{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T17:57:47Z","timestamp":1790618267177,"version":"4.1.0"},"reference-count":56,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T00:00:00Z","timestamp":1789689600000},"content-version":"vor","delay-in-days":17,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["CNS Neurosci Ther"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Aims<\/jats:title>\n                    <jats:p>The anterior cingulate cortex (ACC) is widely recognized as a key brain region in modulating itch and itch\u2010associated behaviors. Though neuronal mechanisms are increasingly clarified, the role of ACC astrocytes remains poorly understood.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>By employing a diphenylcyclopropenone (DCP)\u2010induced chronic itch mouse model, we assessed scratching behavior and negative emotions via behavioral tests. Astrocytic morphology was examined through immunofluorescence staining and sholl analysis. Fiber photometry was used to record calcium signals of ACC astrocytes and CaMKII\u2010positive neurons. Chemogenetic manipulation was applied to modulate astrocyte activity. Gliotransmitter quantification was implemented to detect the gliotransmitter release in the ACC.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>DCP induced robust scratching responses and concomitant anxiety\u2010 and depression\u2010like behaviors. ACC astrocytes exhibited morphological activation and elevated calcium responses. Chemogenetic activation of ACC astrocytes alleviated DCP\u2010induced scratching behavior and negative emotions, and enhanced calcium activity of local CaMKII\u2010positive neurons. Conversely, chemogenetic inhibition of ACC astrocytes reduced such neuronal calcium activity. D\u2010serine and ATP levels were reduced in DCP\u2010induced chronic itch, while chemogenetic activation of astrocytes significantly elevated them.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>ACC astrocytes play a critical role in regulating chronic itch and comorbid anxiety\u2010depression by modulating CaMKII\u2010positive neuronal activity, providing a potential therapeutic target for intractable chronic itch in clinical settings.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/cns.71165","type":"journal-article","created":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T14:38:47Z","timestamp":1789742327000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Anterior Cingulate Cortex Astrocytes Regulate Chronic Itch and Comorbid Negative Emotional Disorders in 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