{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:38:40Z","timestamp":1786977520155,"version":"build-2736575974"},"reference-count":37,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2018,8,31]],"date-time":"2018-08-31T00:00:00Z","timestamp":1535673600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003246","name":"Nederlandse Organisatie voor Wetenschappelijk Onderzoek","doi-asserted-by":"publisher","award":["CANCER\u2010ID 14195"],"award-info":[{"award-number":["CANCER\u2010ID 14195"]}],"id":[{"id":"10.13039\/501100003246","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003246","name":"Nederlandse Organisatie voor Wetenschappelijk Onderzoek","doi-asserted-by":"publisher","award":["VENI 13681"],"award-info":[{"award-number":["VENI 13681"]}],"id":[{"id":"10.13039\/501100003246","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003246","name":"Nederlandse Organisatie voor Wetenschappelijk Onderzoek","doi-asserted-by":"publisher","award":["VENI 15924"],"award-info":[{"award-number":["VENI 15924"]}],"id":[{"id":"10.13039\/501100003246","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["currentprotocols.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["CP Cytometry"],"published-print":{"date-parts":[[2018,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Flow cytometry is commonly used to investigate the potential for extracellular vesicles (EVs) to be biomarkers of disease. A typical flow cytometer detects fluorescence and scatter intensities of single EVs in arbitrary units. These arbitrary units complicate data interpretation and data comparison between different flow cytometers. For example, comparison of detected EV concentrations requires knowledge of the detectable EV sizes. Using Mie theory and knowledge of the optical configuration of the flow cytometer, EV size can be derived from the scatter intensity for a given EV refractive index. Here, a protocol is described to derive the size of EVs and other nanoparticles from the scatter intensity. The resulting size distribution allows the comparison of data between flow cytometers, which is a prerequisite for clinical application of EVs as biomarkers and may advance other fields where sizing of nanoparticles is essential. \u00a9 2018 by John Wiley &amp; Sons, Inc.<\/jats:p>","DOI":"10.1002\/cpcy.43","type":"journal-article","created":{"date-parts":[[2018,8,31]],"date-time":"2018-08-31T07:45:20Z","timestamp":1535701520000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":61,"title":["Deriving Extracellular Vesicle Size From Scatter Intensities Measured by Flow Cytometry"],"prefix":"10.1002","volume":"86","author":[{"given":"Leonie","family":"de Rond","sequence":"first","affiliation":[{"name":"Amsterdam UMC, University of Amsterdam, Biomedical Engineering and Physics  Meibergdreef 9 Amsterdam The Netherlands"},{"name":"Amsterdam UMC, University of Amsterdam, Laboratory Experimental Clinical Chemistry  Meibergdreef 9 Amsterdam The Netherlands"},{"name":"Amsterdam UMC, University of Amsterdam, Vesicle Observation Center  Meibergdreef 9 Amsterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frank A. W.","family":"Coumans","sequence":"additional","affiliation":[{"name":"Amsterdam UMC, University of Amsterdam, Biomedical Engineering and Physics  Meibergdreef 9 Amsterdam The Netherlands"},{"name":"Amsterdam UMC, University of Amsterdam, Laboratory Experimental Clinical Chemistry  Meibergdreef 9 Amsterdam The Netherlands"},{"name":"Amsterdam UMC, University of Amsterdam, Vesicle Observation Center  Meibergdreef 9 Amsterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rienk","family":"Nieuwland","sequence":"additional","affiliation":[{"name":"Amsterdam UMC, University of Amsterdam, Laboratory Experimental Clinical Chemistry  Meibergdreef 9 Amsterdam The Netherlands"},{"name":"Amsterdam UMC, University of Amsterdam, Vesicle Observation Center  Meibergdreef 9 Amsterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ton G.","family":"van Leeuwen","sequence":"additional","affiliation":[{"name":"Amsterdam UMC, University of Amsterdam, Biomedical Engineering and Physics  Meibergdreef 9 Amsterdam The Netherlands"},{"name":"Amsterdam UMC, University of Amsterdam, Vesicle Observation Center  Meibergdreef 9 Amsterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edwin","family":"van der Pol","sequence":"additional","affiliation":[{"name":"Amsterdam UMC, University of Amsterdam, Biomedical Engineering and Physics  Meibergdreef 9 Amsterdam The Netherlands"},{"name":"Amsterdam UMC, University of Amsterdam, Laboratory Experimental Clinical Chemistry  Meibergdreef 9 Amsterdam The Netherlands"},{"name":"Amsterdam UMC, University of Amsterdam, Vesicle Observation Center  Meibergdreef 9 Amsterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,8,31]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/jth.12767"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/jth.12554"},{"key":"e_1_2_7_4_1","volume-title":"Absorption and scattering of light by small particles","author":"Bohren C. 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