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Of the murine models used to study the pathophysiology of sepsis, CLP combines tissue necrosis and polymicrobial sepsis secondary to autologous fecal leakage, as well as hemodynamic and biochemical responses similar to those seen in septic humans. Further, a transient numerical reduction of multiple immune cell types, followed by development of prolonged immunoparalysis, occurs in CLP\u2010induced sepsis just as in humans. Use of the CLP model has led to a vast expansion in knowledge regarding the intricate physiological and cellular changes that occur during and after a septic event. This updated article details the steps necessary to perform this survival surgical technique, as well as some of the obstacles that may arise when evaluating the sepsis\u2010induced changes within the immune system. It also provides representative monoclonal antibody (mAb) panels for multiparameter flow cytometric analysis of the murine immune system in the septic host. \u00a9 2020 Wiley Periodicals LLC.<\/jats:p><jats:p><jats:bold>Basic Protocol<\/jats:bold>: Cecal ligation and puncture in the mouse<\/jats:p>","DOI":"10.1002\/cpim.110","type":"journal-article","created":{"date-parts":[[2020,10,7]],"date-time":"2020-10-07T22:33:24Z","timestamp":1602110004000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":46,"title":["Inducing Experimental Polymicrobial Sepsis by Cecal Ligation and Puncture"],"prefix":"10.1002","volume":"131","author":[{"given":"Frances V.","family":"Sjaastad","sequence":"first","affiliation":[{"name":"Microbiology, Immunology, and Cancer Biology Ph.D. Program University of Minnesota  Minneapolis Minnesota"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isaac J.","family":"Jensen","sequence":"additional","affiliation":[{"name":"Interdisciplinary Graduate Program in Immunology University of Iowa  Iowa City Iowa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roger R.","family":"Berton","sequence":"additional","affiliation":[{"name":"Interdisciplinary Graduate Program in Immunology University of Iowa  Iowa City Iowa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vladimir P.","family":"Badovinac","sequence":"additional","affiliation":[{"name":"Interdisciplinary Graduate Program in Immunology University of Iowa  Iowa City Iowa"},{"name":"Department of Pathology University of Iowa  Iowa City Iowa"},{"name":"Department of Microbiology and Immunology University of Iowa  Iowa City Iowa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas S.","family":"Griffith","sequence":"additional","affiliation":[{"name":"Microbiology, Immunology, and Cancer Biology Ph.D. Program University of Minnesota  Minneapolis Minnesota"},{"name":"Department of Urology University of Minnesota  Minneapolis Minnesota"},{"name":"Center for Immunology University of Minnesota  Minneapolis Minnesota"},{"name":"Masonic Cancer Center University of Minnesota  Minneapolis Minnesota"},{"name":"Minneapolis VA Health Care System  Minneapolis Minnesota"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,10,7]]},"reference":[{"key":"e_1_2_3_2_1","doi-asserted-by":"publisher","DOI":"10.2174\/187153006777442332"},{"key":"e_1_2_3_3_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra1208623"},{"key":"e_1_2_3_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00011-006-5150-y"},{"key":"e_1_2_3_5_1","first-page":"331","article-title":"Evaluation of factors affecting mortality rate after sepsis in a murine cecal ligation and puncture model","volume":"94","author":"Baker C. 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