{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T06:31:01Z","timestamp":1791268261768,"version":"4.1.0"},"reference-count":20,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2009,3,2]],"date-time":"2009-03-02T00:00:00Z","timestamp":1235952000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["chemistry-europe.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["ChemPhysChem"],"published-print":{"date-parts":[[2009,3,23]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:bold>Selective DNA detection:<\/jats:bold> The fluorescence, from stable cationic QDs, is quenched by 90% on complexation with modified DNA molecules. The QD\u2013DNA probe is capable of detecting pathogenic DNA fragments at concentrations as low as 200 n<jats:sc>M<\/jats:sc> in solution and shows selective fluorescence recovery in the presence of target DNA (see spectrum c in figure) vs noncomplementary DNA (spectrum d).<jats:boxed-text content-type=\"graphic\" position=\"anchor\"><jats:graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" mimetype=\"image\/gif\" position=\"anchor\" specific-use=\"enlarged-web-image\" xlink:href=\"graphic\/mcontent.gif\"><jats:alt-text>magnified image<\/jats:alt-text><\/jats:graphic><\/jats:boxed-text><\/jats:p><jats:p><jats:italic>Novel QD\u2013DNA complexes are prepared by simple electrostatic interaction between pegylated amine\u2010functionalized CdSe\/ZnS quantum dots (QDs) and DNA. The cationic nature of the amine functionality on the QD surface allows for formation of an electrostatic complex with negatively charged DNA. The presence of polyethylene glycol (PEG5000) molecules on the QD leads to enhanced stability and decreased nonspecific adsorption of DNA on the QD surface. Unlike assembly of QD\u2013DNA based on hydrogen bonding, the present QD probes tend to be more strongly stabilized during the hybridization process by increasing the overall negative charges. In addition, the DNA loading efficiency can be modulated by changing the pH of the reaction medium. The fluorescence of the QD is quenched up to 90\u2009% by complexation with 5\u2032\u2010TAMRA\u2010modified oligonucleotide (TAMRA=carboxytetramethylrhodamine) through fluorescence resonance energy transfer (FRET). With the FRET pair we selected, the<\/jats:italic> R<jats:sub><jats:italic>0<\/jats:italic><\/jats:sub> <jats:italic>value was calculated to be 5.5 nm and<\/jats:italic> r <jats:italic>is about 5 nm. This quenching of QD fluorescence is then reversed on binding of unlabeled target DNA. The maximum recovery of QD fluorescence is 60\u2009%. The QD\u2013DNA probe (5DNA\/QD) exhibits selective photoluminescence (PL) recovery in the presence of target oligonucleotide with a PL ratio of 3 for complementary versus noncomplementary. The present QD\u2013DNA probes also show the capability to detect the synthetic 100\u2010mer oligonucleotide derived from H5N1 influenza virus when present at concentrations as low as 200 n<jats:sc>M<\/jats:sc> in the solution.<\/jats:italic><\/jats:p>","DOI":"10.1002\/cphc.200800504","type":"journal-article","created":{"date-parts":[[2009,3,2]],"date-time":"2009-03-02T09:41:29Z","timestamp":1235986889000},"page":"806-811","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":70,"title":["Positively Charged Compact Quantum Dot\u2013DNA Complexes for Detection of Nucleic Acids"],"prefix":"10.1002","volume":"10","author":[{"given":"Junghan","family":"Lee","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Youngseon","family":"Choi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junwon","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eunjung","family":"Park","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rita","family":"Song","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2009,3,17]]},"reference":[{"key":"e_1_2_6_1_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt927"},{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1104274"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1021\/jp054874p"},{"key":"e_1_2_6_4_2","unstructured":"\u00a0"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.3390\/s6080925"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/cphc.200500217"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmat1390"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja0125570"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1039\/b500664c"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja0685452"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1021\/la703583u"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1039\/b801317a"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1039\/b719434j"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1021\/cm034081k"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1021\/la062217y"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1039\/b508911e"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja045676z"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1002\/ange.200502279"},{"key":"e_1_2_6_18_3","doi-asserted-by":"publisher","DOI":"10.1002\/anie.200502279"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja003633m"}],"container-title":["ChemPhysChem"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcphc.200800504","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/chemistry-europe.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cphc.200800504","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,14]],"date-time":"2025-10-14T04:31:08Z","timestamp":1760416268000},"score":1,"resource":{"primary":{"URL":"https:\/\/chemistry-europe.onlinelibrary.wiley.com\/doi\/10.1002\/cphc.200800504"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,3,17]]},"references-count":20,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2009,3,23]]}},"alternative-id":["10.1002\/cphc.200800504"],"URL":"https:\/\/doi.org\/10.1002\/cphc.200800504","archive":["Portico"],"relation":{},"ISSN":["1439-4235","1439-7641"],"issn-type":[{"value":"1439-4235","type":"print"},{"value":"1439-7641","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,3,17]]},"assertion":[{"value":"2008-08-05","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2009-03-17","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}