{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,14]],"date-time":"2025-10-14T01:03:26Z","timestamp":1760403806673,"version":"build-2065373602"},"reference-count":24,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2025,3,11]],"date-time":"2025-03-11T00:00:00Z","timestamp":1741651200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["22274034"],"award-info":[{"award-number":["22274034"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012226","name":"Fundamental Research Funds for the Central Universities","doi-asserted-by":"publisher","award":["JZ2022HGPB0307"],"award-info":[{"award-number":["JZ2022HGPB0307"]}],"id":[{"id":"10.13039\/501100012226","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["chemistry-europe.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["ChemPhysChem"],"published-print":{"date-parts":[[2025,5,19]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Spherical nucleic acids (SNAs), with their densely packed nucleic acid shells and programmable functionalities, have become indispensable in nanomedicine and biosensing. Developed synthesis methods, including salt aging, pH modulation, freeze\u2010thaw cycling, n\u2010butanol dehydration, evaporation drying, and microwave heating, have enabled foundational advances but are constrained by slow kinetics, compromised structural uniformity and especially harsh reaction conditions, making them unsuitable for in\u2005situ tracking of biological events. This concept article introduces\u00a0acoustic levitation synthesis\u00a0as a groundbreaking alternative, uniquely addressing these limitations through a rapid, green, and highly controllable process. By leveraging non\u2010contact acoustic radiation forces, this method enables the synthesis of ultrahigh\u2010density SNAs within minutes under ambient conditions, eliminating the need for toxic reagents or energy\u2010intensive steps. The resulting SNAs exhibit superior homogeneity and stability compared to conventional approaches. We critically evaluate the conceptual novelty and limitations of this technique. Potential applications in surface\u2010enhanced Raman spectroscopy (SERS) and targeted therapeutics are highlighted, positioning acoustic levitation as a transformative tool for next\u2010generation nanobiotechnology.<\/jats:p>","DOI":"10.1002\/cphc.202401008","type":"journal-article","created":{"date-parts":[[2025,3,11]],"date-time":"2025-03-11T19:19:54Z","timestamp":1741720794000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Spherical Nucleic Acids Meet Acoustic Levitation: A Breakthrough in Synthesis and Application"],"prefix":"10.1002","volume":"26","author":[{"given":"Leiming","family":"Chu","sequence":"first","affiliation":[{"name":"Anhui Provincial International Science and Technology Cooperation Base for Major Metabolic Diseases and Nutritional Interventions China Light Industry Key Laboratory of Meat Microbial Control and Utilization School of Food and Biological Engineering Engineering Research Center of Bio-process Ministry of Education Hefei University of Technology  Hefei 230601 P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ning","family":"Li","sequence":"additional","affiliation":[{"name":"Anhui Provincial International Science and Technology Cooperation Base for Major Metabolic Diseases and Nutritional Interventions China Light Industry Key Laboratory of Meat Microbial Control and Utilization School of Food and Biological Engineering Engineering Research Center of Bio-process Ministry of Education Hefei University of Technology  Hefei 230601 P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Heng","family":"Gao","sequence":"additional","affiliation":[{"name":"Anhui Provincial International Science and Technology Cooperation Base for Major Metabolic Diseases and Nutritional Interventions China Light Industry Key Laboratory of Meat Microbial Control and Utilization School of Food and Biological Engineering Engineering Research Center of Bio-process Ministry of Education Hefei University of Technology  Hefei 230601 P. R. 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