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The cause for this reduced photoconversion is not yet clear, although in the related NpR6012g4 dark\u2010state structural heterogeneity of a paramount Trp residue has been proposed to cause the formation of nonproductive subpopulation. However, there is no such information on the equivalent residue in slr1393g3, W496. Here we use solid\u2010state NMR to explore all possible sidechain rotamers of this Trp residue and their local interactions at the atomic level. The indole nitrogen (N<jats:italic>\u03f5<\/jats:italic>1) is used as an NMR probe, achieved by site\u2010specific <jats:sup>15<\/jats:sup>N\u2010indole labeling of a quadruply Trp\u2010deleted variant and trehalose vitrification technique. The data reveal a set of seven indole rotamers of W496 with four distinct environments for the N<jats:italic>\u03f5<\/jats:italic>1\u2010H group. Only a minority population of 20\u2009% is found to retain the \u03c0\u2010stacking and hydrogen\u2010bonding interactions with the chromophore in the dark state that has been assigned to account for complete forward photoconversion. Our results demonstrate the direct role of W496 in modulating the forward quantum yield of slr1393g3 via rearrangement of its sidechain rotameric conformations.<\/jats:p>","DOI":"10.1002\/cphc.202400453","type":"journal-article","created":{"date-parts":[[2024,10,9]],"date-time":"2024-10-09T11:24:58Z","timestamp":1728473098000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Rotameric Heterogeneity of Conserved Tryptophan Is Responsible for Reduced Photochemical Quantum Yield in Cyanobacteriochrome Slr1393g3"],"prefix":"10.1002","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4265-3726","authenticated-orcid":false,"given":"Lisa","family":"K\u00f6hler","sequence":"first","affiliation":[{"name":"Institut f\u00fcr Analytische Chemie Universit\u00e4t Leipzig  04103 Leipzig 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