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As development of novel antibiotics stalls, preserving the effectiveness of existing agents has become a priority. One of the major driving forces behind antimicrobial resistance is the misuse and overuse of antibiotics, often a result of data on the susceptibility of pathogens not being obtained in a convenient and timely manner, a need that conventional antimicrobial susceptibility testing struggles to meet. Here, a hydrogel microfluidic platform is reported for antimicrobial susceptibility testing purposes, capable of handling real samples and yielding results within 2.5\u2005h of culture. By using a multiplayer design with channels crossing overhead of each other, multiple experiments, either one\u2010 or two\u2010dimensional, can be staged on the same device. Bacteria grown on the surface of the hydrogel can be easily visualized with standard Gram staining after being transferred onto a glass slide. Coupled with software\u2010based image analysis, the system can yield a variety of useful information on bacterial susceptibility and the effects of drugs, such as minimum inhibitory concentration and morphological changes in bacteria, either individually or in combination. Compared to conventional testing methods, this system requires less labor, reagents, and equipment to operate, and has significantly higher speed and efficiency.<\/jats:p>","DOI":"10.1002\/cplu.201600654","type":"journal-article","created":{"date-parts":[[2017,5,17]],"date-time":"2017-05-17T00:42:44Z","timestamp":1494981764000},"page":"792-801","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["A Multiplexed, Gradient\u2010Based, Full\u2010Hydrogel Microfluidic Platform for Rapid, High\u2010Throughput Antimicrobial Susceptibility Testing"],"prefix":"10.1002","volume":"82","author":[{"given":"Zhengzhi","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Chemistry Hong Kong Baptist University  Waterloo Rd, Kowloon Hong Kong P. 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