{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T05:20:49Z","timestamp":1787721649897,"version":"build-2784847793"},"reference-count":101,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T00:00:00Z","timestamp":1782777600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T00:00:00Z","timestamp":1782777600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100004410","name":"T\u00fcrkiye Bilimsel ve Teknolojik Ara\u015ft\u0131rma Kurumu","doi-asserted-by":"publisher","award":["115S381"],"award-info":[{"award-number":["115S381"]}],"id":[{"id":"10.13039\/501100004410","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Comprehensive Physiology"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Sensory neurons innervating the heart transmit chemical and mechanical cues to the central nervous system via the dorsal root ganglion (DRG) and nodose ganglion (NG). Despite their importance in cardiac pain and cardiovascular reflexes, the molecular and functional properties of heart\u2010specific sensory (HS) neurons remain elusive. Here, DRG\n                    <jats:sup>HS<\/jats:sup>\n                    and NG\n                    <jats:sup>HS<\/jats:sup>\n                    neurons innervating the heart were FACS purified using a retrograde\u00a0labeling strategy with Di\u20108\u2010ANEPPQ, then characterized molecularly by bulk RNA sequencing or evaluated functionally in cocultures with neonatal cardiomyocytes. DRG\n                    <jats:sup>HS<\/jats:sup>\n                    and NG\n                    <jats:sup>HS<\/jats:sup>\n                    neurons formed functional connections with neonatal cardiomyocytes as demonstrated by immunolabeling, electron microscopy, and optogenetic manipulation. Functionally coupled DRG\n                    <jats:sup>HS<\/jats:sup>\n                    neurons exhibited enhanced spontaneous and evoked Ca\n                    <jats:sup>2+<\/jats:sup>\n                    activity in response to optogenetic and chemical stimulation, indicating dynamic\u00a0neuro\u2010cardiac communication. Global RNA sequencing analysis revealed that DRG\n                    <jats:sup>HS<\/jats:sup>\n                    and NG\n                    <jats:sup>HS<\/jats:sup>\n                    neurons exhibited distinct transcriptomic profiles, including enrichment of transcripts encoding ion channels and G protein\u2010coupled receptors, compared with their respective total populations. In DRG\n                    <jats:sup>HS<\/jats:sup>\n                    tissue, these included\n                    <jats:italic>Scn10a<\/jats:italic>\n                    ,\n                    <jats:italic>P2xr2<\/jats:italic>\n                    , and\n                    <jats:italic>Mrgprd<\/jats:italic>\n                    , whereas NG\n                    <jats:sup>HS<\/jats:sup>\n                    neurons preferentially expressed\n                    <jats:italic>P2xr2<\/jats:italic>\n                    ,\n                    <jats:italic>Ptgdr<\/jats:italic>\n                    , and\n                    <jats:italic>Cckar<\/jats:italic>\n                    , collectively supporting a combination of molecular signatures including nociceptors. Collectively, these findings define molecularly distinct sensory pathways connecting the heart and the sensory nervous system, providing mechanistic insights and highlighting potential targets for modulation of cardiovascular reflexes and hemostasis.\n                  <\/jats:p>","DOI":"10.1002\/cph4.70203","type":"journal-article","created":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T08:13:45Z","timestamp":1782893625000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Heart\u2010Specific Spinal and Vagal Afferents: Transcriptomic Signatures and Optogenetically Modulated Functional Coupling With Cardiomyocytes"],"prefix":"10.1002","volume":"16","author":[{"given":"T.","family":"Akgul Caglar","sequence":"first","affiliation":[{"name":"Research Institute for Health Sciences and Technologies (SABITA), Istanbul Medipol University  Istanbul T\u00fcrkiye"},{"name":"Neuroscience Program, Institute of Health Sciences Istanbul Medipol University  Istanbul T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4610-9920","authenticated-orcid":false,"given":"Y. E.","family":"Kazci","sequence":"additional","affiliation":[{"name":"Research Institute for Health Sciences and Technologies (SABITA), Istanbul Medipol University  Istanbul T\u00fcrkiye"},{"name":"Neuroscience Program, Institute of Health Sciences Istanbul Medipol University  Istanbul T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Z. B.","family":"Durdu","sequence":"additional","affiliation":[{"name":"Research Institute for Health Sciences and Technologies (SABITA), Istanbul Medipol University  Istanbul T\u00fcrkiye"},{"name":"Medical Biology and Genetics Program, Institute of Health Sciences Istanbul Medipol University  Istanbul T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Sahoglu Goktas","sequence":"additional","affiliation":[{"name":"Research Institute for Health Sciences and Technologies (SABITA), Istanbul Medipol University  Istanbul T\u00fcrkiye"},{"name":"Neuroscience Program, Institute of Health Sciences Istanbul Medipol University  Istanbul T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Bay","sequence":"additional","affiliation":[{"name":"Research Institute for Health Sciences and Technologies (SABITA), Istanbul Medipol University  Istanbul T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4681-9703","authenticated-orcid":false,"given":"E.","family":"Vatandaslar","sequence":"additional","affiliation":[{"name":"Research Institute for Health Sciences and Technologies (SABITA), Istanbul Medipol University  Istanbul T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. U.","family":"Turhan","sequence":"additional","affiliation":[{"name":"Research Institute for Health Sciences and Technologies (SABITA), Istanbul Medipol University  Istanbul T\u00fcrkiye"},{"name":"Department of Cardiovascular Surgery, Haseki Training and Research Hospital University of Health Sciences  Istanbul T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G.","family":"Ozturk","sequence":"additional","affiliation":[{"name":"Research Institute for Health Sciences and Technologies (SABITA), Istanbul Medipol University  Istanbul T\u00fcrkiye"},{"name":"Department of Physiology, School of Medicine Bolu Abant Izzet Baysal University  Bolu T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7199-583X","authenticated-orcid":false,"given":"E.","family":"Cagavi","sequence":"additional","affiliation":[{"name":"Research Institute for Health Sciences and Technologies (SABITA), Istanbul Medipol University  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