{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,13]],"date-time":"2026-06-13T13:01:07Z","timestamp":1781355667542,"version":"3.54.1"},"reference-count":22,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2021,11,30]],"date-time":"2021-11-30T00:00:00Z","timestamp":1638230400000},"content-version":"am","delay-in-days":365,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#am"},{"start":{"date-parts":[[2020,11,30]],"date-time":"2020-11-30T00:00:00Z","timestamp":1606694400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000072","name":"National Institute of Dental and Craniofacial Research","doi-asserted-by":"publisher","award":["DE025030"],"award-info":[{"award-number":["DE025030"]}],"id":[{"id":"10.13039\/100000072","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000072","name":"National Institute of Dental and Craniofacial Research","doi-asserted-by":"publisher","award":["DE029707"],"award-info":[{"award-number":["DE029707"]}],"id":[{"id":"10.13039\/100000072","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100001298","name":"Presbyterian Health Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100001298","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100008907","name":"Oklahoma Medical Research Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100008907","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["currentprotocols.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["CP in Immunology"],"published-print":{"date-parts":[[2020,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Sj\u00f6gren's syndrome (SS) is a systemic autoimmune disease affecting multiple organ systems. Salivary and lacrimal gland involvement cause dry mouth and dry eye and are the most common clinical presentations of the disease. Patients with SS also have autoantibodies targeting multiple nuclear and cytoplasmic antigens. Innate immune activation plays a critical role in SS pathogenesis. This article describes the activation of specific innate immune pathways in mice to study SS salivary gland manifestations. Methodologies for evaluating salivary gland inflammation and salivary function are described. This article also describes protocols for in\u2010house assays to measure autoantibody titers in serum. \u00a9 2020 Wiley Periodicals LLC<\/jats:p><jats:p><jats:bold>Basic Protocol 1<\/jats:bold>: Acceleration of Sj\u00f6gren's syndrome by activating the toll\u2010like receptor 3 pathway<\/jats:p><jats:p><jats:bold>Basic Protocol 2<\/jats:bold>: Induction of Sj\u00f6gren's syndrome by activating the stimulator of interferon genes pathway<\/jats:p><jats:p><jats:bold>Alternate Protocol<\/jats:bold>: Acceleration of Sj\u00f6gren's syndrome by the administration of Freund's incomplete adjuvant<\/jats:p><jats:p><jats:bold>Support Protocol 1<\/jats:bold>: Evaluating salivary gland function<\/jats:p><jats:p><jats:bold>Support Protocol 2<\/jats:bold>: Evaluating salivary gland inflammation<\/jats:p><jats:p><jats:bold>Support Protocol 3<\/jats:bold>: Measuring autoantibody titers by indirect immunofluorescence<\/jats:p>","DOI":"10.1002\/cpim.114","type":"journal-article","created":{"date-parts":[[2020,11,30]],"date-time":"2020-11-30T14:20:24Z","timestamp":1606746024000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Protocols for Experimental Sj\u00f6gren's Syndrome"],"prefix":"10.1002","volume":"131","author":[{"given":"Harini","family":"Bagavant","sequence":"first","affiliation":[{"name":"Arthritis and Clinical Immunology Oklahoma Medical Research Foundation  Oklahoma City Oklahoma"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Umesh S.","family":"Deshmukh","sequence":"additional","affiliation":[{"name":"Arthritis and Clinical Immunology Oklahoma Medical Research Foundation  Oklahoma City Oklahoma"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,11,30]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-54410-9"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaut.2012.05.008"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/0471142727.mb29a03s75"},{"key":"e_1_2_11_5_1","first-page":"57203","article-title":"A method for the measurement of salivary gland function in mice","volume":"131","author":"Bagavant H.","year":"2018","journal-title":"Journal of Visualized Experiments"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.10258"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2015.00412"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.23368"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/0471142735.im0108s73"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1136\/annrheumdis-2016-210448"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.1992.263.4.E607"},{"key":"e_1_2_11_12_1","first-page":"671","article-title":"A laboratory model for Sj\u00f6gren's syndrome","volume":"52","author":"Kessler H. 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