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Herein, we show that the steady\u2010state emission spectra obtained from photoluminescence (PL) simultaneously capture both the tyrosine derivative (tyrosinate) and the structure\u2010specific intrinsic fluorescence during the aggregation of A\u03b2 and hIAPP. We observe multiple peaks in the emission spectra which exist for structure\u2010specific intrinsic fluorescence, and use the second derivative UV\u2010Vis spectra and the shift in the tyrosine peak as a quantitative measure of the dissimilitude in the electronic states and the fibril growth. We further applied these techniques to detect the static electric field (0, 40, 120, 200\u2005V\/cm) induced promotion and inhibition of fibrillation in A\u03b2, hIAPP and their electric field dependent role in the fibrillation of A\u03b2\u2009:\u2009hIAPP(1\u2009:\u20091). The results were corroborated by field\u2010emission scanning electron microscopy (FESEM), and the determinations of secondary structures by Fourier transform infrared spectroscopy (FTIR). The results indicate that the emission spectrum can be used as a sensor to detect the presence of fibrils; hence for screening potential inhibitors of amyloid fibrillation.<\/jats:p>","DOI":"10.1002\/cphc.202000587","type":"journal-article","created":{"date-parts":[[2020,10,2]],"date-time":"2020-10-02T04:40:41Z","timestamp":1601613641000},"page":"2585-2598","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Simultaneous Detection of Tyrosine and Structure\u2010Specific Intrinsic Fluorescence in the Fibrillation of Alzheimer's Associated Peptides"],"prefix":"10.1002","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8132-903X","authenticated-orcid":false,"given":"Anurag","family":"Singh","sequence":"first","affiliation":[{"name":"Biophysics and Soft Matter Laboratory Department of Physics Indian Institute of Technology  Kharagpur 721302 India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6177-9052","authenticated-orcid":false,"given":"Suparna","family":"Khatun","sequence":"additional","affiliation":[{"name":"Biophysics and Soft Matter Laboratory Department of Physics Indian Institute of Technology  Kharagpur 721302 India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2013-8421","authenticated-orcid":false,"given":"Amar","family":"Nath Gupta","sequence":"additional","affiliation":[{"name":"Biophysics and Soft Matter Laboratory Department of Physics Indian Institute of Technology  Kharagpur 721302 India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,11,12]]},"reference":[{"key":"e_1_2_7_1_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jalz.2019.01.010"},{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.2000.3051"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1742-4658.2006.05367.x"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/aps.2011.14"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1097\/00060793-199708000-00006"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/ana.23956"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuropharm.2017.12.016"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajpath.2010.11.022"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10495-011-0656-3"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamem.2007.03.015"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpj.2009.07.064"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1039\/c0cp02652b"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbic.201300103"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2010.03.005"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1088\/2050-6120\/1\/1\/015006"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsomega.8b02408"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jpclett.5b01285"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0702843104"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1039\/c3an36798c"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.abb.2004.05.007"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.5b11012"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2019.04.167"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1039\/C9CP03238J"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1021\/jp807896g"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1063\/1.4979866"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1039\/C3SM52653D"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpj.2010.07.014"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi301525e"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1063\/1.4941108"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1063\/1.1661347"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.6028\/NBS.CIRC.514"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1103\/PhysRevE.101.062413"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1039\/C9SM02463H"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751-1097.1977.tb07527.x"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jpcb.7b10765"},{"key":"e_1_2_7_36_1","volume-title":"Principles of fluorescence spectroscopy","author":"Lakowicz J. 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