye Hospital Wenzhou Medical University  Ningbo China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,4]]},"reference":[{"key":"e_1_2_14_2_1","doi-asserted-by":"publisher","DOI":"10.2174\/0109298665341953240926041613"},{"key":"e_1_2_14_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-16276-8"},{"key":"e_1_2_14_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/curroncol31010002"},{"key":"e_1_2_14_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.apjo.2024.100060"},{"key":"e_1_2_14_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcancer.2020.05.003"},{"key":"e_1_2_14_7_1","doi-asserted-by":"publisher","DOI":"10.3233\/CBM-210427"},{"key":"e_1_2_14_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-14256-1"},{"key":"e_1_2_14_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.preteyeres.2022.101041"},{"key":"e_1_2_14_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41417-022-00442-9"},{"key":"e_1_2_14_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(23)00521-4"},{"key":"e_1_2_14_12_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa2304753"},{"key":"e_1_2_14_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phymed.2024.156316"},{"key":"e_1_2_14_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joca.2020.02.027"},{"key":"e_1_2_14_15_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms24054522"},{"key":"e_1_2_14_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.drudis.2023.103628"},{"key":"e_1_2_14_17_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2407-14-724"},{"key":"e_1_2_14_18_1","doi-asserted-by":"publisher","DOI":"10.7150\/thno.71456"},{"key":"e_1_2_14_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/cam4.1020"},{"key":"e_1_2_14_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcmm.16815"},{"key":"e_1_2_14_21_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12967-023-04578-6"},{"key":"e_1_2_14_22_1","doi-asserted-by":"publisher","DOI":"10.7150\/ijbs.96831"},{"key":"e_1_2_14_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2024.09.029"},{"issue":"10","key":"e_1_2_14_24_1","article-title":"FABP5 as a Novel Molecular Target in Prostate Cancer","volume":"18","author":"O'Sullivan S. 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