{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T07:59:27Z","timestamp":1790409567088,"version":"4.1.0"},"reference-count":67,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2025,8,9]],"date-time":"2025-08-09T00:00:00Z","timestamp":1754697600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,8,9]],"date-time":"2025-08-09T00:00:00Z","timestamp":1754697600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["chemistry-europe.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["ChemPlusChem"],"published-print":{"date-parts":[[2025,10,9]]},"abstract":"<jats:p>\n                    Stereospecific synthesis of Methyl\u2010(\n                    <jats:italic>Z<\/jats:italic>\n                    )\u20102\u2010(7\u2010methyl\u20103\u2010oxo\u20105\n                    <jats:italic>H<\/jats:italic>\n                    \u2010thiazolo[3,2\u2010\n                    <jats:italic>a<\/jats:italic>\n                    ]pyrimidin\u20102(3\n                    <jats:italic>H<\/jats:italic>\n                    )\u2010ylidene)acetates\n                    <jats:bold>3<\/jats:bold>\n                    (\n                    <jats:bold>a\u2013<\/jats:bold>\n                    <jats:bold>t<\/jats:bold>\n                    ) is designed and explored their application as fluorescent probes for Cd\n                    <jats:sup>2+<\/jats:sup>\n                    ions for the first time. These analogs are synthesized via one\u2010pot reaction of (R\/S)\u20102\u2010thioxo\u20103,4\u2010dihydropyrimidine\u20105\u2010carboxanilides (TDHPM\u20105\u2010carboxanilides) with dimethyl acetylenedicarboxylate in MeOH (methanol) as a reusable green solvent at room temperature (RT). Two new C(sp\n                    <jats:sup>2<\/jats:sup>\n                    )\uf8ffN, C(sp\n                    <jats:sup>2<\/jats:sup>\n                    )\uf8ffS sigma bonds, and one new C\u2010N \u03c0\u2010bond are formed during this transformation, achieving yields of up to 99%. This protocol demonstrates a low environmental factor (E\u2010factor) (0.09 to 0.35), high atom economy (94.83%), and carbon efficiency (142.68%), reflecting a significant advancement in green chemistry. Compound\n                    <jats:bold>3a<\/jats:bold>\n                    is synthesized in 99% yield on a gram scale. Compound\n                    <jats:bold>3p<\/jats:bold>\n                    proves to be an excellent fluorescent probe for Cd\n                    <jats:sup>2+<\/jats:sup>\n                    ions, showing a ninefold increase in fluorescence intensity. The limit of detection and limit of quantification for\n                    <jats:bold>3p<\/jats:bold>\n                    with Cd\n                    <jats:sup>2+<\/jats:sup>\n                    ions are 7.5 and 25\u2009\u03bcM, respectively, with an R\n                    <jats:sup>2<\/jats:sup>\n                    value of 0.9903. Density functional theory calculations revealed that Cd\n                    <jats:sup>2+<\/jats:sup>\n                    ions bind effectively in the cavity of\n                    <jats:bold>3p<\/jats:bold>\n                    through a ligand\u2010to\u2010metal charge transfer mechanism. Cell viability assays and docking studies show that compounds\n                    <jats:bold>3g<\/jats:bold>\n                    ,\n                    <jats:bold>3h<\/jats:bold>\n                    ,\n                    <jats:bold>3p<\/jats:bold>\n                    , and\n                    <jats:bold>3t<\/jats:bold>\n                    exhibited potent activity against the DU145 prostate cancer cell line, with binding affinities of \u22127.7, \u22126.8, \u22128.1, and \u22127.5\u2009kcal\u2009mol\n                    <jats:sup>\u2212<\/jats:sup>\n                    <jats:sup>1<\/jats:sup>\n                    .\n                  <\/jats:p>","DOI":"10.1002\/cplu.202500266","type":"journal-article","created":{"date-parts":[[2025,7,10]],"date-time":"2025-07-10T20:14:45Z","timestamp":1752178485000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Room\u2010Temperature Accessible Stereospecific Synthesis of Methyl\u2010(\n                    <i>Z<\/i>\n                    )\u20102\u2010(7\u2010methyl\u20103\u2010oxo\u20105\n                    <i>H<\/i>\n                    \u2010thiazolo[3,2\u2010\n                    <i>a<\/i>\n                    ]pyrimidin\u20102(3\n                    <i>H<\/i>\n                    )\u2010ylidene)acetates:Cd\n                    <sup>2+<\/sup>\n                    Fluorescence Probe and DU145 Cell Study"],"prefix":"10.1002","volume":"90","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6958-8568","authenticated-orcid":false,"given":"Mehul P.","family":"Parmar","sequence":"first","affiliation":[{"name":"Department of Chemistry Sardar Patel University  Vallabh Vidyanagar Gujarat 388120 India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6979-3657","authenticated-orcid":false,"given":"Disha P.","family":"Vala","sequence":"additional","affiliation":[{"name":"Department of Chemistry Sardar Patel University  Vallabh Vidyanagar Gujarat 388120 India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-7500-9106","authenticated-orcid":false,"given":"Krushna A.","family":"Baraiya","sequence":"additional","affiliation":[{"name":"Department of Chemistry Sardar Patel University  Vallabh Vidyanagar Gujarat 388120 India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-2456-7951","authenticated-orcid":false,"given":"Savan S.","family":"Bhalodiya","sequence":"additional","affiliation":[{"name":"Department of Chemistry Sardar Patel University  Vallabh Vidyanagar Gujarat 388120 India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-1786-3475","authenticated-orcid":false,"given":"Chirag D.","family":"Patel","sequence":"additional","affiliation":[{"name":"Department of Chemistry Sardar Patel University  Vallabh Vidyanagar Gujarat 388120 India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pranathi","family":"Konda","sequence":"additional","affiliation":[{"name":"Faculty of Clinical Research Sri Ramachandra Institute of Higher Education &amp; Research Prostate Cancer Biomarker Laboratory  Porur Chennai 600116 India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7420-8459","authenticated-orcid":false,"given":"Vinoth Kumar","family":"Lakshmanan","sequence":"additional","affiliation":[{"name":"Faculty of Clinical Research Sri Ramachandra Institute of Higher Education &amp; Research Prostate Cancer Biomarker Laboratory  Porur Chennai 600116 India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0750-903X","authenticated-orcid":false,"given":"Pinkesh G.","family":"Sutariya","sequence":"additional","affiliation":[{"name":"Department of Chemistry Sardar Patel University  Vallabh Vidyanagar Gujarat 388120 India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4740-7329","authenticated-orcid":false,"given":"Hitendra M.","family":"Patel","sequence":"additional","affiliation":[{"name":"Department of Chemistry Sardar Patel University  Vallabh Vidyanagar Gujarat 388120 India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,8,9]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1039\/b700923b"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1021\/acssuschemeng.9b05184"},{"key":"e_1_2_7_4_1","first-page":"179","volume-title":"Advances in Heterocy clic Chemistry","author":"Vala R. 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