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Disorders in these processes have been associated with fibrosis, developmental malformations, cancer, and other diseases. This unit describes a method for preparing a three\u2010dimensional matrix derived from fibroblastic cells; the matrix is three\u2010dimensional, cell and debris free, and attached to a two\u2010dimensional culture surface. Cell adhesion and spreading are normal on these matrices. This matrix can also be compressed into a two\u2010dimensional matrix and solubilized to study the matrix biochemically. \u00a9 2016 by John Wiley &amp; Sons, Inc.<\/jats:p>","DOI":"10.1002\/cpcb.2","type":"journal-article","created":{"date-parts":[[2016,6,1]],"date-time":"2016-06-01T11:41:59Z","timestamp":1464781319000},"source":"Crossref","is-referenced-by-count":156,"title":["Preparation of Extracellular Matrices Produced by Cultured and Primary Fibroblasts"],"prefix":"10.1002","volume":"71","author":[{"given":"Janusz","family":"Franco\u2010Barraza","sequence":"first","affiliation":[{"name":"Fox Chase Cancer Center  Philadelphia Pennsylvania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dorothy A.","family":"Beacham","sequence":"additional","affiliation":[{"name":"Fox Chase Cancer Center  Philadelphia Pennsylvania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael D.","family":"Amatangelo","sequence":"additional","affiliation":[{"name":"Fox Chase Cancer Center  Philadelphia Pennsylvania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edna","family":"Cukierman","sequence":"additional","affiliation":[{"name":"Fox Chase Cancer Center  Philadelphia Pennsylvania"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,6]]},"reference":[{"key":"e_1_2_18_1_1_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0002\u20109440(10)62991\u20104"},{"key":"e_1_2_18_1_2_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.cb.03.110187.001143"},{"key":"e_1_2_18_1_3_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.cellbio.12.1.463"},{"key":"e_1_2_18_1_4_1","first-page":"79","article-title":"Cell migration analyses within fibroblast\u2010derived 3\u2010D matrices","volume":"294","author":"Cukierman E.","year":"2005","journal-title":"Methods Mol. 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