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Lindsay Whitton. We provide an explanation of the LCMV infection models, highlighting the importance of selecting an appropriate route and viral strain. We also describe how to quantify virus\u2010specific immune responses, followed by an explanation of useful transgenic systems. Specifically, our article will focus on the following protocols. \u00a9 2020 Wiley Periodicals LLC.<\/jats:p><jats:p><jats:bold>Basic Protocol 1<\/jats:bold>: LCMV infection routes in mice<\/jats:p><jats:p><jats:bold>Support Protocol 1<\/jats:bold>: Preparation of LCMV stocks<\/jats:p><jats:p><jats:bold><jats:italic>ASSAYS TO MEASURE LCMV TITERS<\/jats:italic><\/jats:bold><\/jats:p><jats:p><jats:bold>Support Protocol 2<\/jats:bold>: Plaque assay<\/jats:p><jats:p><jats:bold>Support Protocol 3<\/jats:bold>: Immunofluorescence focus assay (IFA) to measure LCMV titer<\/jats:p><jats:p><jats:bold><jats:italic>MEASUREMENT OF T CELL AND B CELL RESPONSES TO LCMV INFECTION<\/jats:italic><\/jats:bold><\/jats:p><jats:p><jats:bold>Basic Protocol 2<\/jats:bold>: Triple tetramer staining for detection of LCMV\u2010specific CD8 T cells<\/jats:p><jats:p><jats:bold>Basic Protocol 3<\/jats:bold>: Intracellular cytokine staining (ICS) for detection of LCMV\u2010specific T cells<\/jats:p><jats:p><jats:bold>Basic Protocol 4<\/jats:bold>: Enumeration of direct ex vivo LCMV\u2010specific antibody\u2010secreting cells (ASC)<\/jats:p><jats:p><jats:bold>Basic Protocol 5<\/jats:bold>: Limiting dilution assay (LDA) for detection of LCMV\u2010specific memory B cells<\/jats:p><jats:p><jats:bold>Basic Protocol 6<\/jats:bold>: ELISA for quantification of LCMV\u2010specific IgG antibody<\/jats:p><jats:p><jats:bold>Support Protocol 4<\/jats:bold>: Preparation of splenic lymphocytes<\/jats:p><jats:p><jats:bold>Support Protocol 5<\/jats:bold>: Making BHK21\u2010LCMV lysate<\/jats:p><jats:p><jats:bold>Basic Protocol 7<\/jats:bold>: Challenge models<\/jats:p><jats:p><jats:bold><jats:italic>TRANSGENIC MODELS<\/jats:italic><\/jats:bold><\/jats:p><jats:p><jats:bold>Basic Protocol 8<\/jats:bold>: Transfer of P14 cells to interrogate the role of IFN\u2010I on CD8 T cell responses<\/jats:p><jats:p><jats:bold>Basic Protocol 9<\/jats:bold>: Comparing the expansion of na\u00efve versus memory CD4 T cells following chronic viral challenge<\/jats:p>","DOI":"10.1002\/cpim.99","type":"journal-article","created":{"date-parts":[[2020,7,7]],"date-time":"2020-07-07T00:44:46Z","timestamp":1594082686000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":39,"title":["Interrogating Adaptive Immunity Using LCMV"],"prefix":"10.1002","volume":"130","author":[{"given":"Tanushree","family":"Dangi","sequence":"first","affiliation":[{"name":"Microbiology\u2010Immunology Northwestern University Feinberg School of Medicine  Chicago Illinois"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Young Rock","family":"Chung","sequence":"additional","affiliation":[{"name":"Microbiology\u2010Immunology Northwestern University Feinberg School of Medicine  Chicago 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