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This unit provides detailed protocols for growing this bacterium, infecting mice by intragastric inoculation, measuring bacterial loads in feces and organs, and monitoring intestinal pathology induced by infection. Additional protocols describe steps needed to create frozen stocks, establish a growth curve, perform <jats:italic>ex vivo<\/jats:italic> organ cultures, isolate immune cells from the large intestine, and measure immune response by flow cytometry. \u00a9 2017 by John Wiley &amp; Sons, Inc.<\/jats:p>","DOI":"10.1002\/cpim.34","type":"journal-article","created":{"date-parts":[[2017,11,1]],"date-time":"2017-11-01T14:10:57Z","timestamp":1509545457000},"source":"Crossref","is-referenced-by-count":61,"title":["The Mouse Model of Infection with <i>Citrobacter rodentium<\/i>"],"prefix":"10.1002","volume":"119","author":[{"given":"Nicolas","family":"Bouladoux","sequence":"first","affiliation":[{"name":"Mucosal Immunology Section, Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases  NIH Bethesda Maryland"},{"name":"NIAID Microbiome Program, National Institute of Allergy and Infectious Diseases  NIH Bethesda 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