{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T11:16:36Z","timestamp":1789384596686,"version":"build-2803163510"},"reference-count":14,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2017,4,6]],"date-time":"2017-04-06T00:00:00Z","timestamp":1491436800000},"content-version":"vor","delay-in-days":5,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["CP Human Genetics"],"published-print":{"date-parts":[[2017,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Reliable measurement of creatinine is necessary to assess kidney function, and also to quantitate drug levels and diagnostic compounds in urine samples. The most commonly used methods are based on the Jaffe principal of alkaline creatinine\u2010picric acid complex color formation. However, other compounds commonly found in serum and urine may interfere with Jaffe creatinine measurements. Therefore, many laboratories have made modifications to the basic method to remove or account for these interfering substances. This appendix will summarize the basic Jaffe method, as well as a modified, automated version. Also described is a high performance liquid chromatography (HPLC) method that separates creatinine from contaminants prior to direct quantification by UV absorption. Lastly, a liquid chromatography\u2010tandem mass spectrometry (LC\u2010MS\/MS) method is described that uses stable isotope dilution to reliably quantify creatinine in any sample. This last approach has been recommended by experts in the field as a means to standardize all quantitative creatinine methods against an accepted reference. \u00a9 2017 by John Wiley &amp; Sons, Inc.<\/jats:p>","DOI":"10.1002\/cphg.38","type":"journal-article","created":{"date-parts":[[2017,4,6]],"date-time":"2017-04-06T19:33:58Z","timestamp":1491507238000},"source":"Crossref","is-referenced-by-count":36,"title":["Methods for Quantitative Creatinine Determination"],"prefix":"10.1002","volume":"93","author":[{"given":"John F.","family":"Moore","sequence":"first","affiliation":[{"name":"Department of Genetics, University of Alabama at Birmingham  Birmingham Alabama"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. Daniel","family":"Sharer","sequence":"additional","affiliation":[{"name":"Department of Genetics, University of Alabama at Birmingham  Birmingham Alabama"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,4,6]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0009\u20108981(69)90278\u20102"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/ndt\/gfl374"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/clinchem\/17.8.696"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)88386-7"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0009\u20108981(73)90466\u2010X"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1515\/bchm1.1886.10.5.391"},{"key":"e_1_2_7_8_1","first-page":"716","volume-title":"Biochemistry, the molecular basis of cell structure and function 2nd edition","author":"Lehninger A.","year":"1975"},{"key":"e_1_2_7_9_1","first-page":"173","article-title":"Measurement of serum creatinine \u2010 current status and future goals","volume":"27","author":"Peake M.","year":"2006","journal-title":"The Clinical Biochemist"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1097\/00004872\u2010199917030\u201000002"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra054415"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.ki.5002033"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2003.024141"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0306\u20104522(02)00088\u2010X"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/0471142905.hg1703s54"}],"container-title":["Current Protocols in Human Genetics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcphg.38","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cphg.38","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/cphg.38","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/currentprotocols.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cphg.38","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,20]],"date-time":"2023-08-20T05:09:08Z","timestamp":1692508148000},"score":1,"resource":{"primary":{"URL":"https:\/\/currentprotocols.onlinelibrary.wiley.com\/doi\/10.1002\/cphg.38"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,4]]},"references-count":14,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2017,4]]}},"alternative-id":["10.1002\/cphg.38"],"URL":"https:\/\/doi.org\/10.1002\/cphg.38","archive":["Portico"],"relation":{},"ISSN":["1934-8266","1934-8258"],"issn-type":[{"value":"1934-8266","type":"print"},{"value":"1934-8258","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,4]]}}}