{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T08:08:54Z","timestamp":1791187734092,"version":"4.1.0"},"reference-count":33,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2012,1,20]],"date-time":"2012-01-20T00:00:00Z","timestamp":1327017600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["chemistry-europe.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["ChemPhysChem"],"published-print":{"date-parts":[[2012,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Single\u2010molecule super\u2010resolution imaging provides a non\u2010invasive method for nanometer\u2010scale imaging and is ideally suited to investigations of quasi\u2010static structures within live cells. Here, we extend the ability to image subcellular features within bacteria cells to three dimensions based on the introduction of a cylindrical lens in the imaging pathway. We investigate the midplane protein FtsZ in <jats:italic>Caulobacter crescentus<\/jats:italic> with super\u2010resolution imaging based on fluorescent\u2010protein photoswitching and the natural polymerization\/depolymerization dynamics of FtsZ associated with the Z\u2010ring. We quantify these dynamics and determine the FtsZ depolymerization time to be &lt;100 ms. We image the Z\u2010ring in live and fixed <jats:italic>C. crescentus<\/jats:italic> cells at different stages of the cell cycle and find that the FtsZ superstructure is dynamic with the cell cycle, forming an open shape during the stalked stage and a dense focus during the pre\u2010divisional stage.<\/jats:p>","DOI":"10.1002\/cphc.201100686","type":"journal-article","created":{"date-parts":[[2012,1,20]],"date-time":"2012-01-20T02:47:51Z","timestamp":1327027671000},"page":"1007-1012","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":93,"title":["Three\u2010Dimensional Super\u2010Resolution Imaging of the Midplane Protein FtsZ in Live <i>Caulobacter crescentus<\/i> Cells Using Astigmatism"],"prefix":"10.1002","volume":"13","author":[{"given":"Julie S.","family":"Biteen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erin D.","family":"Goley","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucy","family":"Shapiro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W. 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