{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T06:39:56Z","timestamp":1790145596524,"version":"4.0.1"},"reference-count":53,"publisher":"Wiley","issue":"18","license":[{"start":{"date-parts":[[2016,7,14]],"date-time":"2016-07-14T00:00:00Z","timestamp":1468454400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"DST","award":["IR\/S1\/CU 02\/2009"],"award-info":[{"award-number":["IR\/S1\/CU 02\/2009"]}]},{"name":"J. C. 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From the emission maximum <jats:inline-graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"graphic\/cphc201600425-math-0001.png\" xlink:title=\"urn:x-wiley:14394235:media:cphc201600425:cphc201600425-math-0001\"\/>\n of the ER\u2010Tracker dye, polarity (i.e. dielectric constant, <jats:italic>\u03f5<\/jats:italic>) in the ER region of the cells (\u2248500\u2005nm in WI38 and \u2248510\u2005nm in A549) is estimated to be similar to that of chloroform (<jats:inline-graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"graphic\/cphc201600425-math-0002.png\" xlink:title=\"urn:x-wiley:14394235:media:cphc201600425:cphc201600425-math-0002\"\/>\n=506\u2005nm, <jats:italic>\u03f5<\/jats:italic>\u22485). The red shift by 10\u2005nm in <jats:inline-graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"graphic\/cphc201600425-math-0003.png\" xlink:title=\"urn:x-wiley:14394235:media:cphc201600425:cphc201600425-math-0003\"\/>\n in the cancer cell (A549) suggests a slightly higher polarity compared to the non\u2010cancer cell (WI38). The fluorescence intensity of the ER\u2010Tracker dye exhibits prolonged intermittent oscillations on a timescale of 2\u20136\u2005seconds for the cancer cell (A549). For the non\u2010cancer cell (WI38), such fluorescence oscillations are much less prominent. The marked fluorescence intensity oscillations in the cancer cell are attributed to enhanced calcium oscillations. The average solvent relaxation time (&lt;<jats:italic>\u03c4<\/jats:italic><jats:sub>s<\/jats:sub>&gt;) of the ER region in the lung cancer cell (A549, 250\u00b150\u2005ps) is about four times faster than that in the non\u2010cancer cell (WI38, 1000\u00b150\u2005ps).<\/jats:p>","DOI":"10.1002\/cphc.201600425","type":"journal-article","created":{"date-parts":[[2016,6,1]],"date-time":"2016-06-01T02:53:00Z","timestamp":1464749580000},"page":"2818-2823","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["Fluorescence Dynamics in the Endoplasmic Reticulum of a Live Cell: Time\u2010Resolved Confocal Microscopy"],"prefix":"10.1002","volume":"17","author":[{"given":"Shirsendu","family":"Ghosh","sequence":"first","affiliation":[{"name":"Department of Physical Chemistry Indian Association for the Cultivation of Science, Jadavpur  Kolkata 700 032 India), Fax: 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