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Single asymmetric nanofluidic channels were fabricated in polyethylene terephthalate (PET) membranes. The chelator (DPA\u2212NH<jats:sub>2<\/jats:sub>) molecules are subsequently immobilized on the nanochannel surface, followed by the zinc ion complexation to afford DPA\u2212Zn<jats:sup>2+<\/jats:sup> chelates, which act as ligand moieties for the specific binding of phosphoproteins. The success of the chemical reaction and biomolecular recognition process that occur in a confined geometry can be monitored from the changes in electrical readout of the nanochannel. The nanofluidic sensor has the ability to sensitively and specifically detect lower concentrations (\u22651\u2005nM) of phosphoprotein (albumin and \u03b1\u2010casein) in the surrounding environment as evidenced from the significant decrease in ion current flowing through the nanochannels. However, dephosphoproteins such as lysozyme and dephospho\u2010\u03b1\u2010casein even at higher concentration (&gt;1\u2005\u03bcM) could not induce any significant change in the transmembrane ion flux. This observation indicated the sensitivity and specificity of the proposed nanofluidic sensor towards PPn proteins, and has potential for use in differentiating between phosphoproteins and dephosphoproteins.<\/jats:p>","DOI":"10.1002\/cplu.202000045","type":"journal-article","created":{"date-parts":[[2020,2,17]],"date-time":"2020-02-17T06:23:06Z","timestamp":1581920586000},"page":"587-594","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Phosphoprotein Detection with a Single Nanofluidic Diode Decorated with Zinc Chelates"],"prefix":"10.1002","volume":"85","author":[{"given":"Saima","family":"Nasir","sequence":"first","affiliation":[{"name":"Technische Universit\u00e4t Darmstadt Fachbereich Material- u. 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Geowissenschaften Fachgebiet Materialanalytik  Alarich-Weiss-Str. 2 64287 Darmstadt Germany"},{"name":"GSI Helmholtzzentrum f\u00fcr Schwerionenforschung  Planckstr. 1 64291 Darmstadt Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ishtiaq","family":"Ahmed","sequence":"additional","affiliation":[{"name":"Karlsruhe Institute of Technology (KIT) Institute for Biological Interfaces (IBG-1)  Hermann-von-Helmholtz-Platz 76344 Eggenstein-Leopoldshafen Germany"},{"name":"University of Cambridge Department of Chemical Engineering and Biotechnology  Philippa Fawcett Drive Cambridge C B3 0AS United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christof M.","family":"Niemeyer","sequence":"additional","affiliation":[{"name":"Karlsruhe Institute of Technology (KIT) Institute for Biological Interfaces (IBG-1)  Hermann-von-Helmholtz-Platz 76344 Eggenstein-Leopoldshafen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wolfgang","family":"Ensinger","sequence":"additional","affiliation":[{"name":"Technische Universit\u00e4t Darmstadt Fachbereich Material- u. 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